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Jimenez-Gutierrez L.R.,Research Center en Alimentacion sarrollo | Uribe-Carvajal S.,National Autonomous University of Mexico | Chimeo C.,Research Center en Alimentacion sarrollo | Muhlia-Almazan A.,Research Center en Alimentacion sarrollo
Journal of Bioenergetics and Biomembranes | Year: 2014

Cytochrome c oxidase (COX), which is located in the inner membrane of mitochondria, is a key constituent of the electron transport chain that catalyzes the reduction of oxygen. The Pacific whiteleg shrimp Litopenaeus vannamei is constantly exposed to hypoxic conditions, which affects both the central metabolism and the mitochondrial function. The purpose of this study was to isolate shrimp mitochondria, identify the COX complex and to evaluate the effect of hypoxia on the shrimp mitochondrial function and in the COX activity. A 190 kDa protein was identified as COX by immunodetection techniques. The effect of hypoxia was confirmed by an increase in the shrimp plasma L-lactate concentration. COX activity, mitochondrial oxygen uptake and protein content were reduced under hypoxic conditions, and gradually restored as hypoxia continued, this suggests an adaptive mitochondrial response and a highly effective COX enzyme. Both mitochondrial oxygen uptake and COX activity were completely inhibited by KCN and sodium azide, suggesting that COX is the unique oxidase in L. vannamei mitochondria. © 2013 Springer Science+Business Media.


Martinez-Cruz O.,Research Center en Alimentacion sarrollo | Varela-Romero A.,University of Sonora | Muhlia-Almazan A.,Research Center en Alimentacion sarrollo
Journal of Bioenergetics and Biomembranes | Year: 2011

In the mitochondrial FOF1 ATP-synthase/ATPase complex, subunits α and β are part of the extrinsic portion that catalyses ATP synthesis. Since there are no reports about genes and proteins from these subunits in crustaceans, we analyzed the cDNA sequences of both subunits in the whiteleg shrimp Litopenaeus vannamei and their phylogenetic relationships. We also investigated the effect of hypoxia on shrimp by measuring changes in the mRNA amounts of atpα and atpβ. Our results confirmed highly conserved regions for both subunits and underlined unique features among others. The ATPβ deduced protein of shrimp was less conserved in size and sequence than ATPα. The relative mRNA amounts of atpα and atpβ changed in shrimp pleopods; hypoxia at 1.5 mg/L caused an increase in atpβ transcripts and a subsequent decrease when shrimp were re-oxygenated. Results confirm that changes in the mRNAs of the ATP-synthase subunits are part of the mechanisms allowing shrimp to deal with the metabolic adjustment displayed to tolerate hypoxia. © 2011 Springer Science+Business Media, LLC.


Dorantes-Aranda J.J.,National Autonomous University of Mexico | Dorantes-Aranda J.J.,University of Tasmania | Garcia-De La Parra L.M.,Research Center en Alimentacion sarrollo | Alonso-Rodriguez R.,National Autonomous University of Mexico
Environmental Toxicology | Year: 2010

The dinoflagellate Cochlodinium polykrikoides isolated from Bahía de La Paz, Gulf of California, showed an important short-term toxic effect on the spotted rose snapper Lutjanus guttatus. This microalga was able to decrease fish liver catalase activity and lipid peroxidation. Fish exposed to live dinoflagellates developed an abnormal mucus secretion on the gills that was directly related to algal cell concentration. Hepatic catalase inhibition and an increase in mucus secretion on the gills occurred when fish were exposed to 2 × 106 cells L-1 of C. polykrikoides. Lipid peroxidation was significantly different at 4 × 106 cells L-1 and the hepatosomatic index decreased at 3 × 106 cells L-1. Our results suggest that oxidative stress contributes, at least in part, to the ichthyotoxic effect of C. polykrikoides from the Gulf of California. © 2009 Wiley Periodicals, Inc.


PubMed | Research Center En Alimentacion sarrollo
Type: Journal Article | Journal: International journal of food sciences and nutrition | Year: 2015

Phytoestrogens have generated interest in human health in view of their potential effect to reduce the risk of developing chronic diseases. Serum levels of phytoestrogens have been proposed as an alternative to measure the exposure of phytoestrogens. We evaluated the use of serum as a biomarker of phytoestrogens intake in healthy women. Phytoestrogens in serum (luteolin, kaempferol, equol, biochanin A, formononetin, quercetin, naringenin, coumestrol, secoisolariciresinol, genistein, matairesinol, enterolactone, enterodiol, daidzein, glycitein and resveratrol) were analyzed by HPLC-ESI-MS. Subjects were asked to recall all foods and beverages consumed the previous 24 h. Association of dietary intake and serum concentrations was performed by Spearman correlation. Correlations were found for naringenin (r=0.47, p<0.001), luteolin (r=0.4 p<0.001), genistein (r=0.32, p<0.01) enterolactone (r=0.35, p=0.0553), coumestrol (r=0.26, p=0.0835) and resveratrol (r=0.29, p=0.0517). Serum levels as biomarkers of intake along with a 24-h recall would be useful in order to investigate the relationship between phytoestrogens and health.


