Research Center En Alimentacion sarrollo Ac Ciad

Mazatlán, Mexico

Research Center En Alimentacion sarrollo Ac Ciad

Mazatlán, Mexico
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Puello-Cruz A.C.,Research Center En Alimentacion sarrollo Ac Ciad | Mezo-Villalobos S.,Research Center En Alimentacion sarrollo Ac Ciad
Journal of the World Aquaculture Society | Year: 2013

The objective of this research was to culture the calanoid copepod Pseudodiaptomus euryhalinus, Johnson 1939, and the harpacticoid Tisbe monozota, Bowman, 1962, in monospecific and combined cultures (P. euryhalinus:T. monozota at 1:1, 2:1, and 1:2 starting ratios), and to compare the nauplii, copepodite, and adult production. Mean total production ranged from 740.3±137.5 organisms/L (P. euryhalinus) to 884.3±489.7 organisms/L (T. monozota) for monospecific cultures. The 1:1 ratio mixed cultures gave 780.4±155.8 organisms/L, those with the 2:1 and 1:2 P. euryhalinus and T. monozota starting ratios produced 710.1±195.2 and 799.7±232.5 organisms/L, respectively, and there were no significant difference among treatments. All mixed cultures gave significantly lower copepodite productions than the monocultures of each species. In addition, the tendency to a decreased progeny production of the initial females of T. monozota indicates that the outcome of long-term mixed cultures would production either a high dominance of P. euryhalinus, or monocultures of this species. © Copyright by the World Aquaculture Society 2013.

Burgara-Estrella A.,Research Center En Alimentacion sarrollo Ac Ciad | Burgara-Estrella A.,Autonomous University of Barcelona | Diaz I.,Autonomous University of Barcelona | Rodriguez-Gomez I.M.,Autonomous University of Barcelona | And 4 more authors.
Viruses | Year: 2013

This work describes peptides from non-structural proteins (nsp) of porcine reproductive and respiratory syndrome virus (PRRSV) predicted as potential T cell epitopes by bioinfornatics and tested for their ability to induce IFN-γ and IL-10 responses. Pigs immunized with either genotype 1 or genotype 2 PRRSV attenuated vaccines (n=5/group) and unvaccinated pigs (n = 4) were used to test the peptides. Swine leukocyte antigen haplotype of each pig was also determined. Pigs were initially screened for IFN-γ responses (ELISPOT) and three peptides were identified; two of them in non-conserved segments of nsp2 and nsp5 and the other in a conserved region of nsp5 peptide. Then, peptides were screened for IL-10 inducing properties. Six peptides were found to induce IL-10 release in PBMC and some of them were also able to inhibit IFN-γ responses on PHA-stimulated cells. Interestingly, the IFN-γ low responder pigs against PRRSV were mostly homozygous for their SLA haplotypes. In conclusion, these results indicate that nsp of PRRSV contain T-cell epitopes inducing IFN-γ responses as well as IL-10 inducing segments with inhibitory capabilities. © 2013 by the authors; licensee MDPI, Basel, Switzerland.

Caceres-Martinez J.,CICESE | Caceres-Martinez J.,Institute Sanidad Acuicola | Ortega M.G.,Institute Sanidad Acuicola | Vasquez-Yeomans R.,Institute Sanidad Acuicola | And 3 more authors.
Journal of Invertebrate Pathology | Year: 2012

The mangrove oyster . Saccostrea palmula coexists with the pleasure oyster . Crassostrea corteziensis in coastal lagoons of northwest Mexico. Recent discovery of . Perkinsus marinus infecting the pleasure oyster in the region prompted evaluation of . S. palmula as an alternative . P. marinus host. An analysis to determine the possible presence of . P. marinus in natural and cultured populations of . S. palmula at four coastal lagoons in Sinaloa, Mexico was carried out during October-November 2010. Tissues from apparently healthy . S. palmula were evaluated using Ray's fluid thioglycollate method (RFTM), which revealed a . Perkinsus sp. to be present in all four locations at 6.7-20.0% prevalence. Histopathological analysis of these specimens showed tissue alterations and parasite forms consistent with moderate . P. marinus infection, which was confirmed by ribosomal non-transcribed spacer (NTS)-based PCR assays on DNA samples from oysters positive by RFTM and histology. DNA sequencing of amplified NTS fragments (307. bp) produced a sequence 98-100% similar to GenBank-deposited sequences of the NTS from . P. marinus. Fluorescent . in situ hybridization for . Perkinsus spp. and . P. marinus corroborated the PCR results, showing clear hybridization of . P. marinus in host tissues. This is the first record of . P. marinus infecting a species from genus . Saccostrea and the first record of the parasite from coastal lagoons in Sinaloa, Mexico. © 2012 Elsevier Inc..

