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Abramov V.,Institute of Immunological Engineering | Khlebnikov V.,Institute of Immunological Engineering | Kosarev I.,Institute of Immunological Engineering | Bairamova G.,Research Center for Obstetrics | And 10 more authors.
Probiotics and Antimicrobial Proteins | Year: 2014

Lactobacillus crispatus 2029 isolated upon investigation of vaginal lactobacilli of healthy women of reproductive age was selected as a probiotic candidate. The aim of the present study was elucidation of the role of L. crispatus 2029 in resistance of the female reproductive tract to genitourinary pathogens using cervicovaginal epithelial model. Lactobacilluscrispatus 2029 has surface layers (S-layers), which completely surround cells as the outermost component of their envelope. S-layers are responsible for the adhesion of lactobacilli on the surface of cervicovaginal epithelial cells. Study of interactions between L. crispatus 2029 and a type IV collagen, a major molecular component of epithelial cell extracellular matrix, showed that 125I-labeled type IV collagen binds to lactobacilli with high affinity (Kd = (8.0 ± 0.7) × 10−10 M). Lactobacilluscrispatus 2029 consistently colonized epithelial cells. There were no toxicity, epithelial damage and apoptosis after 24 h of colonization. Electronic microscope images demonstrated intimate association between L. crispatus 2029 and epithelial cells. Upon binding to epithelial cells, lactobacilli were recognized by toll-like 2/6 receptors. Lactobacilluscrispatus induced NF-κB activation in epithelial cells and did not induce expression of innate immunity mediators IL-8, IL-1β, IL-1α and TNF-α. Lactobacilluscrispatus 2029 inhibited IL-8 production in epithelial cells induced by MALP-2 and increased production of anti-inflammatory cytokine IL-6, maintaining the homeostasis of female reproductive tract. Lactobacilluscrispatus 2029 produced H2O2 and provided wide spectrum of antagonistic activity increasing colonization resistance to urinary tract infections by bacterial vaginosis and vulvovaginal candidiasis associated agents. © 2014, Springer Science+Business Media New York.

Larina I.M.,Russian Academy of Sciences | Pastushkova L.K.,Russian Academy of Sciences | Tiys E.S.,RAS Institute of Cytology and Genetics | Kireev K.S.,Russian Academy of Sciences | And 10 more authors.
Journal of Bioinformatics and Computational Biology | Year: 2015

Urinary proteins serve as indicators of various conditions in human normal physiology and disease pathology. Using mass spectrometry proteome analysis, the permanent constituent of the urine was examined in the Mars-500 experiment (520 days isolation of healthy volunteers in a terrestrial complex with an autonomous life support system). Seven permanent proteins with predominant distribution in the liver and blood plasma as well as extracellular localization were identified. Analysis of the overrepresentation of the molecular functions and biological processes based on Gene Ontology revealed that the functional association among these proteins was low. The results showed that the identified proteins may be independent markers of the various conditions and processes in healthy humans and that they can be used as standards in determination of the concentration of other proteins in the urine. © 2015 Imperial College Press.

Zinovyeva M.V.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Monastyrskaya G.S.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Kopantzev E.P.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Vinogradova T.V.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | And 6 more authors.
Diseases of the Esophagus | Year: 2010

Here we directly compared gene expression profiles in human esophageal squamous cell carcinomas and in human fetal esophagus development. We used the suppression subtractive hybridization technique to subtract cDNAs prepared from tumor and normal human esophageal samples. cDNA sequencing and reverse transcription polymerase chain reaction (RT-PCR) analysis of RNAs from human tumor and the normal esophagus revealed 10 differentially transcribed genes: CSTA, CRNN, CEACAM1, MAL, EMP1, ECRG2, and SPRR downregulated, and PLAUR, SFRP4, and secreted protein that is acidic and rich in cysteine upregulated in tumor tissue as compared with surrounding normal tissue. In turn, genes up- and downregulated in tumor tissue were down- and upregulated, respectively, during development from the fetal to adult esophagus. Thus, we demonstrated that, as reported for other tumors, gene transcriptional activation and/or suppression events in esophageal tumor progression were opposite to those observed during development from the fetal to adult esophagus. This tumor 'embryonization' supports the idea that stem or progenitor cells are implicated in esophageal cancer emergence. © 2009 Copyright the Authors, Journal compilation © 2010, Wiley Periodicals, Inc. and the International Society for Diseases of the Esopha.

