Research Center for science and Biotechnology

Tehrān, Iran

Research Center for science and Biotechnology

Tehrān, Iran

Time filter

Source Type

Kashani H.H.,Kashan University of Medical Sciences | Nikzad H.,Kashan University of Medical Sciences | Mobaseri S.,University of Qom | Hoseini E.S.,Research Center for science and Biotechnology
Life Science Journal | Year: 2012

Due to increasing interest to natural preservatives, many studies have been performed in recent years. Nisin peptide as a natural preservative is very interesting for the control of food pathogens and microorganisms of food spoilage. In this study antibacterial activity of nisin and its synergism in decreasing concentrations of common chemical preservatives like sodium nitrite and benzoic acid against standard bacteria of Staphylococcus aureus and Lysteria monocytogenes has been investigated. For this purpose, the MIC (minimum inhibitory concentration) of nisin peptide, sodium nitrite and benzoic acid were evaluated at different pH based on the dilution method. Results showed that these compounds have antibacterial activity against Staphylococcus aureus and Lysteria monocytogenes. MIC for nisin peptide, sodium nitrite and benzoic acid in Staphylococcus aureus, was 350, 200, 25 (ppm) respectively. These values were 100, 200, 10 (ppm) in Lysteria monocytogenes. It has been also shown that simultaneous use of nisin with chemical preservatives sodium nitrite and benzoic acid reduced MIC of these compounds against two strains of bacteria. This synergistic effect of nisin could reduce the use of chemical preservatives in food industry.


Seyed Hosseini E.,Research Center for science and Biotechnology | Zeinoddini M.,Research Center for science and Biotechnology | Haddad Kashani H.,Kashan University of Medical Sciences | Ghoddusi A.,Research Center for science and Biotechnology
Life Science Journal | Year: 2012

Inteins are parts of proteins that cut themselves out of the whole protein entirely on their own. An important development in the area of recombinant protein purification has been the incorporation of self-cleaving protein elements into a variety of fusion-based purification systems. Inteins appear most frequently in Archaea, but they are found in organisms belonging to all three domains of life and in viral and phage proteins. Most inteins consist of two domains: One is involved in autocatalytic splicing, and the other is an endonuclease that is important in the spread of inteins. This review focuses on the evolution and technical application of inteins and only briefly summarizes recent advances in the study of the catalytic activities and structures of inteins. We further investigated the recent expanded applications of the Express Protein Ligation (EPL) technology in the fields of proteomics and bioimaging. This work is expected to provide a rough outline for the evaluation of these methods for large-scale bioprocessing of a variety of products.


Seyed Hosseyni E.,Research Center for science and Biotechnology | Haddad Kashani H.,Kashan University of Medical Sciences | Hossein Asadi M.,Baqiyatallah Medical Sciences University
Life Science Journal | Year: 2012

Diabetes mellitus is a systemic metabolic disease characterized by hyperglycemia, hyperlipedemia, hyperaminoacidemia, and hypoinsulinaemia it leads to decrease in both insulin secretion and insulin action, along with varying degrees of peripheral resistance to the action of insulin. The long-term effects of impaired glucose regulation can lead to permanent organ damage, such as cardiovascular disease, and disabilities. Nowadays, there is growing interest in medicinal herbs due to the side effects associated with the therapeutic agent for the treatment of diabetes mellitus. In addition therapeutic action of herbal medicines is due to the herbal ingredients mechanism, bioactive compounds of most of the plants have been isolated and identified. However, mechanisms of action of most plants and their products that used for lowering of blood glucose remain unknown. in this study by searching in different sources and references such as Pub Med, MEDLINE, CNKI, EMBASE, Wiley Inter Science, Elsevier data bases, tried to express mode of action some common medicinal herbs that have important role in lowering of blood glucose and diabetics recovery.


Kashani H.H.,Kashan University of Medical Sciences | Hoseini E.S.,Research Center for science and Biotechnology | Nikzad H.,Kashan University of Medical Sciences | Aarabi M.H.,Kashan University of Medical Sciences
Life Science Journal | Year: 2012

Use of herbs based drugs and chemicals for treating various diseases are as old as human civilization. Herbs have vast ability to synthesize aromatic materials mainly secondary metabolites. Herbs and herbs-based therapies are the source of various modern pharmaceuticals. In many cases, these herbal materials serve as defensive molecules against microorganisms, insects, and herbivores. Further, some of which may involve in plant aroma (terpenoids), pigmentation (tannins and quinines), and flavor (ginger). The aim of this review was to study of secondary metabolites and bioactive chemical constituents of medicinal herbs and their pharmacological activity. Regarding our purpose we searched at Pub Med, MEDLINE, CNKI, EMBASE, Wiley Inter Science, Elselvier databases without language limitation. In this sense we applied different words related to herbal therapy, pharmacology, secondary metabolites and phytochemistry.


PubMed | Research Center for science and Biotechnology
Type: Evaluation Studies | Journal: Current microbiology | Year: 2011

The XylR regulatory protein is a transcriptional activator from the TOL plasmid of Pseudomonas putida mt-2 that is involved in the toluene and benzene degradation pathway. Here we describe the construction and laboratory characterization of recombinant biosensors (pGLPX plasmids) based on XylR and its cognate promoter (Pu). In the pGLPX plasmid, the reporter luc gene is under the control of the Pu promoter. We evaluated the ability of two distinct nucleotide sequences to function as SD elements and improve sensitivity of bioreporting. We also evaluated the effect of introducing the Trrn terminator on the specificity of the construct. E. coli transformed with pGLPX plasmids were used to sense toluene and its derivatives. The pattern of induction was different for each derivative. In general, more luciferase activity was induced by toluene and benzene than by TNT and DNT at most tested concentrations. The bioluminescence response of the reporter strains to the nitrotoluenes was significantly stronger at lower concentrations ( 50 mol) than at higher concentrations. Our results show that the SD sequence (taaggagg) is crucially important for biosensor sensitivity. The presence of the Trrn terminator in the bioreporter plasmid prevents nonspecific responses and also reduces biosensor sensitivity upon exposure to inducers. These data suggest that pGLPX strains can be used as whole-cell biosensors to detect toluene and related compounds. Further investigation will be required to optimize the application of pGLPX biosensors.

Loading Research Center for science and Biotechnology collaborators
Loading Research Center for science and Biotechnology collaborators