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Fortaleza, Brazil

Neves M.L.C.,Renorbio UECE | Neves M.L.C.,Federal Rural University of Pernambuco | Porto T.S.,Federal Rural University of Pernambuco | Souza-Motta C.M.,Federal University of Pernambuco | And 5 more authors.
Fluid Phase Equilibria | Year: 2012

The partition and recovery behavior of phytase, produced by solid-state cultivation utilizing citrus pulp as substrate, was determined in an aqueous two-phase system (ATPS), composed by polyethylene glycol (PEG-citrate). The 2 4 factorial design was conducted to evaluate the effect of the following independent variables: PEG molar mass, PEG and citrate concentrations and pH, while partition coefficient, recovery and purification factor were selected as the variables responses. The best recovery in phytase activity (115.24%) condition was obtained with 26% (w/w) PEG 8000 (g/mol), 12% (w/w) sodium citrate at pH 8.0, showing a purification factor of 1.35. However, the highest partition coefficient (14.42) was observed within a 26% (w/w) PEG 400 (g/mol) and a 20% (w/w) sodium citrate at pH 6.0. The independent variables which more influenced on the partition coefficient and recovery were citrate concentration and PEG mass molar, respectively. The results suggest that PEG-citrate ATPS is an interesting and efficient alternative to partition and recovery of phytase from fermentation broth produced by Absidia blakesleeana URM5604. © 2012 Elsevier B.V. Source


Neves M.L.C.,Renorbio UECE | Neves M.L.C.,University of Pernambuco | Da Silva M.F.,Federal Rural University of Pernambuco | Souza-Motta C.M.,Federal University of Pernambuco | And 6 more authors.
Molecules | Year: 2011

Brazil is known for its great potential for production of renewable resources such as agro-industrial residues. These residues can be used as alternative sources of new products. Meanwhile, solid-state fermentation, with its advantages of energy conservation and pollution reduction, has been identified as a process of great potential for the production of bioactive compounds, especially enzymes. In the present work, a 23 factorial design was used to evaluate the effects of pH, temperature and moisture on the production of phytase and xylanase by Lichtheimia blakesleeana URM 5604 through the fermentation of citrus pulp. Statistical analyses of the results showed that the only the pH influenced the production of these enzymes, with the best phytase production (264.68 U/g) ocurring at pH 6.0, 34 °C, initial moisture 50%, after 48 hours of culture. The best conditions for xylanase production (397.82 U/g) were fermentation for 120 hours at pH 4.0, 26 °C and initial moisture of 70%. The best parameters for the simultaneous production of phytase (226.92 U/g) and xylanase (215.59 U/g) were determined to be initial moisture of 50%, pH 6.0, 26 °C, and 48 hours of fermentation. © 2011 by the authors; licensee MDPI, Basel, Switzerland. Source


Silva G.M.M.,Renorbio UECE | Bezerra R.P.,Federal Rural University of Pernambuco | Teixeira J.A.,University of Minho | Porto T.S.,Federal Rural University of Pernambuco | And 4 more authors.
Electronic Journal of Biotechnology | Year: 2015

Background: Streptomyces sp. DPUA 1576 from Amazon lichens was studied to protease and fibrinolytic production. A 22 factorial experimental design was applied to optimize its protease enzyme production using two independent variables, namely soybean flour and glucose concentrations. Results: The optimal conditions to obtain high protease production (83.42 U/mL) were 1.26% soybean flour and 1.23% glucose concentration. A polynomial model was fitted to correlate the relationship between the two variables and protease activity. In relation to fibrinolytic activity, the highest activity of 706.5 mm2 was obtained at 1.7% soybean flour and 1.0% glucose concentration, which was 33% higher than plasmin. Fibrinolytic production was not optimized in the studied conditions. Conclusions: These results show that the optimization of the culture medium can enhance protease production, thus becoming a good process for further research. In addition, Streptomyces sp. DPUA 1576, isolated from Amazon lichens, might be a potential strain for fibrinolytic protease production. © 2014 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserved. Source

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