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Su W.-C.,Lovelace Respiratory Research Institute | Tolchinsky A.D.,Research Center for Toxicology and Hygienic Regulation of Biopreparations | Sigaev V.I.,Research Center for Toxicology and Hygienic Regulation of Biopreparations | Cheng Y.S.,Lovelace Respiratory Research Institute
Journal of the Air and Waste Management Association | Year: 2012

In this study, the performance of two newly developed personal bioaerosol samplers was evaluated. The two test samplers are cyclone-based personal samplers that incorporate a recirculating liquid film. The performance evaluations focused on the physical efficiencies that a personal bioaerosol sampler could provide, including aspiration, collection, and capture efficiencies. The evaluation tests were carried out in a wind tunnel, and the test personal samplers were mounted on the chest of a full-size manikin placed in the test chamber of the wind tunnel. Monodisperse fluorescent aerosols ranging from 0.5 to 20 mm were used to challenge the samplers. Two wind speeds of 0.5 and 2.0 m/sec were employed as the test wind speeds in this study. The test results indicated that the aspiration efficiency of the two test samplers closely agreed with the ACGIH inhalable convention within the size range of the test aerosols. The aspiration efficiency was found to be independent of the sampling orientation. The collection efficiency acquired from these two samplers showed that the 50% cutoff diameters were both around 0.6 mm. However, the wall loss of these two test samplers increased as the aerosol size increased, and the wall loss of PAS-4 was considerably higher than that of PAS-5, especially in the aerosol size larger than 5 mm, which resulted in PAS-4 having a relatively lower capture efficiency than PAS-5. Overall, the PAS-5 is considered a better personal bioaerosol sampler than the PAS-4. Implications: Detection of personal exposure to airborne microorganisms (bioaerosols) is essential for developing adequate environmental and occupational air monitoring programs. Recently, two liquid-cyclone-based personal bioaerosol samplers were developed for this purpose. This study is to evaluate the performance of these two newly developed samplers in terms of their physical sampling efficiencies, such as the aspiration, collection, and capture efficiencies. The results acquired can offer insight into the efficacy of the newly designed samplers on achieving the goal of providing reliable and accurate personal bioaerosol exposure assessment. © 2012 A&WMA.


Su W.-C.,Lovelace Respiratory Research Institute | Tolchinsky A.D.,Research Center for Toxicology and Hygienic Regulation of Biopreparations | Chen B.T.,Centers for Disease Control and Prevention | Sigaev V.I.,Research Center for Toxicology and Hygienic Regulation of Biopreparations | Cheng Y.S.,Lovelace Respiratory Research Institute
Journal of Environmental Monitoring | Year: 2012

The need to determine occupational exposure to bioaerosols has notably increased in the past decade, especially for microbiology-related workplaces and laboratories. Recently, two new cyclone-based personal bioaerosol samplers were developed by the National Institute for Occupational Safety and Health (NIOSH) in the USA and the Research Center for Toxicology and Hygienic Regulation of Biopreparations (RCT & HRB) in Russia to monitor bioaerosol exposure in the workplace. Here, a series of wind tunnel experiments were carried out to evaluate the physical sampling performance of these two samplers in moving air conditions, which could provide information for personal biological monitoring in a moving air environment. The experiments were conducted in a small wind tunnel facility using three wind speeds (0.5, 1.0 and 2.0 m s-1) and three sampling orientations (0°, 90°, and 180°) with respect to the wind direction. Monodispersed particles ranging from 0.5 to 10 μm were employed as the test aerosols. The evaluation of the physical sampling performance was focused on the aspiration efficiency and capture efficiency of the two samplers. The test results showed that the orientation-averaged aspiration efficiencies of the two samplers closely agreed with the American Conference of Governmental Industrial Hygienists (ACGIH) inhalable convention within the particle sizes used in the evaluation tests, and the effect of the wind speed on the aspiration efficiency was found negligible. The capture efficiencies of these two samplers ranged from 70% to 80%. These data offer important information on the insight into the physical sampling characteristics of the two test samplers. This journal is © The Royal Society of Chemistry 2012.


Tolchinsky A.D.,Research Center for Toxicology and Hygienic Regulation of Biopreparations | Sigaev V.I.,Research Center for Toxicology and Hygienic Regulation of Biopreparations | Varfolomeev A.N.,Research Center for Toxicology and Hygienic Regulation of Biopreparations | Uspenskaya S.N.,Research Center for Toxicology and Hygienic Regulation of Biopreparations | And 2 more authors.
Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering | Year: 2011

In this study, the performance of two newly developed personal bioaerosol samplers for monitoring the level of environmental and occupational airborne microorganisms was evaluated. These new personal bioaerosol samplers were designed based on a swirling cyclone with recirculating liquid film. The performance evaluation included collection efficiency tests using inert aerosols, the bioaerosol survival test using viable airborne microorganism, and the evaluation of using non-aqueous collection liquid for long-period sampling. The test results showed that these two newly developed personal bioaerosol samplers are capable of doing high efficiency, aerosol sampling (the cutoff diameters are around 0.7 μ m for both samplers), and have proven to provide acceptable survival for the collected bioaerosols. By using an appropriate non-aqueous collection liquid, these two personal bioaerosol samplers should be able to permit continuous, long-period bioaerosol sampling with considerable viability for the captured bioaerosols. © Taylor & Francis Group, LLC.

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