Li Z.,Renmin Hospital of Jiaozuo |
Li Z.,Central South University |
Zhao J.,Central South University |
Li Q.,Renmin Hospital of Jiaozuo |
And 4 more authors.
Cell Stress and Chaperones | Year: 2010
K562 cells and peripheral blood mononuclear cells were treated with hydrogen peroxide (H2O2) to determine the expression of Krüppel-like factor (KLF) 4. A full-length complementary DNA or an anti-sense oligonucleotide of KLF4 was transfected into cells, and expressions of B-cell lymphoma/leukemia-2 (bcl-2) and bcl-2-associated X (bax) proteins were analyzed. The results showed that H2O2 treatment of cells resulted in an increase in KLF4 levels; KLF4 induced apoptosis and slowed cell growth, potentially resulting from up-regulation of bax and down-regulation of bcl-2. Transcriptional activities on bcl-2 and bax were promoted following KLF4 overexpression potentially through KLF4 binding sites on corresponding promoters. All results indicate that KLF4 induces apoptosis in leukemia cells involving the bcl-2/bax pathway during H2O2 stimulation, suggesting a potential mechanism for research on drug-induced apoptosis. © 2010 Cell Stress Society International.