Research Center for Microscopic Morphology and Immunology

Romania

Research Center for Microscopic Morphology and Immunology

Romania

Time filter

Source Type

Gheorghisor I.,University of Medicine and Pharmacy of Craiova | Balta F.L.,Carol Davila University of Medicine and Pharmacy | Mogoanta L.,Research Center for Microscopic Morphology and Immunology | Bold A.,University of Medicine and Pharmacy of Craiova
Romanian Journal of Morphology and Embryology | Year: 2013

Ultraviolet (UV) radiation in high doses may have harmful effects on the eye. The sources of UV radiation are the sun, as well as some artificial sources such as UV lamps or voltaic arcs. Chronic exposure to UV can cause damage to the anterior pole of the eye, ranging from minor (pterygium) to serious photokeratitis. In our study, we applied a UV dose of 6.5 J/cm2 in the wavelength range of 290-400 nm, for five consecutive days per rat anterior pole of the eye. Seven days after the last dose of radiation, the animals were sacrificed, harvesting both the irradiated and the non-irradiated eye. Histological and immunohistochemical examination of the lesions revealed that the greatest damage to the epithelium was recorded prior to and 2/3 of the remaining corneal stroma. The epithelial lesions we found varied from pseudokeratosis and detachment of the Bowman epithelium membrane to deep epithelial necrosis. Within the corneal stroma, we observed the formation of interstitial edema with disruption of the collagen structure. We also noticed the presence of an inflammatory infiltrate composed mainly of lymphocytes and CD68± and CD163± macrophages, as well as the occurrence of vascular devices. These consisted of angiogenesis capillaries with structured wall composed mainly of endothelial CD34± precursor cells and a basal membrane rich in collagen IV fibers.


Popescu F.C.,Research Center for Microscopic Morphology and Immunology | Busuioc C.J.,Research Center for Microscopic Morphology and Immunology | Pop O.T.,Research Center for Microscopic Morphology and Immunology | Parvanescu H.,University of Medicine and Pharmacy of Craiova | And 2 more authors.
Studia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii | Year: 2011

In the European ethnopharmacology, elder flowers are used external in poultices for the treatment of burns and blisters. Sambuci flos tincture was prepared and physico-chemically analyzed according to Romanian Pharmacopoeia. The soft extract, obtained from tincture, was embedded in an ointment base of cold cream type, prepared according to United States Pharmacopoeia. The study was performed on three groups of adult male Wistar rats, each of ten animals, kept under standard conditions, both before and after the burns. Skin burns from the dorsal region were treated daily as follows: cold cream with 10% Sambuci flos extract (SFE) for the first group; 1% silver sulfadiazine cream (SDA) for the second group; the third group, considered as control, received the cold cream base (CCB). The favorable evolution of burnt skin wounds in the SFE group, compared to SDA and CCB groups, is mainly due to the astringent, anti-inflammatory, antiseptic and cicatrizing action of the natural product. ©2011 Vasile Goldis University Press.


Stoiculescu A.,Emergency County Hospital | Pop O.T.,Research Center for Microscopic Morphology and Immunology | Alexandru D.O.,University of Medicine and Pharmacy of Craiova | Man M.,Emergency County Hospital
Romanian Journal of Morphology and Embryology | Year: 2012

The authors made a preliminary assessment of possible correlations between the amount of intratumoral stromal fibrillary components (ISFC) and the architectural tumoral patterns described by Gleason. The studied material consisted of samples obtained by transurethral resection from 34 patients diagnosed with prostatic adenocarcinoma. Ten fields, five for dominant and five for secondary identified patterns of each case, with no necrosis were selected randomly from Gömöri stained sections using ×20 objective. ISFC-ratio increased with Gleason pattern both for the entire group but also for "Necrotizing" phenotype patterns and "Solid" phenotype patterns, excepting the subtype "4A" where the stromal compartment was reduced by the expansion of tumoral ducts enlarged by growing tumoral intraductal cribriform masses. These preliminary data showed that stromal microenvironment try to adapt to the loss of tumoral differentiation by increasing the amount of fibrillary components of intratumoral stromal compartment.


