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Sicuia O.-A.,Research and Development Institute for Plant Protection | Oancea F.,Romanian National Institute for Research and Development in Chemistry and Petrochemistry | Constantinescu F.,Research and Development Institute for Plant Protection | Dinu S.,Research and Development Institute for Plant Protection | Cornea C.P.,University of Bucharest
Romanian Biotechnological Letters | Year: 2012

Mulching represents a profitable technological step in crop production. Beside benefits, vegetal mulching technologies trigger also some phytosanitary risks. This disadvantage can be prevented trough bio-activation of the vegetal mulch by adding antagonistic beneficial bacteria. Three Bacillus strains were selected and used as bio-active agents in vegetal mulch, based on their antifungal properties and biofertilizer activity. The selected strains were analyzed for traits related with plant growth promotion and biocontrol of some fungal diseases. The results proved that the strains are competitive soil bacteria which can be successfully used for bio-activate the vegetal mulch.

Alina S.O.,University of Bucharest | Alina S.O.,Research and Development Institute for Plant Protection | Constantinscu F.,Research and Development Institute for Plant Protection | Petruta C.C.,University of Bucharest
Romanian Biotechnological Letters | Year: 2015

Biological control of plant pathogens and plant growth promotion by biological means is the late tendency in biotechnological approaches for agricultural improvement. Such an approach is considering the environmental protection issues without neglecting crop needs. In the present study, we are reviewing Bacillus spp. biocontrol and plant growth promoting activity. As Bacillus genus is a large bacterial taxon, with grate physiological biodiversity, we are describing some inter-grouping, differences and similarities between Bacillus species, especially related to Bacillus subtilis. © 2015 University of Bucharest.

Cornea C.P.,University of Bucharest | Roming F.I.,University of Bucharest | Sicuia O.A.,Research and Development Institute for Plant Protection | Voaides C.,University of Bucharest | And 2 more authors.
Romanian Biotechnological Letters | Year: 2016

Biosurfactants are surface-active molecules produced by different microorganisms, including Lactobacillus spp. strains, with emulsifying properties. For their emulsifying activities as well as for the inhibition of some pathogenic microorganisms, biosurfactants produced by lactobacilli could find important applications in food industry. The aim of this study was to screen for biosurfactant production in several lactic acid bacteria (LAB) strains. Ten LAB strains selected previously for antifungal activities were used in experiments. The biosurfactant production was evaluated both in supernatant and in cells cultivated in Man-Sharp-Rogosa (MRS) broth. Oil spreading test, emulsification activity, and drop collapse methods were used for evaluation the biosurfactant production. Antimicrobial action of biosurfactants against Escherichia coli, Bacillus cereus, Staphylococcus aureus, Candida parapsilopsis and mycotoxigenic fungi was also examined. Molecular analyses were performed for characterization of selected bacteria. Three out of ten tested LAB strains exhibited clear biosurfactant production. The emulsifying action of kerosene was detected both in extracts from supernatants and from cells. For the strains L26 and L35, the level of cell-bound biosurfactant production was higher than the excreted one, for L61 the emulsifying activity was higher in supernatant, mainly against edible oils. The antimicrobial action of extracted biosurfactants suggests that these compounds could be involved in the inhibitory action of LAB. This study demonstrates that two strains of L.plantarum (L26 and L35) and one strain of L.brevis (L61) were able to produce cell-bound and excreted biosurfactants. High emulsifying activity was detected on edible oils, giving stable emulsions, suggesting the possible use of these biosurfactants in food industry. © 2016 University of Bucharest.

Peteu S.F.,Research and Development Institute for Plant Protection | Peteu S.F.,University of Michigan
Proceedings of the International Semiconductor Conference, CAS | Year: 2010

A fresh perspective of bio-inspired engineering is applied to emerging concepts, designs, applications in the area of responsive delivery of countermeasures. In particular, cutting edge, broad impact applications are examined, such as nanorobotics or integrated sensing-actuating functions down to nano-scale. These can be used towards the recognitive release of therapeutic agents. The ideas and concepts expressed are fertilized with a biomimetic twist, thereby leveraging nature's million years of sustainable evolution. Moreover, a radically new look is emphasized: of the hierarchically multi-level integrated self-healed biosystems that shall emulate positive revolutionary changes for the future bio-inspired, smart therapies with personalized, design. © 2010 IEEE.

Ploaie P.G.,Research and Development Institute for Plant Protection | Chireceanu C.,Research and Development Institute for Plant Protection
Romanian Biotechnological Letters | Year: 2012

A study was performed on Grape yellows from 2008-2010, in five commercial grapevine growing regions in Romania. The presence of typical yellowing symptoms resembling Flavescence dorée and Bois noir diseases, described in West Europe, and on other continents, were detected on Vitis vinifera cvs. Chardonnay, Hamburg, Merlot, Feteascǎ regalǎ, Cabernet Sauvignon, and Pinot Noir. Natural foci with Convolvulus arvensis, with symptoms of stolbur, Cichorium inthybus and Taraxacum officinale with symptoms of yellowing and virescence were detected in South of Romania and Trifolium repens with symptoms of clover phyllody and clover dwarf (aster yellows) were detected in Transylvania. Insect vectors as Hyalesthes obsoletus, Reptalus panzeri, Dictyophara europaea, Scaphoideus titanus, Macrosteles laevis and M. viridigriseus were detected also in natural foci. The vector H. obsoletus collected from grapevine cultivars transmitted stolbur MLO to periwinkle plants. By electron microscopy, on ultrathin sections, structures resembling cell wall deficient bacteria (MLOs, phytoplasma) were detected in the sieve elements of grapevine, and in the sediment of purified material. Using a polyclonal antiserum raised in rabbits with Aster yellows antigen, growth in artificial media, an indirect dot blot immunoassay (ELISA) technique was used and etiological agent as stolbur and aster yellows were identified. These results are first experimental proofs regarding the association of stolbur and aster yellows with grape yellows in Romania. © 2012 University of Bucharest.

Peteu S.F.,Research and Development Institute for Plant Protection | Oancea F.,Research and Development Institute for Plant Protection | Sicuia O.A.,Research and Development Institute for Plant Protection | Constantinescu F.,Research and Development Institute for Plant Protection | Dinu S.,Research and Development Institute for Plant Protection
Polymers | Year: 2010

This review outlines the responsive polymer methods currently in use with their potential application to plant protection and puts forward plant-specific mechanisms as stimuli in newly devised methods for smart release of crop protection agents (CPAs). CPAs include chemicals (fungicides, insecticides, herbicides), biochemicals (antibiotics, RNA-based vaccines for plant viruses), semiochemicals (pheromones, repellents, allomones), microbial pesticides, growth regulators (insect and plant) or micronutrients, all with crop protection effects. This appraisal focuses on emerging uses of polymer nano-encapsulated CPAs. Firstly, the most interesting advances in controlled release methods are critically discussed with their advantages and drawbacks. Secondly, several plant-specific stimuli-based smart methods are anticipated for use alongside the polymer nano- or micro-capsules. These new CPA release methods are designed to (i) protect plants against infection produced by fungi or bacteria, and (ii) apply micro-nutrients when the plants need it the most. Thus, we foresee (i) the responsive release of nano- encapsulated bioinsecticides regulated by plant stress enzymes, and (ii) the delivery of micro-nutrients synchronized by the nature or intensity of plant root exudates. Such continued advances of nano-scale smart polymer-based CPAs for the protection of crops herald a "small revolution" for the benefit of sustainable agriculture. © 2010 by the authors.

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