Research Center Agraria Of Albaladejito

Chillarón de Cuenca, Spain

Research Center Agraria Of Albaladejito

Chillarón de Cuenca, Spain
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Usano-Alemany J.,University of Bonn | Pala-Paul J.,Complutense University of Madrid | Herraiz-Penalver D.,Research Center Agraria Of Albaladejito
Chemistry and Biodiversity | Year: 2014

A comprehensive description of diverse essential-oil chemical profiles was conducted with twelve individual plants of Salvia lavandulifolia VAHL (Spanish sage) gathered from wild populations and grown under identical culture conditions.Whereas the chemical polymorphism within species of aromatic plants is well known, both the statistical significance of the differences in the chemical composition and the setting of chemical profiles have been scarcely studied. Remarkably different essential-oil chemical profiles were described for the wild-growing plants, which remained different during the three years of cultivation, even when acute situations of water stress occurred. Different patterns of representative compounds throughout the phenological cycle of the species were also reported, with 1,8-cineole being the main oil compound described (contents of up to 75.2%). The Mahalanobis distance was used as a separation parameter, to assign a significance level to a single-compound chemotype. The phytochemical heterogeneity of some wild populations of Spanish sage was proved. Furthermore, some concrete genotypes presented high percentages of the following volatile compounds: limonene, 1,8-cineole, transcaryophyllene, spathulenol, and viridiflorol. © 2014 Verlag Helvetica Chimica Acta AG.


Santana P.O.,Research Center Agraria Of Albaladejito | Santana P.O.,Institute Recursos Humanos Para la Ciencia y la Tecnologia INCRECYT | Cabrera R.,University of La Laguna | Gimenez C.,University of La Laguna | And 8 more authors.
Grasas y Aceites | Year: 2012

The chemical composition and biological activities of essential oils of Salvia officinalis L., Salvia lavandulifolia Vahl., Lavandula x intermedia Emeric ex Loisel., Lavandula latifolia Medik., Lavandula angustifolia Mill. and Thymus vulgaris L. are presented. The essential oils have been analysed by Gas Chromatography Mass Spectrometry and 61 compounds were identified, 23 of which represented more than 1% of the essential oil. The 1,8 cineole (16-23%) appeared as the main compound of Salvia sp. essential oils. The high content of α-thujone was characteristic in S. officinalis oil. Remarkable concentrations of linalool (30-33%), camphor (5-17%) and linalyl acetate (9-28%) were detected in Lavandula sp. oils while carvacrol (21.6%) and p-cimene (23.7%) were the most abundant compounds in T. vulgaris oil. Biological characterization was based on their bioplaguicide activity. The essential oils studied had strong antifeedant effects against Leptinotarsa decemlineata Say, Spodoptera littoralis Boisd., Myzus persicae Sulzer and Rhopalosiphum padi L., phytotoxic activity against Lactuca sativa L. and Lolium perenne L. and also exhibited high antifungal activity against Fusarium sp. Oils from T. vulgaris and L. latifolia showed the highest levels of bioactivity against all target species. These results provide an added-value to the essential oils of aromatic plants of agro-industrial interest for its potential use in the development of natural agrochemicals.


Usano-Alemany J.,University of Bonn | Pala-Paul J.,Complutense University of Madrid | Herraiz-Penalver D.,Research Center Agraria Of Albaladejito
Biochemical Systematics and Ecology | Year: 2014

In order to clarify volatile accumulation patterns along with their adaptability to temperature stress, replicas of two different accessions of Salvia lavandulifolia appointed as chemotypes (A and B) were subjected to increasing temperatures in environmental controlled cabins. Their respective volatile chemical profiles kept their integrity until reaching the growing temperature of 24°C. Then, remarkable changes were observed. Stressed plants of both accessions showed an increase in the sesquiterpenic fraction and a decrease in the monoterpenic one, although changes in volatile percentages were highly related to the accession. Accession B overexpressed β-caryophyllene, caryophyllene oxide and manool when subjected to the higher temperature tested. On the other hand, accession A mainly showed a remarkable increase of β-bisabolene and viridiflorol. Furthermore, viridiflorol and manool have been confirmed as new chemotaxonomic markers for chemotypes A and B, respectively. The capacity to adjust the production of volatile compounds seems mostly attributed to the genetic background of the plant although more research is needed to understand the stress and its implications for the secondary metabolism. These results contribute remarkably to the chemical phenotyping of this species and may be useful for selection of genotypes. © 2014 Elsevier Ltd.


