Research Center for Bioorganic Chemistry

Bangkok, Thailand

Research Center for Bioorganic Chemistry

Bangkok, Thailand

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Buchatip S.,Chulalongkorn University | Ananthanawat C.,Chulalongkorn University | Sithigorngul P.,Srinakharinwirot University | Sangvanich P.,Research Center for Bioorganic Chemistry | And 2 more authors.
Sensors and Actuators, B: Chemical | Year: 2010

An immunosensor based on a quartz crystal microbalance (QCM) was developed for detection of Vibrio harveyi (V. harveyi), a pathogenic bacteria causing morbidity and even a high level of fatality in commercial shrimp cultures, including Penaeus monodon. Immobilization of a monoclonal antibody (MAb) against V. harveyi onto the gold electrode of a QCM plate involved a three-step procedure: (1) the formation of a self-assembled monolayer (SAM) of a carboxyl-terminated alkanethiol, (2) activation of the carboxyl groups by N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and (3) attachment of the MAb to the activated carboxyl groups. The developed QCM-based immunosensor could be used for the detection of V. harveyi in a working range of 103-107 CFU/mL, with no detectable cross-reactivity to Vibrio vulnificus and Vibrio parahaemolyticus. Controlling the density of surface-immobilized MAb, by the formation of mixed SAMs containing hydroxyl-terminated alkanethiol in combination with the treatment of 1% (w/v) bovine serum albumin (BSA) as a blocking reagent, can significantly improve the binding efficiency of the targeted bacteria. © 2009 Elsevier B.V. All rights reserved.


Paebumrung P.,Chulalongkorn University | Petsom A.,Research Center for Bioorganic Chemistry | Petsom A.,Chulalongkorn University | Thamyongkit P.,Research Center for Bioorganic Chemistry | Thamyongkit P.,Chulalongkorn University
JAOCS, Journal of the American Oil Chemists' Society | Year: 2012

Novel bis(azo) dyes were successfully prepared from the coupling of either natural cardanol or its hydrogenated derivative with a series of diazotized aromatic amines and diamines. These new dyes were fully characterized by NMR, infrared and UV-visible spectroscopy and mass spectrometry. The dyes are highly soluble in a variety of common organic solvents and gasoline as a consequence of the cardanol unit. The physical properties of gasoline according to American Society for Testing and Materials standard were unaffected by the presence of the bis(azo) dye derived from 1-(4aminophenylazo)-2-naphthol at a concentration of 6 ppm and the dye showed excellent stability over a 3 month period. © AOCS 2011.


Naganagowda G.,Research Center for Bioorganic Chemistry | Thamyongkit P.,Research Center for Bioorganic Chemistry | Petsom A.,Research Center for Bioorganic Chemistry
Journal of the Chilean Chemical Society | Year: 2012

3-Chlorobenzothiophene-2-carbonyl chloride 1 was reacted with 4-aminoacetophenone in acetone to give compound 2. In order to prepare chalcones 3a-d, compound 2 was condensed with various aldehydes in the presence of KOH in DMF. These chalcones 3a-d on cyclization with urea, thiourea, hydroxylamine hydrochloride, phenyl hydrazines and hydrazine hydrate gave the corresponding oxapyrimidines 4a-d, isoxazolines 5a-d, pyrazoles 6a-h, pyrazolines 7a-d and thiopyrimidines 8a-d, respectively. The structures of all the synthesized compounds were confirmed by spectral data and had been screened for antibacterial activity.


Naganagowda G.,Research Center for Bioorganic Chemistry | Dom R.K.-U.,Research Center for Bioorganic Chemistry | Ariyakriangkrai W.,Research Center for Bioorganic Chemistry | Luechai A.,Research Center for Bioorganic Chemistry | Petsom A.,Research Center for Bioorganic Chemistry
Indian Journal of Heterocyclic Chemistry | Year: 2011

Chalcones, (2E)-3-(5-chloro-3-methyl-1-phenyl-1 H-pyrazol-4-yl)-1- phenylprop-2-en-1-ones 1a-b have been prepared from ketone and 5-chloro-3-methyl-1-phenyl-1H-pyrazole-4-carbaldehyde. These chalcones 1a-b on cyclization with urea, thiourea, hydroxylamine hydrochloride and phenyl hydrazine gave the corresponding oxopyrimidines 2a-b, thiopyrijnidines 5a-b, isoxazolines 3a-b and pyrazolines 4a-b respectively. The structures of all the synthesized compounds were confirmed by spectral data and have been screened for antibacterial, antifungal and analgesic activity.


Naganagowda G.,Research Center for Bioorganic Chemistry | Petsom A.,Research Center for Bioorganic Chemistry
Indian Journal of Heterocyclic Chemistry | Year: 2011

The condensation of 3-chloro-4-fluoroaniline 1 with chloroacetyl chloride gave 2-chloro-N-(3-chloro-4-fluorophenyl) acetamide 2. Compound 2 on treatment with KSCN yielded 3-(3-chloro-4-fluorophenyl)-2-imino-1,3-thiazolidin-4-one 3. Compound 3 on condensation with various aromatic aldehydes afforded a series of 5-arylidene-3-(3-chloro-4-fluorophenyl)-2-imino-1,3-thiazolin-4-ones 4a-i. The structures of all the synthesized compounds were confirmed by elemental analysis. IR, 1H NMR and mass spectral data and have been screened for antibacterial and antifungal activity.


Naganagowda G.,Research Center for Bioorganic Chemistry | Petsom A.,Research Center for Bioorganic Chemistry
Indian Journal of Heterocyclic Chemistry | Year: 2011

3-Chlorobenzothiophene-2-carbonyl chloride 1 was treated with l-(aminomethyl) cyclohexylacetic acid in acetone to get compound 2, compound 1 was also reacted with substituted 2-aminobenzothiazoles, carbohydrizeds, anilines and pyrazole to get 3a-d, 4a-d, 5a-f and 6. Compound 3-chlorobenzothiophene-2-carbohydrazide 7 reacted with pyrazole aldehyde and thiophene aldehyde to give 8a-c and 9 respectively. Compound 9 with acetic anhydride and PPE gave 10 and 11 respectively. The structures of newly synthesized compounds were elucidated on the basis of IR, 1H NMR and mass spectral data and have been screened for antimicrobial and analgesic activity.

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