Research Center cnologia Animal Institute Valenciano Of Investigaciones Agrarias Cita Ivia

Segorbe, Spain

Research Center cnologia Animal Institute Valenciano Of Investigaciones Agrarias Cita Ivia

Segorbe, Spain
SEARCH FILTERS
Time filter
Source Type

Gomez-Fernandez J.,Leon Institute of Technology | Gomez-Izquierdo E.,Leon Institute of Technology | Tomas C.,Research Center cnologia Animal Institute Valenciano Of Investigaciones Agrarias Cita Ivia | Moce E.,Research Center cnologia Animal Institute Valenciano Of Investigaciones Agrarias Cita Ivia | De Mercado E.,Leon Institute of Technology
Reproduction in Domestic Animals | Year: 2013

The aim of the present study was to determine whether the levels of reactive oxygen species (ROS) substances production and the levels of lipid peroxidation of the sperm membrane were related to the quality that the ejaculates exhibited after cryopreservation in boars. Ejaculates from 42 healthy boars were used in this study and they were cryopreserved with the lactose-egg yolk extender (LEY). Several sperm quality parameters were assessed by flow cytometry in samples incubated for 30 and 150min at 37°C after thawing: the percentage of sperm with intact plasma membrane (SIPM), intracellular reactive oxygen substances production through mean of DCF fluorescence intensity of total sperm (mean-DCF) and the percentage of viable and non-viable sperm containing oxidized BODIPY (VSOB and NVSOB). In addition, the percentages of total motile (TMS) and progressively motile sperm (PMS) were assessed at the same incubation times with a computer-assisted sperm analysis system. The classification of the ejaculates into good or bad freezers was performed through hierarchical cluster analysis from SIPM and TMS at 150min post-thawing. The ejaculates of those males classified as good freezers exhibited higher (p<0.05) SPIM, TMS and PMS than the bad freezers, although both groups presented similar (p>0.05) VSOB, NVSOB and mean-DCF. Therefore, these results show that lipid peroxidation and the amount of reactive oxygen substances in the sperm after cryopreservation are similar between boars classified as good or bad freezers. © 2012 Blackwell Verlag GmbH.


Gomez-Fernandez J.,Leon Institute of Technology | Gomez-Izquierdo E.,Leon Institute of Technology | Tomas C.,Research Center cnologia Animal Institute Valenciano Of Investigaciones Agrarias Cita Ivia | Moce E.,Research Center cnologia Animal Institute Valenciano Of Investigaciones Agrarias Cita Ivia | De Mercado E.,Leon Institute of Technology
Animal Reproduction Science | Year: 2012

The aim of the present study was to evaluate the cryoprotectant effect of different non-permeating sugars for boar sperm. Pooled semen from three boars was used for the experiments. In the first experiment, the sperm quality of boar sperm cryopreserved with an egg-yolk based extender supplemented with different monosaccharides (glucose, galactose or fructose) was compared to a control cryopreserved in lactose-egg yolk extender. In the second experiment, the effect of five disaccharides (lactose, sucrose, lactulose, trehalose or melibiose) on boar sperm cryosurvival was studied. Several sperm quality parameters were assessed by flow cytometry in samples incubated for 30 and 150. min at 37. °C after thawing: percentages of sperm with intact plasma membrane (SIPM), sperm presenting high plasma membrane fluidity (HPMF), sperm with intracellular reactive oxygen substances production (IROSP) and apoptotic sperm (AS). In addition, the percentages of total motile (TMS) and progressively motile sperm (PMS) were assessed at the same incubation times with a computer-assisted sperm analysis system. Freezing extenders supplemented with each of the monosaccharide presented smaller cryoprotective effect than the control extender supplemented with lactose (P<. 0.05). However, from the three monosaccharides tested, glucose provided the best sperm quality after freezing-thawing. With respect to the disaccharides studied, samples frozen with the extender supplemented with lactulose exhibited in general the lowest sperm quality, except for the percentage of capacitated sperm, which was highest (P<. 0.05) in the samples cryopreserved with the trehalose extender. Our results suggest that disaccharides have higher cryoprotective effect than monosaccharides, although the monosaccharide composition of the disaccharides is also important, since the best results were obtained with those disaccharides presenting glucose in their composition. © 2012 Elsevier B.V..


Tomas C.,Research Center cnologia Animal Institute Valenciano Of Investigaciones Agrarias Cita Ivia | Gomez-Fernandez J.,Leon Institute of Technology | Gomez-Izquierdo E.,Leon Institute of Technology | De Mercado E.,Leon Institute of Technology
Animal Reproduction Science | Year: 2014

The aim of the present study was to evaluate the effect of the holding time at 15. °C prior to cryopreservation (2, 4 and 8. h), thawing rate (37. °C for 20. s or 70. °C for 8. s) and post-thaw incubation temperature (15. °C or 37. °C) on the post-thaw boar sperm quality. These are important time periods in the freezing-thawing process which have been less studied. Sperm-rich ejaculate fractions from three healthy boars were collected once a week for five consecutive weeks and were cryopreserved with the lactose-egg yolk extender (LEY). Sperm quality was determined by assessing the motility, the acrosome status, and the sperm plasma membrane integrity at 30, 150 and 240. min of incubation. The results show that with the holding time at 15. °C prior to cryopreservation there was not a clear effect until at least 24. h of holding time. The thawing rate and the post-thaw incubation temperature, however, had a marked effect on sperm quality. When the samples were thawed at 70. °C for 8. s, the sperm viability, motility and some kinetic variables (VCL, VSL, VAP and ALH) were greater than with results observed when the samples were thawed at 37. °C for 20. s. In addition after thawing the sperm samples incubated at 15. °C had a sustained sperm quality for longer, up to 4. h post-thawing. © 2014 Elsevier B.V.

Loading Research Center cnologia Animal Institute Valenciano Of Investigaciones Agrarias Cita Ivia collaborators
Loading Research Center cnologia Animal Institute Valenciano Of Investigaciones Agrarias Cita Ivia collaborators