Research Center Biomedica Of Oriente

Puebla de Zaragoza, Mexico

Research Center Biomedica Of Oriente

Puebla de Zaragoza, Mexico
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Alonso-Castro A.J.,National Autonomous University of Mexico | Alonso-Castro A.J.,Instituto Nacional Of Cancerologia Mexico | Villarreal M.L.,Autonomous University of the State of Morelos | Salazar-Olivo L.A.,San Luis Potosí Institute of Scientific Research and Technology | And 4 more authors.
Journal of Ethnopharmacology | Year: 2011

Aim of the study: This review provides a summary of Mexican medicinal flora in terms of ethnobotanical, pharmacology, and chemistry of natural products related to anticancer activity. Materials and methods: Bibliographic investigation was carried out by analyzing recognized books and peer-reviewed papers, consulting worldwide accepted scientific databases from the last five decades. Mexican plants with attributed anti-cancer properties were classified into six groups: (a) plant extracts that have been evaluated for cytotoxic effects, (b) plant extracts that have documented anti-tumoral effects, (c) plants with active compounds tested on cancer cell lines, (d) plants with novel active compounds found only in Mexican species, (e) plants with active compounds that have been assayed on animal models and (f) plants with anti-cancer ethnopharmacological references but without scientific studies. Results: Three hundred plant species belonging to 90 botanical families used for cancer treatment have been recorded, of which only 181 have been experimentally analyzed. The remaining 119 plant species are in use in empirical treatment of diseases consistent with cancer symptomatology. Only 88 of the plant extracts experimentally studied in in vitro cellular models have demonstrated active cytotoxic effects in at least one cancer cell line, and 14 out of the 88 have also been tested in vivo with the results that one of them demonstrated anti-neoplasic effects. A total of 187 compounds, belonging to 19 types of plant secondary metabolites, have been isolated from 51 plant extracts with active cytotoxic effects, but only 77 of these compounds (41%) have demonstrated cytoxicity. Seventeen of these active principles have not been reported in other plant species. However, only 5 compounds have been evaluated in vivo, and 3 of them could be considered as active. Conclusion: Clearly, this review indicates that it is time to increase the number of experimental studies and to begin to conduct clinical trials with those Mexican plants and its active compounds selected by in vitro and in vivo activities. Also, the mechanisms of action by which plant extracts and their active compounds exert anti-cancer effects remain to be studied. © 2010 Elsevier Ireland Ltd. All rights reserved.


Alonso-Castro A.J.,National Autonomous University of Mexico | Ortiz-Sanchez E.,National Autonomous University of Mexico | Garcia-Regalado A.,Metropolitan Autonomous University | Nunez-Martinez J.M.,Metropolitan Autonomous University | And 5 more authors.
Journal of Ethnopharmacology | Year: 2013

Ethnopharmacological relevance: Justicia spicigera is used for the empirical treatment of cervical cancer in Mexico. Recently, we showed that Justicia spicigera extracts exerted cytotoxic and antitumoral effects and the major component of this extract was kaempferitrin (KM). Materials and methods: The cytotoxic and apoptotic effect of KM on human cancer cells and human nontumorigenic cells were evaluated using MTT and TUNEL assays, and Annexin V/Propidium iodide detection by flow cytometry. The effect of KM on cell cycle was analyzed by flow cytometry with propidium iodide. The apoptotic and cell cycle effects were also evaluated by western blot analysis. Also, different doses of KM were injected intraperitoneally daily into athymic mice bearing tumors of HeLa cells during 32 days. The growth and weight of tumors were measured. Results: KM induces high cytotoxic effects in vitro and in vivo against HeLa cells. The general mechanisms by which KM induces cytotoxic effects include: cell cycle arrest in G1 phase and apoptosis via intrinsic pathway in a caspase dependent pathway. Also, KM exerts chemopreventive and antitumor effects. Conclusion: KM exerts cytotoxic and antitumor effects against HeLa cells. © 2012 Elsevier Ireland Ltd.


