Feng-Chun Z.,Shanghai JiaoTong University |
Feng-Chun Z.,Ruijin Hospital of Shanghai |
Fei-Fei N.,Shanghai JiaoTong University |
Hai-Yan X.,Shanghai JiaoTong University |
And 4 more authors.
Tumor | Year: 2012
Objective: To explore the enrichment of breast cancer stem cells derived from primary breast cancer tissues by using a suspended culture system, and to analyze the relationship between mammosphere formaiton and the clinicopathological features of patients with breast cancer. Methods: Breast cancer cells were obtained from 45 patients with breast cancer and cultured in a suspended culture system to generate mammospheres. The percentage of breast cancer cells with a phenotype of CD44+/CD24 low/- in mammosphere cells was assessed by flow cytometry (FCM), and the expressions of Nanog , KLF 4, OCT -4, SOX 2 and MDR 1 genes were detected by real-time fluorogenic quantitative- PCR (RFQ-PCR). The relationship between mammosphere formation and the clinicopathological features of patients with breast cancer was analyzed. Results: The mammospheres were formed on the 14th day during the period of suspended culture. FCM result demonstrated that the percentage of breast cancer cells with a phenotype of CD44+/CD24 low/- in mammosphere cells derived from primary breast cancer cells was significantly higher than that in the primary breast cancer cells (24.71% vs 1.30%,P < 0.05). The mammosphere formation was positively related to the age of breast cancer onset, age at menarche, menstrual status, histological grading, progestogen receptor status, human epidermal growth factor receptor 2 (HER-2) status and cancer recurrence (P < 0.05). However, the disease-free survival (DFS) and the overall survival (OS) were not associated with mammosphere formation (P > 0.05). Conclusion: Primary breast cancer stem cells can be enriched by using a suspended culture system, and the mammosphere formation was positively associated with some clinicopathological characteristics, so it suggests that the mammosphere formation may become a potential predictive factor in the prognosis of breast cancer. © 2012 by TUMOR.