Pop O.T.,Research Center for Microscopic Morphology and Immunology | Cotoi C.G.,University of Medicine and Pharmacy of Craiova | Plesea I.E.,University of Medicine and Pharmacy of Craiova | Gherghiceanu M.,Victor Babes National Institute | And 4 more authors.
Romanian Journal of Morphology and Embryology | Year: 2011

The authors evaluated possible morphological changes of basement membrane (BM) and lamina propria (LP) of seminferous tubule wall (ST) related to ageing. Surgical samples of testicular tissue from 28 cases with orchiectomy for prostate adenocarcinoma were processed for light microscopy and transmission electron microscopy (TEM) examination. Seven age groups (AgGr) between 50 and 80 years were designed. Tissue samples were immunomarked for collagen IV and smooth muscle actin. Images were acquired and measured with a specialized software. Thirty ST were randomly selected, with ×40-objective, for each case. Five random determinations for each ST and each parameter were performed. Mean values/tubule, case and AgGr were calculated for each parameter. Regression line (RL), slope and significance test for slope were determined for each parameter correlation with ageing. BM mean value was around 0.5 μm, with narrow limits of ranging in AgGr but more extended individual limits. RL showed discrete decreasing trend with ageing but without an obvious statistical correlation. LP mean value was around 6 μm, also with narrow limits of ranging in AgGr and more extended individual limits. RL decreased discretely with ageing but without an obvious statistical correlation. TEM showed more prominent BM material and more collagen fibers and less fibroblasts in LP of older AgGr and higher fibroblasts density in LP of younger AgGr. Our results showed that BM thickness is apparently decreasing with ageing whereas LP presents extremely variable degenerative changes, with a "mosaic", focal distribution and no tendency to advance with ageing.


Busuioc C.J.,Research Center for Microscopic Morphology and Immunology | Popescu F.C.,Research Center for Microscopic Morphology and Immunology | Mogosanu G.D.,University of Medicine and Pharmacy of Craiova | Lascar I.,Carol Davila University of Medicine and Pharmacy | And 3 more authors.
Romanian Journal of Morphology and Embryology | Year: 2011

In the past 30 years, after the discovery of vascular proliferation factors, angiogenesis is one of the most intensively studied fields. Restoring the vascular network after burn injury is essential for healing, as it brings oxygen and nutrients to injured tissues, improves the contribution of inflammatory cells and prepares the damaged area for repair and tissue regeneration. To study the process of angiogenesis we used seven groups of five animals, each of adult Wistar rats, which were inflicted with third degree skin burns. From each group of animals, we sampled at successive intervals of three days the entire burnt wound with a ring of surrounding normal skin. Sampled skin fragments were processed for paraffin inclusion, sectioned with a microtome and tained with Hematoxylin-Eosin or Masson trichrome. The samples were also analyzed using single chromogenic immunohistochemistry or double immunofluorescence for the presence of CD34 and alpha smooth muscle actin (α-SMA). Angiogenesis process started at about three days after the burn infliction, with the appearance of tubular structures lined by CD34-positive cells. Subsequently, these cells showed intense proliferative activity that generated a network that included progressive neovascularization around the wound surface. Maximum vascular proliferation occurred at 9-15 days after injury, when the number of capillaries reached 229/mm 2, and the total area of capillary angiogenesis at 100.27 μm 2 (about 10% of the section area). Subsequently, the process of angiogenesis was gradually reduced, but remained at moderate levels after wound healing. During the process of angiogenesis, there was a very close relationship between CD34-positive cells and pericytes (as α-SMApositive).


Stanciu G.,County Hospital | Mogoanta C.A.,University of Medicine and Pharmacy of Craiova | Ionita E.,University of Medicine and Pharmacy of Craiova | Timnea O.C.,Ecological University of Bucharest | And 4 more authors.
Romanian Journal of Morphology and Embryology | Year: 2011

Chronic rhinosinusitis is a multifactorial disease with pathophysiological mechanisms, which remain unclear, and with a high prevalence worldwide. They generate social problems due to the high number of days of leave and relatively elevated medical expenses. The histopathological and immunohistochemical study that we conducted revealed many lesional aspects of the epithelium of the sinus mucosa, which ranged from hypertrophy, hyperplasia and metaplasia, to erosion and discontinuities. In the chorion of the sinus mucosa there was an inflammatory infiltrate composed mainly of lymphocytes, plasma cells and macrophages, and also a highly developed vascular network. Among immune cells, T-cells appeared to be more numerous than B-lymphocytes and macrophages. We believe that microscopic changes are due mainly to microscopic organisms that make up the biofilm of the sinus cavity, whose virulence has been more or less influenced by exogenous or endogenous factors.

Loading Research Center for Microscopic Morphology and Immunology collaborators
Loading Research Center for Microscopic Morphology and Immunology collaborators