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Strata F.,University of California at San Francisco | Strata F.,University of Parma | Giritharan G.,University of California at San Francisco | Piane L.D.,Reproductive Medicine and IVF Unit | And 3 more authors.
Reproductive Sciences | Year: 2015

Preimplantation culture of mouse embryos has been suggested to result in reduced anxiety-like behavior in adulthood. Here, we investigated the effects of in vitro fertilization (IVF), embryo culture, and different diets on anxiety-like behavior using the elevated plus maze (EPM). We hypothesized that exposure to suboptimal conditions during the preimplantation stage would interact with the suboptimal diet to alter behavior. The expression of genes related to anxiety was then assessed by quantitative real-time polymerase chain reaction in various brain regions. When fed a normal diet during gestation and a moderately high-fat Western diet (WD) postnatally, naturally conceived (NC) and IVF mice showed similar anxiety-like behavior on the EPM. However, when fed a low-protein diet prenatally and a high-fat diet postnatally (LP/HF), NC mice showed a modest increase in anxiety-like behavior, whereas IVF mice showed the opposite: a strongly reduced anxiety-like behavior on the EPM. The robust reduction in anxiety-like behavior in IVF males fed the LP/HF diets was, intriguingly, associated with reduced expression of MAO-A, CRFR2, and GABA markers in the hypothalamus and cortex. These findings are discussed in relation to the developmental origin of health and disease hypothesis and the 2-hit model, which suggests that 2 events, occurring at different times in development, can act synergistically with long-term consequences observed during adulthood. © The Author(s) 2014. Source

Kara M.,Bozok University | Kutlu T.,Reproductive Medicine and IVF Unit | Sofuoglu K.,Reproductive Medicine and IVF Unit | Devranoglu B.,Reproductive Medicine and IVF Unit | Cetinkaya T.,Reproductive Medicine and IVF Unit
Iranian Journal of Reproductive Medicine | Year: 2012

Background: Estradiol (E 2) is required for follicular development and play an important role in embryo implantation. Objective: The aim of this study was to assess the impact of serum E 2 levels on the day of hCG administration in IVF-ICSI patients who are performed controlled ovarian hyperstimulation (COH). Materials and Methods: A total of 203 women who were undergone one time IVF cyclus were evaluated in this cross sectional study. All the patients were treated either with long protocol or with microdose flare protocol. The patients were categorized into five groups according to the serum E 2 levels on the day of hCG administration. Results: The mean number of the retrieved oocytes was (NRO) 10.6±6.7, mean fertilization rate was 55.7±24.8, and implantation rate was 9.0±19.2. Of 203 patients, 43 (21%) patients were pregnant. When the overall results are examined, the number of the retrieved oocytes and the number of transferred embryos were better in patients with serum E 2 levels >4000 pg/ml and these values were statistically significant. There were no statistical difference in patients 37 years or older. In women ≤36 years old, the IVF-ICSI outcomes were better in patients with serum E 2 levels >4000 pg/ml. Conclusion: In spite of the lack of high quality evidence to support a positive association between serum E 2 levels and IVF-ICSI outcomes, this study shows that high E 2 levels during COH might be associated with an increased potential of pregnancy depending on better ovarian response. When the overall results are examined, the best scores were in patients with serum E 2 levels >4000 pg/ml. Source

Almagor M.,Reproductive Medicine and IVF Unit | Almagor M.,Hebrew University of Jerusalem | Or Y.,Reproductive Medicine and IVF Unit | Or Y.,Hebrew University of Jerusalem | And 4 more authors.
Journal of Assisted Reproduction and Genetics | Year: 2015

Purpose: This is a retrospective analysis of the morphokinetics, prevalence, and implantation potential of embryos with irregular first and second cleavages as identified by time-lapse microscopy. Methods: The study included 253 women who underwent 387 assisted reproduction treatments with intracytoplasmic sperm injection (ICSI). Each patient was assigned to one of three groups based on embryo cleavage results. In group I, one to two embryos per cycle showed irregular cleavage; group II, at least three embryos with abnormal cleavage; and in group III (the control group), all embryos cleaved normally. The number of embryos that cleaved from 1 to ≥3 cells or from 2 to ≥5 cells for each patient was recorded. Their prevalence and association with women’s characteristics and pregnancy outcome were evaluated. Results: The prevalence of irregular cleavage was 15.6 % among 1772 ICSI embryos. In 101 cycles, 1–2 embryos per cycle showed irregular cleavage (group I). In 32 cycles, at least 3 embryos showed abnormal cleavage (group II). In 254 cycles, all embryos cleaved normally (group III). The average age of the women in group II was significantly lower in comparison with groups I and III (32.5 ± 4.2 vs. 35.1 ± 4.9 and 35.5 ± 5.1, respectively, p < 0.02). In comparison of groups I and II, the odds ratio for ≥3 embryos with irregular cleavage in women younger than 35 was 3.48 (95 % CI, 1.28 to 9.46). Embryos with irregular cleavage were transferred in 16 women. Three live births were achieved following the transfer of single blastocysts derived from embryos with irregular cleavage from two to five cells. Conclusions: Early embryos with irregular cleavage are significantly more prevalent in younger women. When these embryos develop to the blastocyst stage, they may have normal implantation potential, leading to the birth of healthy babies. © 2015, Springer Science+Business Media New York. Source

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