Bailey D.,Incisive Media |
Carpenter E.P.,Research Building |
Coker A.,Center for Amyloidosis and Acute Phase Proteins |
Coker S.,Center for Amyloidosis and Acute Phase Proteins |
And 13 more authors.
Acta Crystallographica Section D: Biological Crystallography | Year: 2012
The analysis reported here describes detailed structural studies of endothiapepsin (the aspartic proteinase from Endothia parasitica), with and without bound inhibitors, and human pepsin 3b. Comparison of multiple crystal structures of members of the aspartic proteinase family has revealed small but significant differences in domain orientation in different crystal forms. In this paper, it is shown that these differences in domain orientation do not necessarily correlate with the presence or absence of bound inhibitors, but appear to stem at least partly from crystal contacts mediated by sulfate ions. However, since the same inherent flexibility of the structure is observed for other enzymes in this family such as human pepsin, the native structure of which is also reported here, the observed domain movements may well have implications for the mechanism of catalysis. © 2012 International Union of Crystallography Printed in Singapore - all rights reserved.
Hsu K.,Research Building |
Lin Y.-C.,Mackay Memorial Hospital |
Chang Y.-C.,Mackay Memorial Hospital |
Chan Y.-S.,Mackay Memorial Hospital |
And 3 more authors.
Transfusion | Year: 2013
BACKGROUND: GP.Mur (Mi.III) is a glycophorin B-A-B hybrid sialoglycoprotein expressing several potent immunogens, including Mia, Mur, and Hil. GP.Mur is considered one of the most important red blood cell (RBC) phenotypes in blood banking in Southeast Asia. However, there are no antibodies commercially available for the screening of GP.Mur RBCs. STUDY DESIGN AND METHODS: To develop a direct blood polymerase chain reaction (PCR) approach for the screening of GP.Mur cells, we first confirmed the genomic sequence differences among four GP.Mur and three Mi(a-) samples by sequencing their GYP.Mur and GYPB genes. With these data, we designed PCR primers that best discriminate GYPB and GYP.Mur. Our primer design also allows the detection of other Hil+ glycophorin variants. We also constructed two plasmids - pGBi2i3 and pMiIIIi2i3 - which serve as the negative and positive control DNA, respectively, for the PCR procedure. Additionally, we designed a control PCR to be run side by side with the typing PCR. RESULTS: Because of the high specificity of our primers, we found it unnecessary to extract DNA from blood samples for PCR. We have tested this PCR method on 379 fresh and frozen blood samples. The results were further validated by serology and DNA sequencing and were shown to be completely accurate in our hand. We also found that the rapid genotyping method - high-resolution melting - can be a timesaving alternative for DNA sequencing. CONCLUSION: This direct blood PCR approach for determination of GP.Mur and related Hil+ phenotypes is reliable and economical and is expected to be useful for blood banking in Southeast Asia. © 2012 American Association of Blood Banks.
Tong N.,McMaster University |
Shmatukha A.,General Electric |
Shmatukha A.,Research Building |
Asmah P.,General Electric |
Stainsby J.,General Electric
Physics in Medicine and Biology | Year: 2010
Various aspects of RF-induced heating of guide wires during their MRI guidance have been investigated in the past. However, the previous works focused on inducing tip heating in either fully immersed or tip-immersed (and otherwise free) wires of impractical lengths in small phantoms. This study simulates real clinical conditions using a product guide wire and a same-length conductive wire partially inserted into a torso-size phantom filled with saline solution. The purpose was to identify potential safety concerns relevant to real clinical applications, as opposed to identifying the worst-case heating scenario. Significant heating occurred at the insertion point, independent of tip heating, with a strong correlation with excitation frequency-dependent imaging parameters. Heat transfer through the wire was also demonstrated to be a safety concern. From these experiments, we have been able to demonstrate additional impacting factors that increase the complexity of safety considerations for the use of conductive guide wires during MR imaging. Safety under a particular set of conditions does not imply safety in all possible conditions that can be encountered during real MRI-guided interventions. © 2010 Institute of Physics and Engineering in Medicine.
