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Jimenez-Arellanes A.,Hospital Of Especialidades | Cornejo-Garrido J.,Hospital Of Especialidades | Rojas-Bribiesca G.,Research Center Biomedica del Sur | Nicasio-Torres M.D.P.,Research Center Biomedica del Sur | And 6 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2012

Rubus liebmannii is an endemic species from Mexico used in traditional medicine primarily to treat dysentery and cough. The in vitro activity against Giardia lamblia and Entamoeba histolytica that produces the ethanolic extract of the aerial parts of the plant led us to expand the pharmacological and phytochemical research of this species. Gastrointestinal disorders including amebiasis remain one of the health problems that need to be addressed and it is of interest to find alternatives that improve their treatment. Also, it is important to emphasize that R. liebmannii grows wild in the country and is not found in abundance; therefore, alternatives that avoid overexploitation of the natural resource are mandatory. Ongoing with the evaluation of the potentialities that R. liebmannii possesses for treating infectious gastrointestinal diseases, the aim of the present study was to evaluate the biological effects and the chemical composition of the micropropagated plant. Copyright © 2012 Adelina Jiménez-Arellanes et al.


PubMed | National Autonomous University of Mexico and Research Center Biomedica Del Sur
Type: Journal Article | Journal: Veterinary parasitology | Year: 2015

Leucaena leucocephala is a tropical forage legume suggested as an alternative method to control gastrointestinal parasitism in ruminants. This study: (1) performed a bio-guided fractionation of an aqueous extract of L. leucocephala using the egg hatch assay (EHA) to identify the anthelmintic (AH)-like phytochemicals present in fresh leaves, and (2) assessed the ultrastructural damage to eggs of Cooperia spp. after incubation with the final fraction. Phytochemicals were isolated using silica gel columns and identified using high performance liquid chromatography and standards for comparison. The final fraction was evaluated using EHA at 0.06, 0.125, 0.250, 0.500 and 1.1 mg ml(-1). The lethal concentration to inhibit 50% of Cooperia spp. egg hatching (LC50) was calculated using a Probit analysis. Scanning and transmission electron microscopy revealed the ultrastructural changes present in Cooperia spp. eggs. Bio-guided isolation procedures led to the recognition of an active fraction (LlC1F3) mainly composed of quercetin (82.21%) and caffeic acid (13.42%) which inhibited 90.49 2.8% of Cooperia spp. egg hatching (P<0.05), and an LC50 of 0.06 0.14 mg ml(-1). Scanning electron microscopy (SEM) showed eggs exposed to the active fraction had an irregular external layer with small projections and ruptures of lateral eggshell walls. Transmission electron microscopy (TEM) showed changes to Cooperia spp. eggs in electro-density, including the thickness of the eggshell layers and fractures after incubation with the final fraction (LlC1F3). Changes in bioactivity after purification suggest synergistic interactions between quercetin and caffeic acid.


Lombardo-Earl G.,Metropolitan Autonomous University | Lombardo-Earl G.,Research Center Biomedica Del sur | Roman-Ramos R.,Metropolitan Autonomous University | Zamilpa A.,Research Center Biomedica Del sur | And 4 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2014

Sechium edule is traditionally used in Mexico as a therapeutic resource against renal diseases and to control high blood pressure. The purpose of this work is to evaluate the antihypertensive effect of the hydroalcoholic extract obtained from the roots of this plant, including its fractions and subfractions, on different hypertension models induced with angiotensin II (AG II). The hydroalcoholic extract was tested on an in vitro study of isolated aorta rings denuded of endothelial cells, using AG II as the agonist; this assay proved the vasorelaxant effect of this extract. Vagotomized rats were administered different doses of AG II as well as the Hydroalcoholic extract, which reduced blood pressure in 30 mmHg approximately; subsequently this extract was separated into two fractions (acetone and methanol) which were evaluated in the acute hypertension mouse model induced with AG II, where the acetone fraction was identified as the most effective one and was subsequently subfractioned using an open chromatographic column packed with silica gel. The subfractions were also evaluated in the acute hypertension model. Finally, the extract, fraction, and active subfraction were analyzed by MS-PDA-HPLC, identifying cinnamic derivative compounds like cinnamic acid methyl ester. © 2014 Galia Lombardo-Earl et al.


