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Khouadja S.,University of Monastir | Suffredini E.,Reparto Adempimenti Comunitari e Sanita Pubblica | Spagnoletti M.,University of Rome La Sapienza | Croci L.,Reparto Adempimenti Comunitari e Sanita Pubblica | And 2 more authors.
World Journal of Microbiology and Biotechnology | Year: 2013

The occurrence of the hemolysin genes, tdh and trh, in Vibrio parahaemolyticus strains isolated from environmental samples collected from various exported seafood products comprising of fishes and shellfish (Mytilus edulis and Crassostrea gigas) or seawater, was studied. Eight strains were confirmed as V. parahaemolyticus by toxR -based polymerase chain reaction and only one strain out of these 8 strains was positive for tdh and trh genes. Toxigenic V. parahaemolyticus isolates are present in Tunisian coastal areas and they may also be present in Tunisian exported seafood products. © 2013 Springer Science+Business Media Dordrecht. Source


Khouadja S.,University of Monastir | Suffredini E.,Reparto Adempimenti Comunitari e Sanita Pubblica | Baccouche B.,University of Monastir | Croci L.,Reparto Adempimenti Comunitari e Sanita Pubblica | Bakhrouf A.,University of Monastir
Environmental Monitoring and Assessment | Year: 2014

Pathogenic Vibrio species are an important cause of foodborne illnesses. The aim of this study was to describe the occurrence of potentially pathogenic Vibrio species in the final effluents of a wastewater treatment plant and the risk that they may pose to public health. During the 1-year monitoring, a total of 43 Vibrio strains were isolated: 23 Vibrio alginolyticus, 1 Vibrio cholerae, 4 Vibrio vulnificus, and 15 Vibrio parahaemolyticus. The PCR investigation of V. parahaemolyticus and V. cholerae virulence genes (tlh, trh, tdh, toxR, toxS, toxRS, toxT, zot, ctxAB, tcp, ace, vpi, nanH) revealed the presence of some of these genes in a significant number of strains. Intraspecies variability and genetic relationships among the environmental isolates were analyzed by random amplified polymorphic DNA-PCR (RAPD-PCR). We report the results of the first isolation and characterization of an environmental V. cholerae non-O1 non-O139 and of a toxigenic V. parahaemolyticus strain in Tunisia. We suggest that non-pathogenic Vibrio might represent a marine reservoir of virulence genes that can be transmitted between strains by horizontal transfer. © 2014, Springer International Publishing Switzerland. Source

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