Research Center 27 of the Ministry of Defense of the Russian Federation

Moscow, Russia

Research Center 27 of the Ministry of Defense of the Russian Federation

Moscow, Russia
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Braun A.V.,Kostroma State University | Rybal'chenko I.V.,Kostroma State University | Ponsov M.A.,Research Center 27 of the Ministry of Defense of the Russian Federation | Stavitskaya Y.V.,Research Center 27 of the Ministry of Defense of the Russian Federation | And 2 more authors.
Journal of Analytical Chemistry | Year: 2017

A method for the determination of a mustard gas biomarker (an S-hydroxyethylthioethyl adduct with albumin) in blood plasma was optimized with the use of HPLC with high-resolution tandem mass-spectrometric detection. This method is based on the hydrolysis of this adduct by the proteinase K enzyme with the formation of the following stable tripeptide with cysteine, proline, and phenylalanine: S-[HETE]-Cys- Pro-Phe. The conditions of the sample preparation of human plasma artificially contaminated by mustard gas (the selection of an aliquot portion volume and an enzyme for the hydrolysis), the mass-spectrometric detection (the selection of optimum pairs of ion reactions and high-resolution detection modes), and the gradient elution program in the HPLC separation of an analyzed mixture were optimized. The detection limit of mustard gas in human blood plasma was 1 ng/mL. The approach proposed was tested in the analysis of human blood plasma samples by the standard addition technique and also within the framework of the first official biomedical test carried out by the Organization for the Prohibition of Chemical Weapons (OPCW) in 2016, and it exhibited a good accuracy, reproducibility, and specificity of determination. © 2017, Pleiades Publishing, Ltd.


Gainullina E.T.,Research Center 27 of the Ministry of Defense of the Russian Federation | Gulikova D.K.,Research Center 27 of the Ministry of Defense of the Russian Federation | Korneev D.O.,Research Center 27 of the Ministry of Defense of the Russian Federation | Oreshkin D.V.,Research Center 27 of the Ministry of Defense of the Russian Federation | And 2 more authors.
Journal of Analytical Chemistry | Year: 2015

Current advances in the development of tools of environmental monitoring for anticholinesterase agents are considered. The aim of this analysis was to substantiate the most promising trends in the development of an up-to-date biosensor based on cholinesterase inhibition with an optimum sensitivity-analytical-signal formation time relation for the monitoring of atmospheric air for organophosphorus warfare agents and insecticides. © 2015, Pleiades Publishing, Ltd.

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