Leon-Rivera I.,Autonomous University of the State of Morelos | Castro J.M.,Autonomous University of the State of Morelos | Miron-Lopez G.,Autonomous University of Yucatán | Del Rio-Portilla F.,National Autonomous University of Mexico | And 8 more authors.
Journal of Natural Medicines | Year: 2014

The methanol-soluble extract from the root of Ipomoea tyrianthina was studied in order to isolate compounds with activity on the central nervous system and vasorelaxant effects. Chromatographic methods were used to isolate and purify seven new glycolipids (2-8). The structures of compounds 1-8 were elucidated by a combination of NMR spectroscopy and mass spectrometry. Tyrianthinoic acid (1) is a glycosidic acid composed of a linear pentasaccharide core bonded to a 11-hydroxyhexadecanoic acid. The structure of tyrianthinic acids III (2), IV (3), and V (4) consists of a partially acylated tyrianthinoic acid. Tyrianthinic acid VI (8) is a tetrasaccharide core bonded to a jalapinolic acid, acylated by a 2-methyl-3-hydroxybutanoic acid. Tyrianthins C (5), D (6), and E (7) are ester-type heterodimers of scammonic acid A with different acylating residues in the two monomeric units. The macrolactonization site was located at C-3 of the rhamnose unit. The position of the ester linkage for monomeric unit B on the macrocyclic unit A was established at C-4 of the terminal quinovose. Compounds 5-7 increased the sleeping time induced by pentobarbital and the release of gamma-aminobutyric acid in brain cortex. In addition, compounds 5-7 showed significant in vitro relaxant effects on aortic rat rings, in endothelium- and concentration-dependent manners. © 2014 The Japanese Society of Pharmacognosy and Springer Japan.


PubMed | Research Center Biomedica del Sur and Autonomous University of the State of Morelos
Type: Journal Article | Journal: Molecules (Basel, Switzerland) | Year: 2017

The genetically transformed hairy root line LRT 7.31 obtained by infecting leaf explants of


Leos-Rivas C.,Autonomous University of Nuevo León | Verde-Star M.J.,Autonomous University of Nuevo León | Torres L.O.,Research Center Biomedica Del sur | Oranday-Cardenas A.,Autonomous University of Nuevo León | And 4 more authors.
Journal of Medicinal Food | Year: 2011

Borage (Borago officinalis) is a plant with nutritional value that is also used in traditional medicine to treat gastrointestinal disease. This study investigated the amoebicidal activity of a methanol extract of borage. The 50% inhibitory concentration (IC 50) of the extract for Entamoeba histolytica was 33 μg/mL. The 50% lethal dose of the extract for brine shrimp was greater than 1,000 μg/mL. The IC 50 of the extract for Vero cells was 203.9 μg/mL. These results support the use of borage to prevent diseases associated with E. histolytica infection. © 2011, Mary Ann Liebert, Inc.


Ramirez-Estrada K.,University of Barcelona | Osuna L.,Research Center Biomedica Del sur | Moyano E.,University Pompeu Fabra | Bonfill M.,University of Barcelona | And 3 more authors.
Phytochemistry | Year: 2015

The response of two Taxus cell systems to the action of cyclodextrin (CD) and coronatine (CORO), supplied to the culture medium either separately or together, was studied. Two-stage Taxus globosa and Taxus media cell cultures were established and the elicitors were added at the beginning of the second stage. Growth, taxane production, and the expression of known taxol biosynthetic genes, including the recently characterized CoA ligase gene, were studied. Although CORO reduced the growth capacity of both cell lines, CD apparently counteracted this negative effect. Taxane production was significantly enhanced by the simultaneous addition of CD and CORO to the medium. The total taxane production in the T. media cell line was more than double that of T. globosa, but in the latter more than 90% of the taxanes produced were excreted to the medium. Individual taxane patterns also differed: at the height of production, the main taxanes in T. globosa cultures were cephalomannine and 10-deacetyltaxol, and in T. media, taxol and baccatin III. The low transcript levels of taxane biosynthetic genes found in T. globosa cells mirrored the lower taxane production in these cultures, while a high expression was strongly correlated with a high taxane production in T. media. © 2015 Elsevier Ltd. All rights reserved.


