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Velikonja A.,SMARTEH Research and Development of Electronic Controlling and Regulating Systems | Kramar P.,University of Ljubljana | Miklavcic D.,University of Ljubljana | Lebar A.M.,University of Ljubljana
IFMBE Proceedings | Year: 2015

This paper presents the study of electrical properties of hydrogenated planar lipid bilayers formed from thermophilic archaeal lipids extracted from Aeropyrum pernix K1. Study is focused on measuring electrical capacitance as parameter of the planar lipid bilayer thickness and breakdown voltage as parameter of planar lipid bilayer stability in electric field. The results show that both measured parameters of hydrogenated planar lipid bilayers formed from archeal lipids extracted from A. pernix K1 are not temperature dependant on measured temperature interval between 19 °C and 56 °C. Electrical capacitance is lower than in Molecular Dynamic simulations and in experiments using 2-Oleoyl-1-palmitoylsn- glycero-3-phosphocholine (POPC) lipids or 2-Oleoyl-1- palmitoyl-sn-glycero-3-phosphoserine (POPS) lipids. Breakdown voltage is higher than in experiments with POPC or POPS lipids. © Springer International Publishing Switzerland 2015.


Velikonja A.,SMARTEH Research and Development of Electronic Controlling and Regulating Systems | Santhosh P.B.,University of Ljubljana | Gongadze E.,University of Ljubljana | Kulkarni M.,University of Ljubljana | And 5 more authors.
International Journal of Molecular Sciences | Year: 2013

In this work, a theoretical model describing the interaction between a positively or negatively charged nanoparticle and neutral zwitterionic lipid bilayers is presented. It is shown that in the close vicinity of the positively charged nanoparticle, the zwitterionic lipid headgroups are less extended in the direction perpendicular to the membrane surface, while in the vicinity of the negatively charged nanoparticle, the headgroups are more extended. This result coincides with the calculated increase in the osmotic pressure between the zwitterionic lipid surface and positively charged nanoparticle and the decrease of osmotic pressure between the zwitterionic lipid surface and the negatively charged nanoparticle. Our theoretical predictions agree well with the experimentally determined fluidity of a lipid bilayer membrane in contact with positively or negatively charged nanoparticles. The prospective significance of the present work is mainly to contribute to better understanding of the interactions of charged nanoparticles with a zwitterionic lipid bilayer, which may be important in the efficient design of the lipid/nanoparticle nanostructures (like liposomes with encapsulated nanoparticles), which have diverse biomedical applications, including targeted therapy (drug delivery) and imaging of cancer cells. © 2013 by the authors; licensee MDPI, Basel, Switzerland.

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