Alonso-Castro A.J.,National Autonomous University of Mexico | Ortiz-Sanchez E.,Division de Investigacion Basica | Dominguez F.,Research Center Biomedica Of Oriente | Lopez-Toledo G.,Division de Investigacion Basica | And 3 more authors.
Journal of Ethnopharmacology | Year: 2012

Ethnopharmagological relevance: Medicinal plants are an important source of antitumor compounds. This study evaluated the acute toxicity in mice, as well as the cytotoxic and antitumoral effects of methanolic extracts of Croton lechleri leaves (CLE). Materials and methods: The cytotoxicity of CLE on human cancer cell lines and human non-cancerous cells was evaluated using the MTT and apoptosis assays. Apoptosis induced by CLE on human cancer cell lines was determined using flow cytometry with annexin-Alexa 488/propidium iodide. The acute toxicity in mice was performed according to the Lorke procedure. Different doses of CLE were injected intraperitoneally daily into athymic mice bearing tumor during 18 days. The growth and weight of tumors was measured. Results: CLE showed low IC 50 values on HeLa (17 μg/ml) cells but lack toxic effects against human normal cells. Induction of cell death in HeLa cells by CLE was confirmed by an increase of apoptosis (Annexin/PI) by 30% compared to untreated cells. The LD 50 was 356 mg/kg by intraperitoneal route (i.p.) and 500 mg/kg by oral route. CLE administrated at 1, 10 and 50 mg/kg i.p. inhibited the tumor growth by 38%, 48% and 59%, respectively, in mice bearing HeLa tumor. Conclusion: Croton lechleri shows moderate toxic effects in vivo, exerts cytotoxic effects on HeLa cells and has antitumor effects in mice bearing HeLa tumor. © 2012 Elsevier Ireland Ltd. All rights reserved.


Anaya-Ruiz M.,Research Center Biomedica Of Oriente | Perez-Santos J.L.M.,Autonomous University of Puebla
Asian Pacific Journal of Cancer Prevention | Year: 2013

MicroRNAs (miRNAs) are small, non-coding RNAs (18-25 nucleotides) that post-transcriptionally modulate gene expression by negatively regulating the stability or translational efficiency of their target mRNAs. In this context, the present study aimed to evaluate the in vitro effects of miR-485 mimics in breast carcinoma T47D cells. Forty-eight hours after T47D cells were transfected with miR-485 mimics, an MTT (3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide) assay was utilized to determine the effects on cell viability. Colony formation and cell migration assays were adopted to determine whether miR-485 affects the proliferation rates and cell migration of breast carcinoma T47D cells.Our results showed that ectopic expression of miR-485 resulted in a significant decrease in cell growth, cell colony formation, and cell migration. These findings suggest that miR- 485 might play an important role in breast cancer by suppressing cell proliferation and migration.


PubMed | University of Guanajuato, Autonomous University of San Luis Potosi and Research Center Biomedica Of Oriente
Type: Journal Article | Journal: Drug development research | Year: 2016

Preclinical Research The aim of the present study was to evaluate the antinociceptive and sedative activity of an ethanol extract of Justicia spicigera an evergreen used in Mexican traditional medicine for the relief of pain, wounds, fever and inflammation. At 200 mg/kg po, the maximum dose examined, the ethanol extract of J. spicigera (JSE) had analgesic activity in mice in the acetic acid writhing test, the second phase of the formalin test and the tail flick test that was similar in efficacy to the NSAID, naproxen (150 mg/kg po). JSE was inactive in the hot plate test and and the ketamine-induced sleeping time test; it had no sedative effects. These results show that the ethanol extract from the leaves of J. spicigera has antinociceptive effects in mice without inducing sedation. Drug Dev Res 77 : 180-186, 2016. 2016 Wiley Periodicals, Inc.