PubMed | Arizona Cancer Center, Research Center En Alimentacion sarrollo, University of Alabama at Birmingham, University of Arizona and Yeshiva University
Type: Journal Article | Journal: Obesity (Silver Spring, Md.) | Year: 2016

Studies evaluating the relationship between body mass index (BMI) and mortality demonstrate a U-shaped association. To expand, this study evaluated the relationship between adiposity indices, a body shape index (ABSI) and body adiposity index (BAI), and mortality in 77,505 postmenopausal women.A prospective cohort analysis was conducted in the Womens Health Initiative to ascertain the independent relationships between adiposity indices and mortality in order to inform on the clinical usefulness of alternate measures of mortality risk. ABSI (waist circumference (cm)/[BMI(2/3) height (cm)(1/2) ]), BAI (hip circumference (cm)/[height (m)(1.5) ] - 18), weight, BMI, and waist circumference (WC) were evaluated in relation to mortality risk using adjusted Cox proportional hazards regression models.ABSI showed a linear association with mortality (HR, 1.37; 95% CI, 1.28-1.47 for quintile 5 vs. 1) while BMI and BAI had U-shaped relationships with HR of 1.30; 95% CI, 1.20-1.40 for obesity II/III BMI and 1.06, 95% CI, 0.99-1.13 for BAI. Higher WC (HR, 1.21; 95% CI, 1.13-1.29 for quintile 5 vs. 1) showed relationships similar to BMI.ABSI appears to be a clinically useful measure for estimating mortality risk, perhaps more so than BAI and BMI in postmenopausal women.


Valenzuela-Martinez C.,University of Nebraska - Lincoln | Pena-Ramos A.,Research Center en Alimentacion sarrollo | Juneja V.K.,U.S. Department of Agriculture | Korasapati N.R.,University of Nebraska - Lincoln | And 2 more authors.
Journal of Food Protection | Year: 2010

Inhibition of Clostridium perfringens spore germination and outgrowth in ground turkey roast containing minimal ingredients (salt and sugar), by buffered vinegar (MOstatin V) and a blend (buffered) of lemon juice concentrate and vinegar (MOstatin LV) was evaluated. Ground turkey roast was formulated to contain sea salt (1.5%), turbinado sugar (0.5%), and various concentrations of MOstatin V (0.75, 1.25, or 2.5%) or MOstatin LV (1.5, 2.5, or 3.5%), along with a control (without MOstatins). The product was inoculated with a three-strain spore cocktail of C. perfringens to obtain initial spore levels of ca. 2.0 to 0.5 log CFU/g. Inoculated products were vacuum packaged, heat shocked for 20 min at 75°C, and cooled exponentially from 54.4 to 4.0°C in 6.5, 9, 12, 15, 18, or 21 h. In control samples without MOstatin V or MOstatin LV, C. perfringens populations reached 2.98, 4.50, 5.78, 7.05, 7.88, and 8.19 log CFU/g (corresponding increases of 0.51, 2.29, 3.51, 4.79, 5.55, and 5.93 log CFU/g) in 6.5, 9, 12, 15, 18, and 21 h of chilling, respectively. MOstatin V (2.5%) and MOstatin LV (3.5%) were effective in inhibiting C. perfringens spore germination and outgrowth in ground turkey roast to <1.0 log CFU/g during abusive chilling of the product within 21 h. Buffered vinegar and a blend (buffered) of lemon juice concentrate and vinegar were effective in controlling germination and outgrowth of C. perfringens spores in turkey roast containing minimal ingredients.


