Research Center Agraria

Quintanar de la Orden, Spain

Research Center Agraria

Quintanar de la Orden, Spain
SEARCH FILTERS
Time filter
Source Type

Tormo M.A.,University of Extremadura | Campillo J.E.,University of Extremadura | Vina J.,University of Valencia | Go Mez-Encinas J.,University of Extremadura | And 3 more authors.
CYTA - Journal of Food | Year: 2013

This study aimed at further investigating the antioxidant effect of smoked paprika (Pimento n La Vera, Spain) in healthy subjects. Crossover study included male students (age 20.6 + 1.7 years) who consumed smoked or non-smoked paprika (2 g/day, 20 times, over 30 days). After ingestion of smoked paprika, plasma malondialdehyde (MDA) levels were significantly (p 5 0.01) reduced versus basal and non-smoked paprika ingestion values. Reduced glutathione (GSH) levels were increased and oxidised glutathione (GSSG) levels reduced significantly (p 5 0.05) in paired subjects after consumption of smoked paprika. Smoked paprikasignificantly increased mRNA levels of glutathione peroxidase and cytochrome c oxydase versus non-smoked paprika ingestion. The low dose of polycyclic aromatic hydrocarbons (PAHs) contained in the smoked paprika could cause an activation of antioxidant defences as increasing activity of the GSH cycle and activating some antioxidant enzyme such as glutathione peroxidase and cytochrome c oxidase.


Rodriguez-Molina M.C.,Research Center Agraria | Morales-Rodriguez M.C.,Research Center Agraria | Palo Osorio C.,Research Center Agraria | Palo Nunez E.,Research Center Agraria | And 3 more authors.
Spanish Journal of Agricultural Research | Year: 2010

Root and crown rot (Tristeza disease) is an increasing problem for red pepper crop in La Vera region (Cáceres, western Spain). Field surveys were carried on in 2006 and 2007 to identify the causal agents of this disease. A Phytophthora species was isolated from diseased plants in most of the surveyed fields (27 of 36 in 2006 and 15 of 16 in 2007), while Verticillium spp. were not detected. Fifteen Phytophthora isolates were examined and identified as P. nicotianae, all of them were heterothalic isolates of mating type A2. Pathogenicity tests conducted on 'Jaranda'red pepper plants developed symptoms of wilt and root and crown rot, although disease severity differed significantly (P < 0.001) among isolates. Results indicate that P. nicotianae is the principal causal agent of the Tristeza disease of red pepper plants in La Vera region and this has several implications for the development of future disease management strategies. The host range of isolates from red pepper plants should be studied in order to establish suitable crop rotation in this region.


Guerra M.E.,Research Center Agraria | Lopez-Corrales M.,Research Center Agraria | Rodrigo J.,CSIC - Centro de Investigación y Tecnología Agroalimentaria | Wunsch A.,CSIC - Centro de Investigación y Tecnología Agroalimentaria
Acta Horticulturae | Year: 2012

In this work a PCR S-genotyping method using capillary electrophoresis detection was assayed in Japanese plum. Sweet cherry primers designed for S-RNase and SFB intron length polymorphism detection by capillary electrophoresis were evaluated in Japanese plum cultivars. Amplification of both genes was successful and amplified sizes were correlated with Japanese plum S-alleles. The S-RNase genotype of 58 Japanese plum type cultivars previously determined by other methods was confirmed using this technology and the SFB alleles of these cultivars were also determined. Allele sizes of both genes are reported for 13 different S-alleles found in Japanese plum and will allow efficient S-genotype characterization in this species.


Guerra M.E.,Research Center Agraria | Lopez-Corrales M.,Research Center Agraria | Wunsch A.,CSIC - Centro de Investigación y Tecnología Agroalimentaria | Rodrigo J.,CSIC - Centro de Investigación y Tecnología Agroalimentaria
Acta Horticulturae | Year: 2012

The reproductive process has been evaluated to ascertain the causes of lowfruit set in two Japanese plum cultivars, 'Sweet August' and 'Rubirosa'. In order to evaluate pollen germination, pollen-pistil incompatibility and ovule viability, different experiments were performed in orchard conditions. Pollen viability was determined by assessing the percentage of in vitro pollen germination. The self-(in)compatibility of both cultivars and their cross-compatibility with 'Champion' were examined by selfand cross-pollination experiments followed by observation of pollen tube growth under the microscope. Likewise, the S-RNase alleles of each cultivar were determined by PCR amplification of the S-RNase gene. Finally, ovule development was examined under the microscope. While 'Sweet August' behaved as self-incompatible, 'Rubirosa' appears to be self-compatible. The observation of pollen germination and pollen tube growth in the different crosses analysed showed that fruit set was not limited by the absence of compatible pollen. However, a high percentage of degenerated ovules were observed in flowers from both cultivars. This premature ovule degeneration is likely to be the cause of low fruit set in both cultivars.

Loading Research Center Agraria collaborators
Loading Research Center Agraria collaborators