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Qin J.,Maternal and Child Health Hospital of Hunan Province | Qin J.,Central South University | Liu X.,Central South University | Sheng X.,Maternal and Child Health Hospital of Hunan Province | And 2 more authors.
Fertility and Sterility | Year: 2016

Objective To determine whether there are any increases in pregnancy-related complications and adverse pregnancy outcomes in singleton pregnancies after assisted reproductive technology (ART) compared with those conceived naturally. Design Meta-analysis. Setting University-affiliated teaching hospital. Patient(s) Singleton pregnancies conceived with ART and naturally. Intervention(s) PubMed, Google Scholar, Cochrane Libraries and Chinese database were searched through March 2015 to identify studies that met pre-stated inclusion criteria. Either a fixed- or a random-effects model was used to calculate the overall combined risk estimates. Subgroup analysis was performed to explore potential heterogeneity moderators. Main Outcome Measure(s) Pregnancy-related complications and adverse pregnancy outcomes. Result(s) Fifty cohort studies comprising 161,370 ART and 2,280,241 spontaneously conceived singleton pregnancies were identified. The ART singleton pregnancies had a significantly increased risk of pregnancy-induced hypertension (relative risk [RR] 1.30, 95% confidence interval [CI] 1.04-1.62; I2 = 79%), gestational diabetes mellitus (RR 1.31, 95% CI 1.13-1.53; I2 = 6%), placenta previa (RR 3.71, 95% CI 2.67-5.16; I2 = 72%), placental abruption (RR 1.83, 95% CI 1.49-2.24; I2 = 22%), antepartum hemorrhage (RR 2.11, 95% CI 1.86-2.38; I2 = 47%), postpartum hemorrhage (RR 1.29, 95% CI 1.06-1.57; I2 = 65%), polyhydramnios (RR 1.74, 95% CI 1.24-2.45; I2 = 0%), oligohydramnios (RR 2.14, 95% CI 1.53-3.01; I2 = 0%), cesarean sections (RR 1.58, 95% CI 1.48-1.70; I2 = 92%), preterm birth (RR 1.71, 95% CI 1.59-1.83; I2=80%), very preterm birth (RR 2.12, 95% CI 1.73-2.59; I2 = 90%), low birth weight (RR 1.61, 95% CI 1.49-1.75; I2 = 80%), very low birth weight (RR 2.12, 95% CI 1.84-2.43; I2 = 67%), small for gestational age (RR 1.35, 95% CI 1.20-1.52; I2 = 82%), perinatal mortality (RR 1.64, 95% CI 1.41-1.90; I2=45%), and congenital malformation (RR 1.37, 95% CI 1.29-1.45; I2=41%). Relevant heterogeneity moderators have been identified by subgroup analysis. Sensitivity analysis yielded consistent results. No evidence of publication bias was observed. Conclusion(s) The ART singleton pregnancies are associated with higher risks of adverse obstetric outcomes. Obstetricians should manage these pregnancies as high risk. © 2016 American Society for Reproductive Medicine. Source

Liu C.J.,Reproductive Center
Zhonghua nan ke xue = National journal of andrology | Year: 2011

To explore the relationship of sperm DNA damage with unexplained recurrent spontaneous abortion (URSA). Sperm DNA fragmentation was evaluated by sperm chromatin dispersion (SCD), and the results were expressed in terms of DNA fragmentation index (DFI). DFIs were measured in the male partners of 56 women with URSA (the experiment group) and of 31 without URSA (the control group). The DFI was shown to be (11.0% - 56.9%) in the experiment group, 21 (37.5%) of the subjects over 30%, as compared with (10.0% -36.8%) in the control group, only 8 (25.8%) of the subjects over 30%, significantly higher in the former than in the latter (29.4% vs 25.5%, P < 0.05). There is a relationship between sperm DNA damage and URSA. Source

Simerman A.A.,University of California at Los Angeles | Hill D.L.,Reproductive Center | Grogan T.R.,University of California at Los Angeles | Elashoff D.,University of California at Los Angeles | And 5 more authors.
Fertility and Sterility | Year: 2015

Objective: To determine whether follicular fluid (FF) cortisol levels affect cumulus cell (CC) lipid content during oocyte meiotic resumption, and whether CCs express genes for glucocorticoid action. Design: Prospective cohort study. Setting: Academic medical center. Patient(s): Thirty-seven nonobese women underwent ovarian stimulation for in vitro fertilization (IVF). Intervention(s): At oocyte retrieval, FF was aspirated from the first follicle (>16 mm in size) of each ovary and pooled CCs were collected. Main Outcome Measure(s): Follicular fluid cortisol and cortisone analysis was performed with the use of liquid chromatography-tandem mass spectrometry. CCs were stained with lipid fluorescent dye Bodipy FL C16 to determine lipid content with the use of confocal microscopy. Quantitative real-time polymerase chain reaction was used to detect CC gene expression of 11β-hydroxysteroid dehydrogenase (11β-HSD) types 1 and 2, glucocorticoid receptor (NR3C1), lipoprotein lipase (LPL), and hormone-sensitive lipase (HSL). Result(s): Adjusting for maternal age, FF cortisol levels negatively correlated with CC lipid content and positively correlated with numbers of total and mature oocytes. CCs expressed genes for 11β-HSD type 1 as the predominant 11β-HSD isoform, NR3C1, LPL, and HSL. Conclusion(s): FF cortisol levels may regulate CC lipolysis during oocyte meiotic resumption and affect oocyte quality during IVF. ©2015 by American Society for Reproductive Medicine. Source

