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Zhao-Ming Q.,Northeast Agricultural University | Ya-Nan S.,Northeast Agricultural University | Qiong W.,Northeast Agricultural University | Chun-Yan L.,Northeast Agricultural University | And 3 more authors.
Canadian Journal of Plant Science | Year: 2011

An integrated map of QTLs related to seed protein concentration in soybean has been constructed, based on the public genetic map, soymap2 as a reference map, along with a set of 107 QTLs reported in the literature over the past 20 yr. Each of these QTLs was projected onto the soymap2 by software package BioMercator v2.1. Twenty-three consensus QTLs were detected. The confidence interval at all sites ranged from 1.52 to 14.31cM, and the proportion of the phenotypic variance associated with each of them from 1.5 to 20.8%. Major chromosomal sites were identified on LG I (Gm20), four important sites were identified, involving LG A1 (Gm05), B2 (Gm14), E (Gm07) and M (Gm15). A meta-analysis approach was used to improve the precision of the location of these sites. These results facilitate gene mining and molecular assist-selection in soybean.

Qi Z.,Northeast Agricultural University | Wu Q.,Northeast Agricultural University | Han X.,Research Center for Crop Breeding of Hei Long jiang Land Reclamation | Sun Y.,Northeast Agricultural University | And 6 more authors.
Euphytica | Year: 2011

Oil content of soybean was a valuable quantitative trait controlled by multiple genes. Eleven QTLs were detected by both CIM and MIM method with the population crossed between Charleston and Dong nong594 in recent 3 years (2007, 2008, 2009). Combining the QTLs collected over the past 20 years, an integrated map of oil-content major QTLs in soybean was established using soymap2, which was published in 2004, as a reference. Using the software BioMercator ver. 2.1, QTLs were projected from their own maps onto the reference map. In total, ninety-eight QTLs were integrated into soymap2. A meta-analysis method was used to narrow down the confidence interval, and 20 consensus QTLs and their corresponding markers were obtained. Using a local version of GENSCAN, 10,137 sequences in the consensus QTL intervals were predicted. With BLAST, these predicted genes were compared to the International Protein Index database to mine the related genes. The results offer a basis for gene mining and molecular breeding in soybean. © 2011 Springer Science+Business Media B.V.

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