Usano-Alemany J.,Complutense University of Madrid | Herraiz-Penalver D.,Research Center Agraria Of Albaladejito | Cuadrado J.,Research Center Agraria Of Albaladejito | Diaz S.,Complutense University of Madrid | And 2 more authors.
Journal of Essential Oil-Bearing Plants | Year: 2012

Spanish sage (Salvia lavandulifolia Vahl.) is an important medicinal plant native from the Iberian Peninsula being the species of the genus most commonly grown in this area. The seasonal variation of the essential oil chemical composition in organic cropping has been analysed. Besides, the antibacterial and antifungal activity of the essential oils has been evaluated. Essential oils extracted from aerial parts of the S. lavandulifolia by steam distillation show an important variation both on the yield and on the chemical composition during the different seasons. The main constituents of the oil were identified as 1,8-cineole (21.4-33.8%), limonene (5.6-10.4%), α-pinene (10.5-17.5%), β-pinene (6.0-17.3%), myrcene (t-10.0%), camphor (6.1-9.4%), and trans-caryophyllene (4.0-8.5%), varying according to the seasons. According to our results essential oils coming from autumn and winter time produce the higher inhibition on the grow of most of the microorganism tested, and they are also more effective against Gram positive bacteria. © 2012, Har Krishan Bhalla & Sons.


Busconi M.,Catholic University of the Sacred Heart | Colli L.,Catholic University of the Sacred Heart | Sanchez R.A.,University of Castilla - La Mancha | Santaella M.,University of Castilla - La Mancha | And 6 more authors.
PLoS ONE | Year: 2015

The presence and extent of genetic variation in saffron crocus are still debated, as testified by several contradictory articles providing contrasting results about the monomorphism or less of the species. Remarkably, phenotypic variations have been frequently observed in the field, such variations are usually unstable and can change from one growing season to another. Considering that gene expression can be influenced both by genetic and epigenetic changes, epigenetics could be a plausible cause of the alternative phenotypes. In order to obtain new insights into this issue, we carried out a molecular marker analysis of 112 accessions from theWorld Saffron and Crocus Collection. The accessions were grown for at least three years in the same open field conditions. The same samples were analysed using Amplified Fragment Length Polymorphism (AFLP) and Methyl Sensitive AFLP in order to search for variation at the genetic (DNA sequence) and epigenetic (cytosine methylation) level. While the genetic variability was low (4.23% polymorphic peaks and twelve (12) effective different genotypes), the methyl sensitive analysis showed the presence of high epigenetic variability (33.57% polymorphic peaks and twenty eight (28) different effective epigenotypes). The pattern obtained by Factorial Correspondence Analysis of AFLP and, in particular, of MS-AFLP data was consistent with the geographical provenance of the accessions. Very interestingly, by focusing on Spanish accessions, it was observed that the distribution of the accessions in the Factorial Correspondence Analysis is not random but tends to reflect the geographical origin. Two clearly defined clusters grouping accessions from theWest (Toledo and Ciudad Real) and accessions from the East (Cuenca and Teruel) were clearly recognised. © 2015 Busconi et al.


Fraga B.M.,CSIC - Institute of Natural Products and Agrobiology | Diaz C.E.,CSIC - Institute of Natural Products and Agrobiology | Amador L.J.,CSIC - Institute of Natural Products and Agrobiology | Reina M.,CSIC - Institute of Natural Products and Agrobiology | And 2 more authors.
Phytochemistry | Year: 2014

A chemical study of Bethencourtia hermosae, aerial parts and in vitro root cultures, transformed by Agrobacterium rhizogenes, afforded the hitherto unreported sesquiterpenes ceratopicanol angelate (1), 8β-hydroxy-african-4(5)-en-3-one tiglate (4), 8β-hydroxy-african-4(5)-en-3-one 3′-angeloxy-2′-methylbutanoate (5), 1α,8β-dihydroxy-african-4(5)-en-3-one 8β-angelate (7) and 6α,8β-dihydroxy-african-4(5)-en-3-one 8β-angelate (8). In addition, 8β-hydroxy-african-4(5)-en-3-one (6) was isolated for the first time from a natural source, along with the rare sesquiterpenoid senecrassidiol (10) and two jacaranone derivatives 14 and 16. Known pyrrolizidine alkaloids, together with previously unreported hermosine (23), have also been isolated from this plant. The insect antifeedant activities of the extracts and compounds were studied together with their cytotoxic effects against insect (Sf9) and mammalian (CHO) cell lines. © 2014 Elsevier Ltd. All rights reserved.