Hernandez-Guzman R.,Research Center En Alimentacion sarrollo Ac Ciad | Ruiz-Luna A.,Laboratorio Of Manejo Ambiental
Journal of Hydroinformatics | Year: 2013

This paper introduces the Spatial Analysis of surface Runoff in ArcGis (SARA v1.0) interface to estimate curve number and runoff volume for hydrologic evaluations. This version runs in a vector platform as with other extensions of ArcGIS, introducing changes to the original Natural Resources Conservation Services-Curve Number method, and allowing adaptation to local conditions. The programming syntax was developed in Visual Basic 6.0 as an ActiveX Dynamic Link Library (DLL) to interact with ArcMap, and it is released as Free and Open Source Software (FOSS) that can be modified or upgraded to other programming languages. © IWA Publishing 2013.

Campos-Acevedo A.A.,National Autonomous University of Mexico | Garcia-Orozco K.D.,Research Center En Alimentacion sarrollo Ac Ciad | Sotelo-Mundo R.R.,Research Center En Alimentacion sarrollo Ac Ciad | Sotelo-Mundo R.R.,University of Sonora | Rudino-Pinera E.,National Autonomous University of Mexico
Acta Crystallographica Section F: Structural Biology and Crystallization Communications | Year: 2013

Thioredoxin (Trx) is a 12 kDa cellular redox protein that belongs to a family of small redox proteins which undergo reversible oxidation to produce a cystine disulfide bond through the transfer of reducing equivalents from the catalytic site cysteine residues (Cys32 and Cys35) to a disulfide substrate. In this study, crystals of thioredoxin 1 from the Pacific whiteleg shrimp Litopenaeus vannamei (LvTrx) were successfully obtained. One data set was collected from each of four crystals at 100 K and the three-dimensional structures of the catalytic cysteines in different redox states were determined: reduced and oxidized forms at 2.00 Å resolution using data collected at a synchrotron-radiation source and two partially reduced structures at 1.54 and 1.88 Å resolution using data collected using an in-house source. All of the crystals belonged to space group P3212, with unit-cell parameters a = 57.5 (4), b = 57.5 (4), c = 118.1 (8) Å. The asymmetric unit contains two subunits of LvTrx, with a Matthews coefficient (V M) of 2.31 Å3 Da-1 and a solvent content of 46%. Initial phases were determined by molecular replacement using the crystallographic model of Trx from Drosophila melanogaster as a template. In the present work, LvTrx was overexpressed in Escherichia coli, purified and crystallized. Structural analysis of the different redox states at the Trx active site highlights its reactivity and corroborates the existence of a dimer in the crystal. In the crystallographic structures the dimer is stabilized by several interactions, including a disulfide bridge between Cys73 of each LvTrx monomer, a hydrogen bond between the side chain of Asp60 of each monomer and several hydrophobic interactions, with a noncrystallographic twofold axis. © 2013.

Virgen-Ortiz J.J.,University of Colima | Ibarra-Junquera V.,University of Colima | Osuna-Castro J.A.,University of Colima | Escalante-Minakata P.,University of Colima | And 2 more authors.
Analytical Biochemistry | Year: 2012

One of the recurrent methodological problems in preparative biochemical work is the concentration of dilute protein solutions, including culture supernatants resulting from biotechnological processes. A procedure was developed to concentrate enzymes by a novel cryoconcentration system. This approach includes a new device that facilitates the sample freezing and the subsequent solute elution from the frozen matrix by centrifugation. The optimal centrifugation conditions for this cryoconcentration system were obtained using whey protein solution as a model. The procedure was applied to concentrate dilute solutions of commercial pectinase, measuring the endopolygalacturonase (EPG) activity of this enzyme in the concentrate by a method based on the on-line torque measurement, and of recombinant fructan:fructan 1-fructosyltransferase (1-FFT) protein of Pichia pastoris from a culture in a bioreactor, as an expression system. The optimal centrifugation speed, time, and temperature were 6150 g, 20 min, and 4 °C, respectively. The concentration factors for the dilute protein solutions were 9.2-, 11.2-, and 17.1-fold for 1-FFT, whey, and commercial pectinase, respectively. Recoveries ranged from 87% to 93%. The procedure allowed concentrating proteins efficiently without affecting their enzymatic activity. © 2012 Elsevier Inc. All rights reserved.

Rodriguez-Figueroa J.C.,Research Center En Alimentacion sarrollo Ac Ciad | Gonzalez-Cordova A.F.,Research Center En Alimentacion sarrollo Ac Ciad | Astiazaran-Garcia H.,Research Center En Alimentacion sarrollo Ac Ciad | Vallejo-Cordoba B.,Research Center En Alimentacion sarrollo Ac Ciad
British Journal of Nutrition | Year: 2013

