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Lee S.,Korea Advanced Institute of Science and Technology | Lee K.H.,Korea Advanced Institute of Science and Technology | Ha J.-S.,Reons Innovative Medicines Institute | Lee S.-G.,Korea Research Institute of Bioscience and Biotechnology | Kim T.K.,Reons Innovative Medicines Institute
Angewandte Chemie - International Edition | Year: 2011

Seeing into cells: Genetically engineered, labeled ferritin is used to form biological nanoparticles displaying bait (B) and prey (P) molecules inside cells (see picture). Specific bait-prey interactions induce the interconnected assembly of nanoparticles into nanoclusters on a timescale of seconds. This phenotypic readout with a small molecule as an inducible switch was used to directly visualize dynamic molecular interactions within living cells. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source


Lee S.,Korea Advanced Institute of Science and Technology | Ha J.-S.,Reons Innovative Medicines Institute | Lee S.-G.,Korea Research Institute of Bioscience and Biotechnology | Kim T.K.,Reons Innovative Medicines Institute | Kim T.K.,Ulsan National Institute of Science and Technology
Journal of the American Chemical Society | Year: 2012

We present a novel phenotypic readout using inducible, biosynthetic nanoscaffolds to directly visualize dynamic molecular interactions within living cells at the single-cell level with high sensitivity and selectivity. Labeled ferritin is used to form biological nanoparticles inside cells. Specific supramolecular assembly of ferritin-derived nanoparticles induces highly clustered nanoscaffolds. These inducible biosynthetic nanoscaffolds are used as the artificial recruitment/redistribution platform for monitoring interactions of a small molecule with its target protein(s) inside living cells. © 2012 American Chemical Society. Source

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