PubMed | Research Center Agraria Of Albaladejito, CSIC - Institute of Agricultural Sciences and CSIC - Institute of Natural Products and Agrobiology
Type: | Journal: Phytochemistry | Year: 2014

A chemical study of Bethencourtia hermosae, aerial parts and in vitro root cultures, transformed by Agrobacterium rhizogenes, afforded the hitherto unreported sesquiterpenes ceratopicanol angelate (1), 8-hydroxy-african-4(5)-en-3-one tiglate (4), 8-hydroxy-african-4(5)-en-3-one 3-angeloxy-2-methylbutanoate (5), 1,8-dihydroxy-african-4(5)-en-3-one 8-angelate (7) and 6,8-dihydroxy-african-4(5)-en-3-one 8-angelate (8). In addition, 8-hydroxy-african-4(5)-en-3-one (6) was isolated for the first time from a natural source, along with the rare sesquiterpenoid senecrassidiol (10) and two jacaranone derivatives 14 and 16. Known pyrrolizidine alkaloids, together with previously unreported hermosine (23), have also been isolated from this plant. The insect antifeedant activities of the extracts and compounds were studied together with their cytotoxic effects against insect (Sf9) and mammalian (CHO) cell lines.


Santana-Meridas O.,Research Center Agraria Of Albaladejito | De-Los-Mozos-Pascual M.,Research Center Agraria Of Albaladejito | Fernandez J.-A.,University of Castilla - La Mancha
Acta Horticulturae | Year: 2010

As a result of implementation of the CROCUSBANK project first year, the germplasm collection of saffron and Crocus spp. allies has been created. Although it is true that is a great achievement, it is also true that we should use appropriate tools for the correct disclosure of the collection and interaction with the users. In this connection, a specific website (http://www.crocusbank.org) was designed and is being managed to provide detailed information about the project and the World Saffron and Crocus Collection.


De-Los-Mozos-Pascual M.,Research Center Agraria Of Albaladejito | Roldan M.,University of Castilla - La Mancha | Fernandez J.-A.,Campus Universitario s n
Acta Horticulturae | Year: 2010

The loss of land surface dedicated to saffron cultivation in some areas of the world, particularly in the Mediterranean Basin countries (North and South banks), has resulted in a corresponding genetic erosion of this crop. Since saffron multiplies exclusively in a vegetative way, the preservation of the presumably scarce genetic diversity is highly valuable to carry out in any breeding programme. In 2005, the European Commission launched a programme for the conservation, characterisation, collection and utilisation of genetic resources in agriculture, AGRI GEN RES. A consortium of 14 groups of 9 countries, lead by the University of Castilla-La Mancha (Spain), presented the four-years project "Genetic Resources of Saffron and Allies (Crocus spp.): CROCUSBANK". The objective of this action is to create, characterise and exploit a worldwide germplasm collection in saffron and relatives. The mother-plant collection is located in the Bank of Plant Germplasm of Cuenca (CIA Albadalejito, Spain), where 384 accessions of saffron and wild crocuses are preserved, multiplied and partially characterised at the moment. In this paper, the curator of the collection, the manager and the coordinator of the CROCUSBANK Project, present the results in this field, after two years of activity.


PubMed | University of Castilla - La Mancha, Research Center Agraria Of Albaladejito and Catholic University of the Sacred Heart
Type: Journal Article | Journal: PloS one | Year: 2015

The presence and extent of genetic variation in saffron crocus are still debated, as testified by several contradictory articles providing contrasting results about the monomorphism or less of the species. Remarkably, phenotypic variations have been frequently observed in the field, such variations are usually unstable and can change from one growing season to another. Considering that gene expression can be influenced both by genetic and epigenetic changes, epigenetics could be a plausible cause of the alternative phenotypes. In order to obtain new insights into this issue, we carried out a molecular marker analysis of 112 accessions from the World Saffron and Crocus Collection. The accessions were grown for at least three years in the same open field conditions. The same samples were analysed using Amplified Fragment Length Polymorphism (AFLP) and Methyl Sensitive AFLP in order to search for variation at the genetic (DNA sequence) and epigenetic (cytosine methylation) level. While the genetic variability was low (4.23% polymorphic peaks and twelve (12) effective different genotypes), the methyl sensitive analysis showed the presence of high epigenetic variability (33.57% polymorphic peaks and twenty eight (28) different effective epigenotypes). The pattern obtained by Factorial Correspondence Analysis of AFLP and, in particular, of MS-AFLP data was consistent with the geographical provenance of the accessions. Very interestingly, by focusing on Spanish accessions, it was observed that the distribution of the accessions in the Factorial Correspondence Analysis is not random but tends to reflect the geographical origin. Two clearly defined clusters grouping accessions from the West (Toledo and Ciudad Real) and accessions from the East (Cuenca and Teruel) were clearly recognised.

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