Alonso-Castro A.J.,San Luis Potosí Institute of Scientific Research and Technology | Alonso-Castro A.J.,Instituto Nacional Of Cancerologia | Zapata-Bustos R.,San Luis Potosí Institute of Scientific Research and Technology | Dominguez F.,Research Center Biomedica Of Oriente | And 3 more authors.
Phytomedicine | Year: 2011

Some Magnolia (Magnoliaceae) species are used for the empirical treatment of diabetes mellitus, but the antidiabetic properties of Magnolia dealbata have not yet been experimentally validated. Here we report that an ethanolic extract of Magnolia dealbata seeds (MDE) and its active principles honokiol (HK) and magnolol (MG) induced the concentration-dependent 2-NBDG uptake in murine 3T3-F442A and human subcutaneous adipocytes. In insulin-sensitive adipocytes, MDE 50 μg/ml induced the 2-NBDG uptake by 30% respect to insulin, while HK and MG, 30 μM each, did it by 50% (murine) and 40% (human). The simultaneous application of HK and MG stimulated 2-NBDG uptake by 70% in hormone-sensitive cells, on which Magnolia preparations exerted synergic effects with insulin. In insulin-resistant adipocytes, MDE, HK and MG induced 2-NBDG uptake by 57%, 80% and 96% respect to Rosiglitazone (RGZ), whereas HK and MG simultaneously applied stimulated 2-NBDG uptake more efficiently than RGZ (120%) in both murine and human adipocytes. Inhibitors of the insulin-signaling pathway abolished the glucose uptake induced by Magnolia dealbata preparations, suggesting that their antidiabetic effects are mediated by this signaling pathway. In addition, MDE, HK and MG exerted only mild to moderate proadipogenic effects on 3T3-F442A and human preadipocytes, although the combined application of HK and MG markedly increased the lipid accumulation in both cell types. In summary, Magnolia dealbata and its active principles HK and MG stimulate glucose uptake in insulin-sensitive and insulin-resistant murine and human adipocytes using the insulin signaling pathway. © 2011 Elsevier GmbH. All rights reserved.


Anaya-Ruiz M.,Research Center Biomedica Of Oriente | Cebada J.,Autonomous University of Puebla | Delgado-Lopez G.,Research Center Biomedica Of Oriente | Sanchez-Vazquez M.L.,Research Center Biomedica Of Oriente | Perez-Santos J.L.M.,Autonomous University of Puebla
Asian Pacific Journal of Cancer Prevention | Year: 2013

MicroRNAs (miRNAs) are small, non-coding RNAs (18-25 nucleotides) that post-transcriptionally modulate gene expression by negatively regulating the stability or translational efficiency of their target mRNAs. In this context, the present study aimed to evaluate the in vitro effects of miR-153 inhibition in the breast carcinoma cell line MDA-MB-231. Forty-eight hours after MDA-MB-231 cells were transfected with the miR-153 inhibitor, an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was utilized to determine the effects of miR-153 on cell viability. Flow cytometry analysis and assessment of caspase 3/7 activity were adopted to determine whether miR-153 affects the proliferation rates and apoptosis levels of MDA-MB-231 cells. Our results showed that silencing of miR-153 significantly inhibited growth when compared to controls at 48 hours, reducing proliferation by 37.6%, and inducing apoptosis. Further studies are necessary to corroborate our findings and examine the potential use of this microRNA in future diagnostic and therapeutic interventions.


Cuevas-Romero S.,Swedish University of Agricultural Sciences | Cuevas-Romero S.,National Research Center Disciplinaria en Microbiologia Animal | Hernandez-Baumgarten E.,National Autonomous University of Mexico | Kennedy S.,Agri Food and Biosciences Institute of Northern Ireland | And 3 more authors.
Virus Research | Year: 2014

