Time filter

Source Type

Zheng Z.-Z.,Research and Development Center for Medicine Plant and Plant Drugs | Ming Y.-L.,Research and Development Center for Medicine Plant and Plant Drugs | Ming Y.-L.,Xiamen University | Chen L.-H.,Research and Development Center for Medicine Plant and Plant Drugs | And 4 more authors.
Oncology Reports | Year: 2014

An intestinal bacterial metabolite of ginseng protopanaxadiol saponin, 20-O-(β-D-glucopyranosyl)-20(S)-protopanaxadiol (compound K), has been reported to induce apoptosis in a variety of cancer cells. However, the precise mechanisms induced by compound K in human hepatocellular carcinoma (HCC) cells remain unclear. In order to examine possible apoptotic mechanisms, we investigated the anticancer effect of compound K in MHCC97-H. MTT assay showed that compound K inhibited the proliferation of MHCC97-H cells with a relatively low toxicity in normal hepatoma cells. Cell cycle progression and cell staining showed an increase in apoptotic sub-G1 fraction. Treatment of MHCC97-H with compound K also induced a reduction in mitochondrial membrane potential (Δψm) and DNA damage. Further study showed that compound K upregulated Fas, FasL, Bax/Bcl-2 ratio and downregulated pro-caspase-9, pro-caspase-3 in a dose-dependent manner, and it also inhibited Akt phosphorylation. These results suggest that compound K significantly inhibits cell proliferation and induces apoptosis in MHCC97-H cells through Fas- and mitochondria-mediated caspase-dependent pathways in human HCC cells.


Ming Y.-L.,Research and Development Center for Medicine Plant and Plant Drugs | Zheng Z.-Z.,Research and Development Center for Medicine Plant and Plant Drugs | Chen L.-H.,Research and Development Center for Medicine Plant and Plant Drugs | Tong Q.-X.,Research and Development Center for Medicine Plant and Plant Drugs
Chinese Traditional and Herbal Drugs | Year: 2010

Objective: In order to investigate the sensitization of 20-O-β-D-glucopyranoside-20(S)-protopanaxadiol (ginsenoside IH901) with several common drugs CTX, 5-FU, cDDP in hepatocellular carcinoma (HCC) Bel-7402 and SMMC-7721. Methods: The inhibitory rates on HCC in IH901, CTX, 5-FU, cDDP, IH901 with CTX, IH901 with 5-FU, and IH901 with cDDP groups were detected by MTT assay. The synergism index, Q value, was calculated to judge the sensitization of combined drugs. Results: The IH901 with CTX group could increase the inhibitory effect on cell proliferation of HCC significantly with the synergistic effect. The inhibitory rate in IH901 with 5-FU group was just superposition on two cell lines. IH901 with cDDP group was still superposition on Bel-7402, but lightly had the synergistic effect on SMMC-7721. Conclusion: The IH901 could increase the inhibitory effect of CTX on cell proliferation of HCC and the sensitizasion on chemotherapy and also have wide adaptability.

Loading Research and Development Center for Medicine Plant and Plant Drugs collaborators
Loading Research and Development Center for Medicine Plant and Plant Drugs collaborators