Mas J.F.,National Autonomous University of Mexico | Vega A.P.,University of Guanajuato | Reyes A.A.,Research Center en Alimentacion sarrollo | Santiago M.A.C.,Colegio de Mexico | Sandoval A.F.,National Autonomous University of Mexico
International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences - ISPRS Archives | Year: 2015

In order to identify drivers of land use / land cover change (LUCC), the rate of change is often compared with environmental and socio-economic variables such as slope, soil suitability or population density. Socio-economic information is obtained from census data which are collected for individual households but are commonly presented in aggregate on the basis of geographical units as municipalities. However, a common problem, known as the modifiable areal unit problem (MAUP), is that the results of statistical analysis are not independent of the scale and the spatial configuration of the units used to aggregate the information. In this article, we evaluate how strong MAUP effects are for a study on the deforestation drivers in Mexico at municipality level. This was done by taking socio-economic variables from the 2010 Census of Mexico along with environmental variables and the rate of deforestation. As population census is given for each human settlement and environmental variables are obtained from high resolution spatial database, it was possible to aggregate the information using spatial units ("pseudo municipalities") with different sizes in order to observe the effect of scale and aggregation on the values of bivariate correlations (Pearsons r) between pairs of variables. We found that MAUP produces variations in the results, and we observed some variable pairs and some configurations of the spatial units where the effect was substantial.


Vallejo-Cordoba B.,Research Center en Alimentacion sarrollo | Gonzalez-Cordova A.F.,Research Center en Alimentacion sarrollo
Electrophoresis | Year: 2010

This review presents an overview of the applicability of CE in the analysis of chemical and biological contaminants involved in emerging food safety issues. Additionally, CE-based genetic analyzers' usefulness as a unique tool in food traceability verification systems was presented. First, analytical approaches for the determination of melamine and specific food allergens in different foods were discussed. Second, natural toxin analysis by CE was updated from the last review reported in 2008. Finally, the analysis of prion proteins associated with the "mad cow" crises and the application of CE-based genetic analyzers for meat traceability were summarized. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.


Pfeiler E.,Research Center en Alimentacion sarrollo | Johnson S.,University of California at San Diego | Marrow T.A.,University of California at San Diego
Journal of the Lepidopterists' Society | Year: 2012

Nucleotide sequence data from a segment of the mitochondrial cytochrome c oxidase subunit I (COI) gene, known as the barcode segment, were used to examine phylogenetic relationships and systematics of buckeye butterflies (Nymphalidae: Nymphalinae: Junonia) from the New World, with emphasis on taxa from western North America Three nominal species have been recognized for North America, J., evarete (Cramer) ,J. genoveva (Cramer), and J., coenia Hübner, with additional species recently proposed for the West Indies and northern South America The distinctive Andean buckeye , J ., vestina C. Felder & R. Felder, along with J. evarete and J., genoveva, are also components of the South American fauna With the exception ofJ., vestina, butterflies comprising the New World Junonia have had a confused taxonomie history, and species assignments are often problematic. Our results show that the barcode segment resolves the two major clades of New World Junonia, referred to here as clades A and B, with similar high support seen in an earlier phylogenetic study using both mitochondrial and nuclear genes. Within clade A ,J. vestina resolved in a basal position toJ., evarete from South America and the Caribbean. The data further suggest that species assignments in some populations of New World Junonia clustering in clade B (J. coenia + J. genoveva) need to be reevaluated. DNA barcodes, although failing to resolve all recognized species and subspecies level taxa of New World Junonia, probably owing to relatively recent divergences, can provide valuable tools for identifying the two major lineages, and when used in conjunction with morphological, ecological, behavioral and life history information can provide insights into the taxonomy and evolution of this difficult group.


Flores D.I.,Research Center Cientifica Educacion Superior Of Ensenada | Sotelo-Mundo R.R.,Research Center en Alimentacion sarrollo | Brizuela C.A.,Research Center Cientifica Educacion Superior Of Ensenada
PLoS ONE | Year: 2014

The automatic identification of catalytic residues still remains an important challenge in structural bioinformatics. Sequence-based methods are good alternatives when the query shares a high percentage of identity with a well-annotated enzyme. However, when the homology is not apparent, which occurs with many structures from the structural genome initiative, structural information should be exploited. A local structural comparison is preferred to a global structural comparison when predicting functional residues. CMASA is a recently proposed method for predicting catalytic residues based on a local structure comparison. The method achieves high accuracy and a high value for the Matthews correlation coefficient. However, point substitutions or a lack of relevant data strongly affect the performance of the method. In the present study, we propose a simple extension to the CMASA method to overcome this difficulty. Extensive computational experiments are shown as proof of concept instances, as well as for a few real cases. The results show that the extension performs well when the catalytic site contains mutated residues or when some residues are missing. The proposed modification could correctly predict the catalytic residues of a mutant thymidylate synthase, 1EVF. It also successfully predicted the catalytic residues for 3HRC despite the lack of information for a relevant side chain atom in the PDB file. © 2014 Flores et al.

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