The International Institute for Sustainable Laboratories (I2SL) is pleased to acknowledge the winners of the 2015 Go Beyond Awards. Go Beyond Award winners demonstrate their commitment to excellence in sustainability in lab and other high-tech facility projects by going beyond the facility itself to consider shared resources, infrastructure, services and neighboring communities; and contribute to increased use of energy-efficient and environmentally sustainable designs, systems and products. The 2015 Go Beyond Awards were presented during a special luncheon ceremony at the 2015 I2SL Annual Conference on Monday, September 21, 2015, in San Diego, Calif. I2SL presented four 2015 Go Beyond Awards in two categories: Individual and Project. James Dykes, Sustainable Labs Canada James Dykes, a recently retired architect from Public Works and Government Services Canada (PWGSC), received an Individual Go Beyond Award for his many years of commitment to making sustainability a key factor in lab design. Dykes is the Founding President of, and driving force behind, Sustainable Labs Canada (SLCan), a non-profit organization that promotes sustainable design and operation practices in labs and other high-tech facilities. Dykes developed and strengthened relationships between SLCan and the Real Property Institute of Canada (RPIC), I2SL and other organizations in Europe with similar goals. As a member of the RPIC Board of Directors, Dykes acted as the lab business sector representative from PWGSC, ensuring content included lab-focused issues in the RPIC Real Property National Workshop Program. Over the course of his career, Dykes has served on numerous volunteer boards, delivered conference presentations on lab design, guest lectured at several universities and colleges and was an Assistant Adjunct Professor with the Univ. of Calgary for 12 years. He worked with the Labs21 program for most of its existence, and continues to participate in I2SL’s Global Sustainable Laboratory Network. Allison Paradise, My Green Lab The second Individual Award was presented to Allison Paradise, Executive Director of My Green Lab, a non-profit organization that promotes safe, sustainable practices and equipment in labs. Paradise has been a passionate champion and advocate for sustainable lab practices since before she began the My Green Lab program several years ago. Through My Green Lab, Paradise partners with organizations to implement energy reduction, water reduction, waste management and green chemistry programs; and connects lab personnel with sustainable procurement opportunities. Working with utility providers in California, Paradise prepared the “Market Assessment of Energy Efficiency Opportunities in Laboratories,” which involved a survey of equipment use and energy efficiency that was given to almost 1,200 scientists and lab operators across the U.S. The survey identified energy-efficiency opportunities in labs that Paradise is helping to drive forward through the creation of the Center for Energy Efficient Laboratories. Jackson Laboratory for Genomic Medicine, Farmington, Conn. One of this year’s Project Awards was presented to the Jackson Laboratory for Genomic Medicine, a global leader in considering the environmental impact of its facilities and operations. The Jackson Laboratory combines inviting collegial space with efficient labs, while using a variety of energy conservation measures to maximize building performance. The lab maximizes daylight while limiting peak solar loads, and utilizes high-efficiency equipment and an improved thermal envelope. To ensure indoor air quality, the lab also has a monitoring system and occupancy sensors with the ability to reduce outdoor air during unoccupied times. The building water use is more than 30% better than code compliance. Water-saving measures include the installation of water-efficient fixtures, rainwater harvesting, bioswales and native plantings. Through these measures, the lab has also reduced the amount of stormwater runoff at the property. In addition to the ongoing energy and water savings, more than 97% of construction waste was diverted from landfill throughout the project. National Univ. of Ireland, Galway Biosciences Research Building, Galway, Ireland The second Project Award was presented to the National Univ. of Ireland, Galway, Biosciences Research Building (BRB), a research lab for regenerative medicine, chem-bio and cancer. The BRB represents a “minimum energy” approach. Through careful planning and high-/low-energy zoning, the BRB integrates traditional building techniques with innovative energy-conservation solutions, resulting in an energy savings of about 70% annually against a baseline of comparable projects. The high-/low-energy zoning strategy wraps the perimeter of the building with the lowest energy use spaces, allowing for maximum daylighting and natural ventilation, while the high-energy use spaces are zoned within the “thermal sweater” of the lower use spaces, using a double wall system to separate ventilation systems and optimizing building-wide energy use. The 2015 Go Beyond Award winners can also be found on I2SL’s Website at www.i2sl.org/conference/2015/awards.html. I2SL plans to hold the Go Beyond Awards again in 2016. A call for nominations will be sent in summer 2016 and awards will be presented at the 2016 I2SL Annual Conference, taking place September 25 through 27 in Kansas City, Mis. Learn more about the I2SL Annual Conference by visiting I2SL’s Website www.i2sl.org.
Balikcioglu P.G.,Louisiana State University Health Sciences Center |
Balikcioglu M.,SAS Institute |
Gomez R.,Louisiana State University Health Sciences Center |
Vargas A.,Louisiana State University Health Sciences Center |
Chalew S.A.,Research Building
Journal of Pediatric Endocrinology and Metabolism | Year: 2013
Body mass and anti-pancreatic antibody status potentially influences the presentation of diabetes in children. We hypothesized that anti-pancreatic auto-antibody positive patients with new onset diabetes would have lower levels of insulin and C-peptide at presentation, and hence higher HbA1c. Records of children with new onset diabetes self-identified as African American were retrospectively analyzed. Patients were under 19 years of age. Anti-GAD65 antibody titer, HbA1c, blood glucose, insulin and C-peptide levels were drawn at the time of diagnosis. Patients were classified as antibody positive if anti-GAD65 was =0.5. HbA1c, insulin and C-peptide levels were considered as dependent variables in statistical models that included auto-antibody status, gender, age, body mass index z score (BMI-z score), and blood glucose as independent covariates. Records of 61 African-American children were available for analysis. There was no statistical association of auto-antibody status or initial clinical diagnosis with HbA1c, insulin or C-peptide level. BMI-z score was strongly associated with insulin (p =0.0006) and C-peptide (p < 0.0001) levels. In general, higher BMI-z score, female gender and older age were associated with higher C-peptide levels. Although potentially helpful in eventually determining etiology, pancreatic auto-antibody levels do not have an association with HbA1c, insulin or C-peptide levels in African-American children with new onset diabetes. BMI-z score had the most robust association with insulin and C-peptide levels at presentation.