Previous studies have demonstrated that milk fermented by specific Lactococcus lactis strains significantly inhibits the activity of angiotensin I-converting enzyme (ACE). However, the relationship between the ACE inhibitor and its in vivo action has revealed discrepancies. Therefore, the aim of the present study was to investigate the antihypertensive and heart rate (HR)-lowering effect of milk fermented by specific L. lactis in a murine model. Spontaneously hypertensive male rats (271 (SD14) g) were randomised into four treatment groups that were orally administered with milk fermented by L. lactis NRRL B-50 571 or L. lactis NRRL B-50 572 at 35 or 50 mg protein/kg body weight (BW), respectively. Further, two more groups were fed with different solutions as controls: a saline solution as the negative control and Captopril™ (40 mg/kg BW), a proven ACE inhibitor, as the positive control. Blood pressure and HR were monitored by the tail-cuff method before the treatments and at 2, 4, 6 and 24 h post-oral administration. The results demonstrated that milk fermented by L. lactis NRRL B-50 571 as well as by L. lactis NRRL B-50 572 presented an important systolic and diastolic blood pressure- and HR-lowering effect. Thus, milk fermented by specific L. lactis strains may present potential benefits in the prevention and treatment of CVD associated with hypertension in humans. Copyright © The Authors 2012.

Rascon-Castelo E.,Research Center En Alimentacion sarrollo Ac Ciad | Burgara-Estrella A.,Research Center En Alimentacion sarrollo Ac Ciad | Mateu E.,Autonomous University of Barcelona | Hernandez J.,Research Center En Alimentacion sarrollo Ac Ciad
Viruses | Year: 2015

Porcine reproductive and respiratory syndrome virus (PRRSV) is currently one of the most important viruses affecting the swine industry worldwide. Despite the large number of papers published each year, the participation of non-structural proteins (nsps) in the immune response is not completely clear. nsps have been involved in the host innate immune response, specifically, nsp1α/β, nsp2, nsp4 and nsp11 have been associated with the immunomodulation capability of the virus. To date, only participation by nsp1, nsp2, nsp4 and nsp7 in the humoral immune response has been reported, with the role of other nsps being overlooked. Furthermore, nsp1, nsp2, nsp5, nsp7 nsp9, nsp10, nsp11 have been implicated in the induction of IFN-γ and probably in the development of the cell-mediated immune response. This review discusses recent reports involving the participation of nsps in the modulation of the innate immune response and their role in the induction of both the humoral and cellular immune responses. © 2015 by the authors; licensee MDPI, Basel, Switzerland.

Mazorra-Manzano M.A.,Research Center En Alimentacion sarrollo Ac Ciad | Tanaka T.,University of Saskatchewan | Dee D.R.,University of Guelph | Yada R.Y.,University of Guelph
Phytochemistry | Year: 2010

Aspartic proteinases (APs) are involved in several physiological processes in plants, including protein processing, senescence, and stress response and share many structural and functional features with mammalian and microbial APs. The heterodimeric aspartic proteinase A1 from Arabidopsis thaliana (AtAP A1) was the first acid protease identified in this model plant, however, little information exists regarding its structure function characteristics. Circular dichroism analysis indicated that recombinant AtAP A1 contained an higher α-helical content than most APs which was attributed to the presence of a sequence known as the plant specific insert in the mature enzyme. rAtAP A1 was stable over a broad pH range (pH 3-8) with the highest stability at pH 5-6, where 70-80% of the activity was retained after 1 month at 37 °C. Using calorimetry, a melting point of 79.6 °C was observed at pH 5.3. Cleavage profiles of insulin β-chain indicated that the enzyme exhibited a higher specificity as compared to other plant APs, with a high preference for the Leu15-Tyr16 peptide bond. Molecular modeling of AtAP A1 indicated that exposed histidine residues and their interaction with nearby charged groups may explain the pH stability of rAtAP A1. © 2009 Elsevier Ltd. All rights reserved.

Rodriguez-Figueroa J.C.,Research Center En Alimentacion sarrollo Ac Ciad | Reyes-Diaz R.,Research Center En Alimentacion sarrollo Ac Ciad | Gonzalez-Cordova A.F.,Research Center En Alimentacion sarrollo Ac Ciad | Troncoso-Rojas R.,Research Center En Alimentacion sarrollo Ac Ciad | And 2 more authors.
Journal of Dairy Science | Year: 2010

Angiotensin I-converting enzyme inhibitory (ACEI) activity was evaluated and compared in <3KDa water-soluble extracts (WSE) isolated from milk fermented by wild and commercial starter culture Lactococcus lactis strains after 48h of incubation. The highest ACEI activities were found in WSE from milk inoculated with wild L. lactis strains isolated from artisanal dairy products and commercial starter cultures. On the other hand, the lowest ACEI activities were found in WSE from milk inoculated with wild strains isolated from vegetables. Moreover, the IC50 values (concentration that inhibits 50% activity) of WSE from artisanal dairy products were the lowest, indicating that these fractions were the most effective in inhibiting 50% of ACE activity. In fact, a strain isolated from artisanal cheese presented the lowest IC50 (13μg/mL). Thus, it appears that wild L. lactis strains isolated from artisanal dairy products and commercial starter cultures showed good potential for the production of fermented dairy products with ACEI properties. © 2010 American Dairy Science Association.

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