Research Center cnologia Agroalimentaria Of Aragon

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Research Center cnologia Agroalimentaria Of Aragon

Spain
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Gonzalez V.,Research Center cnologia Agroalimentaria Of Aragon | Garces-Claver A.,Research Center cnologia Agroalimentaria Of Aragon
Plant Disease | Year: 2017

Rosemary (Rosmarinus officinalis L.) (Labiatae) is an evergreen, woody shrub natural to the Mediterranean basin, and is widely cultivated for ornamental and medicinal purposes. In winter of 2016, garden beds in a shopping center of Zaragoza (northeast Spain) planted with 6-year-old rosemary plants (both semierect and prostrate cultivars) exhibited up to 60% of plants diseased with clear symptoms of plant stunting, reflected in a progressive drying of loose branches and eventually the collapse of the whole plant. Infected individuals did not respond to fertilization and were uprooted. At the root level, there was little development with presence of blackish secondary roots and the central core showing a grayish to rusty-brown coloration. Progress of symptoms was seen in both upright and prostrate cultivars. To isolate the responsible fungus, leaf, stem and root fragments from five plants were taken from the diseased tissues, surface sterilized for 30 s in 70% ethanol, 3 min 5% NaCl, rinsed in sterile water, and then placed on potato dextrose agar (PDA, Difco). Isolates obtained from all root fragments exhibited the typical morphology described for the so-called binucleate Rhizoctonia taxa, such as their light to dark brown, radially zonate colonies, hyphal branching pattern, growth rate, lack of any reproductive structures, and the presence of loose clusters of monilioid cells forming sclerotium-like structures of 1 to 2.2 mm diameter (Roberts 1999). ITS region of the fungus was amplified using the primers ITS1/ITS4 and sequenced (GenBank Accession No. KY624588). BLASTn comparison (Altschul et al. 1997) of the 630-bp product obtained showed a 98% similarity with several sequences of Ceratobasidium/Rhizoctonia sp. AG-G (e.g., JF519837). To confirm identification, ITS sequence was introduced in a multiple alignment including sequences from most AG testers of the Ceratobasidiaceae (multi- and binucleate species). After performing a maximum-likelihood phylogeny of these sequences, isolates from rosemary plants were determined to belong to Rhizoctonia fragariae (Ceratorhiza fragariae AG-G). For pathogenicity tests, fungal inoculum was prepared by colonizing wheat grains previously autoclaved for 3 h and grown in petri dishes for 10 days at 25°C in the dark. Eighteen one-year rosemary plants (12 infested with R. fragariae, and three positive and three negative controls), were grown in 0.2-liter pots with an autoclaved substrate of moss peat and vermiculite (3:1), and treated with colonized wheat grains at 5 g/liter. Plants were covered with plastic bags and cultured in a growth chamber at 25°C in a 12-h photoperiod for 20 days. After 12 to 15 days, symptoms similar to those reported from diseased gardening plants were observed in the test group, and binucleate Rhizoctonia were reisolated from all these plants. Controls remained healthy and asymptomatic. Although Rhizoctonia solani has been previously reported on R. officinalis in other continents including Europe (Garibaldi et al. 2013), to our knowledge this is the first report of a root rot caused by a pathogenic binucleate Rhizoctonia species on rosemary. This fungus is associated with black root rot of strawberry (Martin 1988), and its presence on R. officinalis should be checked due to the shrub’s importance both as an ornamental and natural taxon worldwide. © 2017, American Phytopathological Society. All rights reserved.


Sanz A.,University of Zaragoza | Ordovas L.,University of Zaragoza | Zaragoza P.,University of Zaragoza | Sanz A.,Research Center cnologia Agroalimentaria Of Aragon | And 2 more authors.
Meat Science | Year: 2012

Controlling meat traceability using SNPs is an effective method of ensuring food safety. We have analyzed several SNPs to create a panel for bovine genetic identification and traceability studies. One of these was the transversion g.329C. > T (Genbank accession no. AJ496781) on the cytochrome P450 17A1 gene, which has been included in previously published panels. Using minisequencing reactions, we have tested 701 samples belonging to eight Spanish cattle breeds. Surprisingly, an excess of heterozygotes was detected, implying an extreme departure from Hardy-Weinberg equilibrium (P<0.001). By alignment analysis and sequencing, we detected that the g.329C. > T SNP is a false positive polymorphism, which allows us to explain the inflated heterozygotic value. We recommend that this ambiguous SNP, as well as other polymorphisms located in this region, should not be used in identification, traceability or disease association studies. Annotation of these false SNPs should improve association studies and avoid misinterpretations. © 2012 Elsevier Ltd.