Aldana-Llanos L.,National Polytechnic Institute of Mexico | Salinas-Sanchez D.O.,University of Central Mexico | Valeds-Estrada Ma.E.,National Polytechnic Institute of Mexico | Gutirrez-Ochoa M.,National Polytechnic Institute of Mexico | And 2 more authors.
Southwestern Entomologist | Year: 2012

The bioinsecticidal activity of organic extracts of Tagetes erecta L. on L1 larvae of fall armyworm, Spodoptera frugiperda (E. Smith), was evaluated. The acetonic extract of the leaves (500 ppm) of T. erecta exhibited an antifeedant effect, causing 50% reduction in larval weight in comparison with that of the check. Information obtained on larval weight at 7 days corroborated with results obtained at 14 days when T. erecta extracts killed most larvae. The following killed the most larvae: n-hexane leaf (48%), acetone leaf (60%), and ethanol leaf, which caused most larval mortality of 72%. T. erecta leaf extracts were toxic to 4080% of the pupae. The leaf extracts hexane, acetone, and ethanol of T. erecta killed many larvae and were evaluated at different concentrations: 125, 250, 750, and 1,000 ppm. The LC50 for n-hexano leaf extract was 312.2 ppm (X 2 = 0.1102); for acetone leaf extract of T. erecta, 246.9 ppm (X 2 = 0.1598), and for ethanol leaf extract, 152.2 ppm (X 2 = 0.1504), with a 95% confidence. Thus, use of these extracts is suggested as a more environmentally friendly alternative to combat insect pests of maize, Zea mays L.


PubMed | Research Center Biomedica del Sur
Type: Journal Article | Journal: Plant cell reports | Year: 2013

Mimosa tenuiflora (Willd.) Poiret (Leguminosae) was micropropagated throughin vitro culture of axillary buds on Murashige and Skoog (MS) medium. Shoot formation was achieved when the media were supplemented with 0.1 mg.L(-1) IAA + 3 mg.L(-1) KN.In vitro rooting of regenerated shoots was achieved when 0.1 mg.L(-1) KN was combined with 1 mg.L(-1) IBA in the absence of IAA. Ninety-four percent of the rooted plants were succesfully adapted to field conditions and grown in the soil. A total of 180 trees grown under these conditions were obtained over a one-year period.


PubMed | Research Center Biomedica del Sur
Type: Journal Article | Journal: Revista medica del Instituto Mexicano del Seguro Social | Year: 2010

To evaluate the use of complementary alternative medicine (CAM) in patients infected with the human immunodeficiency virus (HIV), and to identify the type and factors associated to its use.Cross-sectional study made in HIV clinics of three majors institutions from Morelos State, Mexico; the sample was 293 subjects; a specific questionnaire looking for demographic information and CAM use was applied.The use of CAM was 73.4%; 71% informed to the physician of their use. 51% of the patients reported using nutritional complements, 29.7% herbal products and 19% used physical agents like exercise, Chinese medicine, and therapy by contact. A total of 96.6 % of the users reported some perception of benefit with its use. The patients with low income, IMSS beneficiaries and on antirretroviral treatment were the greatest CAM users.CAM use is a common practice among HIV (+) patients. The multivitamins and herbal products were the most used. The majority was prescribed by health personnel and the users showed some benefit. Other longitudinal studies are required to establish the CAM impact on the disease evolution and the quality of life of HIV (+) patients.

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