Trufanov V.D.,Peoples Friendship University of Russia | Kogan E.A.,Moscow State University | Yutskovskaya Y.A.,Bldg | Faizullina N.M.,Research Center for Obstetrics | Ivanov S.Y.,Nizhny Novgorod State Medical Academy
Sovremennye Tehnologii v Medicine | Year: 2016

The aim of investigation was to evaluate the efficiency of high frequency radio wave exposure (4.0 MHz) for the correction of age-related changes of facial skin by studying aspects of its regeneration, on the basis of morphological and immunohistochemical characteristics. Materials and Methods. The study consisted of two parts. The clinical part included an analysis of the results of high frequency radio wave facial rejuvenation (4.0 MHz) (5 procedures: on days 1, 30, 60, and months 6, 12). Immunohistochemical investigation was performed on repeated punch-biopsy specimens of the skin behind the ear, before the exposure, and at 30, 60, 180 and 360 days after a single exposure. Collagen of types 1 and 3, elastin, Ki-67, CD34, SMA, MMP2 and TIMP1 were identified. Results. It was found that the most evident morphological changes after high frequency radio wave exposure occurred in the deeper dermis layers and in the adjacent adipose tissue. Such remodeling of the extracellular matrix of the dermis causes an expansion of the deep dermis layers with the accumulation of collagen of types 1 and 3 with a greater proportional increase in favor of type 1. The key anti-ageing factor of radio wave exposure is considered to be the activation of neoangiogenesis in the dermis, which occurs gradually, reaching its maximum by month 12 after a single exposure. © 2016, Nizhny Novgorod State Medical Academy. All rights reserved.

Bourmenskaya O.,Research Center for Obstetrics | Shubina E.,Moscow Institute of Physics and Technology | Trofimov D.,Moscow Institute of Physics and Technology | Rebrikov D.,Moscow Institute of Physics and Technology | And 4 more authors.
Journal of Clinical Pathology | Year: 2013

Aims Uterine cervical carcinoma (CC) is known to be a delayed consequence of human papillomavirus (HPV) infection. Considering the reported influence of HPV on host genome activity, we conceived an approach to capture human gene expression profiles corresponding to increased risks of carcinogenesis. Methods A sample set of 143 female participants included a 'control' group of 23, a 'pathology' group of 83 (cervical abnormalities of varied grade including 10 cases of CC), and a 'HPV carrier' group of 37 (infected but manifesting normal cytology). HPV detection, viral load measurements and gene expression profiling were performed by real-time PCR assays. Results Gradual increase in expression of proliferation markers and a decrease in expression of proapoptotic genes, some receptors, PTEN and PTGS2 were demonstrated for progressive grades of cervical intraepithelial neoplasia leading to cancer. All reported trends were statistically significant, for instance, correlation of gene expression values for MKI67, CCNB1 and BIRC5. A model was proposed that employed mRNA concentrations for genes MKI67, CDKN2A, PGR and BAX. Prompt distinction between the norm and the cancer, provided by initial calculation, suggested that positive values of the function could indicate the higher individual risks. Indeed, all patients assigned to high risk by calculation were HPV infected and showed elevated viral E6, E7 mRNA concentration known to be associated with CC onset. Conclusions The research was concentrated on dynamical gene expression profiling upon pathological changes ultimately leading to CC. Differences of normalised mRNA concentrations were used for quantitative model design and its primary approbation.

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