Panea B.,Research Center cnologia Agroalimentaria Of Aragon | Catalan A.,Research Center cnologia Agroalimentaria Of Aragon | Olleta J.L.,University of Zaragoza
ITEA Informacion Tecnica Economica Agraria | Year: 2010

Thirteen animals from two different genotypes, Holstein (dairy) and Pirenaica (beef), were used to study breed and endpoint temperature effects on some meat textural characteristics. Steaks from L. thoracis were randomly distributed into raw, 55°C and 70°C of internal temperature treatments, cooked in a water bath and tested. Shear force, stress and toughness in the Warner-Bratzler test and maximum compression load, stress at 20% and stress at 80% in compression test were recorded. Statistical analysis consisted on a GLM procedure with main effects and Pearson correlations between variables. Breed is an important factor on meat texture characteristics, but endpoint temperature was more important than breed. There were no interactions between main effects. Meat from the dairy breed was tougher than meat from beef breed. The increase of toughness with the increase of temperature was no linear, being greater in raw to 55°C step than in 55°C-70°C phase.


Van Opstal J.D.,Utah State University | Neale C.M.U.,Utah State University | Neale C.M.U.,University of Nebraska - Lincoln | Lecina S.,Research Center cnologia Agroalimentaria Of Aragon
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2014

Irrigation system modelling is often used to aid decision-makers in the agricultural sector. It gives insight on the consequences of potential management and infrastructure changes. However, simulating an irrigation district requires a considerable amount of input data to properly represent the system, which is not easily acquired or available. During the simulation process, several assumptions have to be made and the calibration is usually performed only with flow measurements. The advancement of estimating evapotranspiration (ET) using remote sensing is a welcome asset for irrigation system modelling. Remotely-sensed ET can be used to improve the model accuracy in simulating the water balance and the crop production. This study makes use of the Ador-Simulation irrigation system model, which simulates water flows in irrigation districts in both the canal infrastructure and on-field. ET is estimated using an energy balance model, namely SEBAL, which has been proven to function well for agricultural areas. The seasonal ET by the Ador model and the ET from SEBAL are compared. These results determine sub-command areas, which perform well under current assumptions or, conversely, areas that need re-evaluation of assumptions and a re-run of the model. Using a combined approach of the Ador irrigation system model and remote sensing outputs from SEBAL, gives great insights during the modelling process and can accelerate the process. Additionally cost-savings and time-savings are apparent due to the decrease in input data required for simulating large-scale irrigation areas. © 2014 SPIE.


Blanco M.,Research Center cnologia Agroalimentaria Of Aragon | Joy M.,Research Center cnologia Agroalimentaria Of Aragon | Alberti P.,Research Center cnologia Agroalimentaria Of Aragon | Ripoll G.,Research Center cnologia Agroalimentaria Of Aragon | Casasus I.,Research Center cnologia Agroalimentaria Of Aragon
Italian Journal of Animal Science | Year: 2014

This paper studies the performance and carcass quality of Parda de Montaña cattle under different management systems to find alternatives to concentrate feed indoor beef production. Treatments were: i) Control, with 8 bulls (216±34.3 kg initial weight; 282±45.6 days) fed concentrate and straw ad libitum during winter housing period until reaching 500 kg; ii) G-supp, with 8 steers (204±31.2 kg initial weight; 271±47.5 days) fed a total mixed ration (TMR) (50% alfalfa hay, 10% straw, 40% corn) ad libitum during winter housing period (from mid-April steers rotationally grazed on a mountain meadow supplemented with 1.8 kg dry matter corn/d until reaching 500 kg); iii) TMR, with 8 steers (200±42.5 kg initial weight; 261±39.0 days) managed as G-supp steers until mid-July, when they were housed and fed TMR ad libitum until reaching 500 kg. Control bulls had 45% greater weight gain than TMR and G-supp steers during housing period (P<0.001). In the finishing period, TMR had 31% greater weight gain than steers finished on pasture (P<0.01). At slaughter, Controls were 97-127 days younger than others (P<0.001). Steers finished on TMR had worse conformed carcasses, greater fat and fewer edible meat proportions than G-supp and Control (P<0.01). Total cost of TMR and G-supp was greater than Control, with a similar income for G-supp and Control. TMR steers were paid less because of their worse carcass quality. Hence, finishing of steers on pasture with a supplement can be a feasible alternative to fattening bulls on concentrates, depending on the relative availability and price of feedstuff. ©Copyright M. Blanco et al., 2014.


Socias I Company R.,Research Center cnologia Agroalimentaria Of Aragon | Kodad O.,Research Center cnologia Agroalimentaria Of Aragon | Fernandez I. Marti A.,Research Center cnologia Agroalimentaria Of Aragon | Alonso J.M.,Research Center cnologia Agroalimentaria Of Aragon
Collectanea Botanica | Year: 2013

The examination of the allelic diversity of the locus S in ten almond cultivars from Majorca has shown the presence of the Sf allele in four of them, as well as the presence of five new alleles non identified so far. Although the Sf allele has been described as responsible for the expression of self-compatibility in almond, it has been recently shown that this allele has two different phenotypic expressions. As a consequence, this allele cannot be always considered an index for self-compatibility of the genotypes harbouring it. The study of the four Majorcan cultivars possessing this allele has confirmed their selfincompatibility, showing that this allele is in its phenotypically active form, as it has been also observed in a group of cultivars from southern Italy, a fact possibly suggesting a connexion between these two Mediterranean almond populations.

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