The persistence of porcine rubulavirus (PorPV-LPMV) in five pigs that had survived an outbreak of a natural infection was determined. After the resolution of the outbreak, each animal was housed in an isolation pen together with one sentinel pig. Approximately every 2 months thereafter one group of animals was euthanized and tissue samples taken for virological and serological analysis. Infectious virus was not isolated from any samples; antibodies to PorPV-LPMV were detected in convalescent pigs by virus neutralisation test and blocking ELISA but not in sentinel pigs. PorPV-LPMV mRNA of the nucleoprotein (NP) and phosphoprotein (P) genes was detected by a nested polymerase chain reaction (nPCR) in samples of trigeminal and optic nerves, cervical spinal cord, tonsils, salivary gland, lung and pancreas from convalescent pigs. mRNA was also detected in the midbrain, corpus callosum, or olfactory bulb in four out of five pigs by nRT-PCR, this result was confirmed by the sequencing of a 260. bp PCR product of P gene region. The highest average viral copies/μg of total RNA occurred in the olfactory bulb and pancreas tissues of convalescent pigs and midbrain, tonsil and pancreas of sentinel pigs housed with the convalescent pigs. Satellitosis and gliosis of the midbrain, olfactory bulb, corpus callosum, medulla oblongata or choroid plexus were microscopically observed in four convalescent pigs. The control pig remained negative in all tests. The results indicate that PorPV-LPMV mRNA persists and induces a durable humoral immune response in pigs that have recovered from a natural infection. After a possible reactivation of the virus, it was transmitted to sentinel pigs in contact with the convalescent pigs. © 2014 Elsevier B.V.


Alonso-Castro A.J.,National Autonomous University of Mexico | Alonso-Castro A.J.,Instituto Nacional Of Cancerologia | Dominguez F.,Research Center Biomedica Of Oriente | Garcia-Carranca A.,Instituto Nacional Of Cancerologia | Garcia-Carranca A.,National Autonomous University of Mexico
Archives of Medical Research | Year: 2013

Background and Aims: We previously showed that extracts from Phoradendron serotinum and Croton lechleri exerted invitro cytotoxic and invivo antitumor effects and that their main component was rutin (RTN; 3-rhamnosyl-glucosylquercetin). However, it is unknown whether RTN exerts invivo antitumoral effects on human colon cancer cells. The aim of this work was to evaluate the antitumor effects of RTN on a murine model. Methods: Cytotoxic effects of RTN on human cancer and non-tumorigenic cell lines were evaluated using the MTT assay. Different doses of RTN were injected intraperitoneally daily into nu/nu mice bearing tumors of SW480 colon cancer cells during 32 days. The growth and weight of tumors were measured. Serum levels of VEGF, survival time, increase in life span and toxicological effects on body weight and organ weight were also analyzed. Results: RTN showed the highest cytotoxic effects against SW480 cells (IC50= 125 μM) as compared to the other cancer cells lines and decreased, in a dose dependent manner, the tumor volume and weight of mice bearing SW480 tumor. RTN 20 mg/kg, the highest dose tested, lacked toxic effects on body weight and relative organ weight in mice, increased mean survival time by 50 days, and decreased by 55% the VEGF serum levels compared to untreated mice. Conclusions: RTN exerts invitro cytotoxic effects on SW480 cells, induces invivo antitumor effects, lacks toxic effects on mice bearing SW480 tumor and exerts antiangiogenic properties. © 2013 IMSS.


PubMed | National Research Center Disciplinaria en Microbiologia Animal, National Autonomous University of Mexico, Research Center Biomedica Of Oriente and Swedish University of Agricultural Sciences
Type: Journal Article | Journal: Virus genes | Year: 2016

Since the report of the initial outbreak of Porcine rubulavirus (PorPV) infection in pigs, only one full-length genome from 1984 (PorPV-LPMV/1984) has been characterised. To investigate the overall genetic variation, full-length gene nucleotide sequences of current PorPV isolates were obtained from different clinical cases of infected swine. Genome organisation and sequence analysis of the encoded proteins (NP, P, F, M, HN and L) revealed high sequence conservation of the NP protein and the expression of the P and V proteins in all PorPV isolates. The V protein of one isolate displayed a mutation that has been implicated to antagonise the antiviral immune responses of the host. The M protein indicated a variation in a short region that could affect the electrostatic charge and the interaction with the membrane. One PorPV isolate recovered from the lungs showed a mutation at the cleavage site (HRKKR) of the F protein that could represent an important factor to determine the tissue tropism and pathogenicity of this virus. The HN protein showed high sequence identity through the years (up to 2013). Additionally, a number of sequence motifs of very high amino acid conservation among the PorPV isolates important for polymerase activity of the L protein have been identified. In summary, genetic comparisons and phylogenetic analyses indicated that three different genetic variants of PorPV are currently spreading within the swine population, and a new generation of circulating virus with different characteristics has begun to emerge.

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