Guo Q.-H.,Shandong University | Yang H.-J.,Reproductive Center | Wang S.-D.,Shandong University
European Review for Medical and Pharmacological Sciences | Year: 2015

OBJECTIVE: Olanzapine, a D2/5-HT2 antagonist, is often used as an atypical antipsychotic drug in clinical. Previous research has found its new pharmacological influence on enhancing the differentiation of neural stem cells (NSCs) to oligodendrocyte-like cells (ODLCs). Glioblastomas are associated with poor prognoses owing to the glioma stem-like cells (GSLCs), which have a great many of similarities with adult NSCs. Hence, in this article, we aim to study the effects and associated mechanisms of olanzapine on GSLCs derived from human U87MG glioblastoma cell lines. MATERIALS AND METHODS: The methyl thiazolyl tetrazolium (MTT) colorimetric assay was conducted to investigate the effects of olanzapine on cell viability of GSLCs. Flow cytometric analysis was applied to study the cell cycle dynamics of GSLCs and Cell Counting Kit-8 (CCK-8) was used to further investigate the proliferation of GSLCs after treated with olanzapine or dimethyl sulfoxide (DMSO) for 48 h. Cell differentiation assay was carried out to study the differentiation of GSLCs and then Image-Pro Plus image analysis was used to measure the protrusion length of the differentiated cells. Furthermore, the confocal [Ca2+]c measurement was conducted to observe the influence of olanzapine on the opening function of Ca2+ channel. After the application of olanzapine for 48 h, RT-PCR was conducted to measure mRNA levels of calciumsensing receptor (CaSR) and stromal interaction molecule 1(STIM1), and Western blotting analysis was carried out to examine the expression of myelin basic protein (MBP), glial fibrillary acidic protein (GFAP), CaSR protein, STIM1 protein and β-catenin protein. RESULTS: Our results demonstrated that olanzapine inhibited the proliferation of GSLCs by arresting cell cycle in G0/G1 phase and facilitated the differentiation of such cells to ODLCs. After treated with olanzapine for 48 h, cells were very sensitive to 100 mM K+ stimulation, with increased spontaneous calcium wave. We also found olanzapine increased the protein expression of MBP and GFAP. In addition, the mRNA transcription and protein expression of CaSR and STIM1 were enhanced after treated with olanzapine for 48h, while the protein expression of β-catenin was suppressed. CONCLUSIONS: Our results suggest that olanzapine modulates the Wnt signaling pathway through activating the Ca2+ pathway and restraining the β-catenin pathway, leading to the differentiation of GSLCs to ODLCs. It provides exciting prospects that olanzapine might be a new novel chemotherapeutic modality targeting GSLCs for the treatment of glioblastomas. Source

To study ovarian development in vitrification of embryos born mice and expression of growth differentiation factor 9 (GDF-9) in its. The vitrification recovery embryos (vitrified-embryo group) and fresh embryos (fresh-embryo group) were transplanted into pseudopregnant mice, respectively. The female offspring mice in two groups were sacrificed on the 3(rd), 7(th), 14(th), 21(st), 28(th) and 60(th) day after birth, the ovarian tissues were taken, 6 mice in each time point of each group. The ovarian development was observed by HE staining, the expression of GDF-9 mRNA and protein at each time point of two groups were detected by reverse transcription (RT)-PCR and western blot. HE staining showed that no abnormal ovarian development was observed in offsprings at two groups. On the 3(rd), 7(th), 14(th), 21(st), 28(th) and 60(th) day after birth, the expression of GDF-9 mRNA in vitrified-embryo group were 0.14 ± 0.07, 0.42 ± 0.16, 1.00 ± 0.24, 1.59 ± 0.28, 2.05 ± 0.32 and 2.23 ± 0.21, respectively, which in fresh-embryo group were 0.13 ± 0.06, 0.45 ± 0.18, 1.00 ± 0.21, 1.56 ± 0.26, 2.01 ± 0.37 and 2.26 ± 0.23, respectively, there was no statistical difference between two groups (P > 0.05); the expression of GDF-9 protein in vitrified-embryo group were 0.040 ± 0.030, 0.120 ± 0.060, 0.170 ± 0.030, 0.250 ± 0.040, 0.320 ± 0.060 and 0.330 ± 0.010, respectively, which in fresh-embryo group were 0.030 ± 0.020, 0.110 ± 0.040, 0.150 ± 0.010, 0.210 ± 0.020, 0.360 ± 0.070 and 0.350 ± 0.030, respectively, there was no statistical difference between two groups (P > 0.05). The ovarian morphology in vitrification of embryos born mice and expression of GDF-9 in ovary has no any obvious change. Source

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