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Emami M.,University of Tehran | Shamsipur M.,Razi University | Saber R.,Tehran University of Medical Sciences | Saber R.,Research Center for Science and Technology in Medicine | Irajirad R.,Research Center for Science and Technology in Medicine

A label free immunosensor was designed for ultra-detection of human epidermal growth factor receptor 2 (HER2) in real samples using a differential pulse voltammetry (DPV) method. In a separate process, antiHER2 antibodies were attached to iron oxide nanoparticles (Fe3O4 NPs) to form stable bioconjugates which were later laid over the gold electrode surface. In this way, by the advantage of their long terminals, the bioconjugates provided the most possible space for the immuno-reaction between biomolecules. Under optimal conditions, the immunosensor was responsive to HER2 concentrations over the ranges of 0.01-10 ng mL-1 and 10-100 ng mL-1 linearly and benefited from a satisfactory detection limit as low as 0.995 pg mL -1 and a favorable sensitivity as sharp as 5.921 μA mL ng -1. The reliability of the method in clinical analysis was proved by successful quantization of HER2 levels in serum samples obtained from patients. Furthermore, the precision and the stability of the method were evaluated and verified to be acceptable in immunoassay studies. © the Partner Organisations 2014. Source

Emami M.,University of Tehran | Shamsipur M.,Razi University | Saber R.,Tehran University of Medical Sciences | Saber R.,Research Center for Science and Technology in Medicine
Journal of Solid State Electrochemistry

The present paper describes a sensitive electrochemical detection of amlodipine (AMLO) at the poly-l-methionine-gold nanoparticles/multi-walled carbon nanotube modified glassy carbon electrode (PLM-GNPs/MWCNTs/GCE) by differential pulse voltammetry (DPV) technique at physiological pH 7.12. Cyclic voltammetry results demonstrate that the proposed electrode shows excellent electrocatalytic activity toward oxidation of AMLO. Kinetic parameters of the electrochemical reaction are calculated, and analytical variables such as MWCNT volumes, drug accumulation time, electropolymerization cycles and pH values are also optimized. Under optimal conditions, the linear range covering from 5 nM to 2.5 μM along with detection limit of 1 nM is obtained. Moreover, this method is successfully used to detect AMLO in pharmaceutical samples and biological fluids of a dosage received by the volunteer. © 2013 Springer-Verlag Berlin Heidelberg. Source

Ai J.,Research Center for Science and Technology in Medicine | Javidan A.N.,Tehran University of Medical Sciences | Mehrabani D.,Shiraz University of Medical Sciences
Iranian Red Crescent Medical Journal

In the last few decades, the idea of being able to repair the brain by introducing new cells to repair the damaged areas has become an accepted potential treatment for neurodegenerative diseases. The stromal cell fraction of many tissues and organs has shown in vitro neurogenic differentiation; however, these cell types are limited by availability, invasiveness of extraction and in some cases limited proliferative capacity. Human endometrial adult stem cells have many clinical advantages over the other stem cells. Here, we propose the hypothesis that endometrial adult stem cells may be induced into neural cells. © Iranian Red Crescent Medical Journal. Source

Oghabian M.A.,Tehran University of Medical Sciences | Oghabian M.A.,Research Center for Science and Technology in Medicine | Farahbakhsh N.M.,Research Center for Science and Technology in Medicine
Journal of Biomedical Nanotechnology

The development of molecular imaging technologies represents the opportunity to differentiate tissues based upon their metabolic and functional activity rather than structural and anatomic characteristics. The goal of molecular imaging is to reveal the pathophysiology underlying the observed anatomy and to diagnose disease based upon early biochemical processes. Detection of pathologic biomarkers can lead to early recognition of diseases, better therapeutic management, and improved monitoring for recurrence. Among the current clinical imaging modalities, MRI is uniquely suited for molecular imaging applications, offering a non-invasive means to obtain both anatomic and metabolic/functional information with high spatial and temporal resolution. Site-specific MRI contrast agents have been developed to target biologic processes that occur early in the development of diseases such as atherosclerotic plaques, tumor angiogenesis, and disease specific biosignatures. Furthermore, early disease recognition, prompting therapeutic intervention and drug delivery evaluation are possible using targeted contrast agents. Copyright © 2010 American Scientific Publishers All rights reserved. Source

Marsousi M.,Research Center for Science and Technology in Medicine
Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference

In this paper, a fully automated method for segmenting Left Ventricle (LV) in echocardiography images is proposed. A new method named active ellipse model is developed to automatically find the best ellipse inside the LV chamber without intervention of any specialist. A modified B-Spline Snake algorithm is used to segment the LV chamber in which the initial contour is formed by the predefined ellipse. As a result of using active ellipse model, the segmentation is extricated from dealing with gaps within myocardium boundary which are highly problematic in echocardiography image segmentation. Based on the results obtained from different studies, the proposed method is faster and more accurate than previous approaches. Our method is evaluated on 20 sets of echocardiography images of patients; and acquired results (92.30 ± 4.45% dice's coefficient) indicate the proposed method has remarkable performance. Source

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