Renal Regeneration Laboratory

North Hills, CA, United States

Renal Regeneration Laboratory

North Hills, CA, United States
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Tsurkan M.V.,Leibniz Institute of Polymer Research | Tsurkan M.V.,Center for Regenerative Therapies Dresden | Tsurkan M.V.,Renal Regeneration Laboratory | Tsurkan M.V.,University of California at Los Angeles | And 10 more authors.
Journal of Controlled Release | Year: 2013

Local delivery of growth factors (GFs) can accelerate regeneration of injured tissue, but for many medical applications, injectable GF delivery systems are required for clinical success. Viscoelastic, injectable aggregates of micrometer-sized hydrogel particles made of multiarmed polyethylene glycol (starPEG) and heparin were prepared and tested for site-specific paracrine stimulation of tissue regeneration. Heparin was used as it binds, protects and releases numerous GFs. Hydrogel based delivery of basic fibroblast growth factor (bFGF) and murine epidermal growth factor (EGF) was monitored utilizing enzyme-linked immunosorbent assay (ELISA). bFGF was released slowly because of its high affinity to the heparin while the significantly higher release of the non-specific binding EGF was controlled by diffusion only. To investigate GF delivery in vivo, a hydrogel loaded with murine EGF or bFGF was injected subcapsularly into the left kidney of mice with experimental acute kidney injury caused by glycerol induced rhabdomyolysis. Visual examination confirmed sustained stability of the injected gel aggregates during the timescale of the experiment. The number of proliferating kidney tubular epithelial cells was quantified both in the injected kidney and the non-injected contralateral kidney. bFGF delivery from hydrogels induced a significant increase in cell proliferation in the injected kidney, although small effects were also seen in the non-injected kidney due to a systemic effect. EGF delivery strongly increased cell proliferation for both kidneys, but also showed a local effect on the injected kidney. The hydrogel without loaded GFs was used as a control and showed no increase in cell proliferation. Our results suggest that this novel starPEG-heparin hydrogel system can be an effective approach to deliver GFs locally. © 2013 Elsevier B.V.

Dai J.,Auburn University | Yang X.,Auburn University | Yang X.,CA Technologies | Hamon M.,Renal Regeneration Laboratory | And 2 more authors.
Chemical Engineering Journal | Year: 2015

Controlling the particle size of semiconductors can alter their optical and electronic properties for many applications. Semiconductor nanoparticles with controlled size can be synthesized using the colloidal chemical method. In this study, we used the aqueous colloidal method to synthesize CdS nanoparticles in picoliter droplets generated by a microfluidic device. The microfluidic device used two mechanical cutting valves to generate two different droplets containing precursors (aqueous solution of cadmium chloride (CdCl2) and sodium sulfide (Na2S)). The fusion of these two droplets yielded CdS nanoparticles whose sizes were controlled by 3-Mercaptopropionic acid (MPA), a particle growth inhibitor and stabilizer. We synthesized CdS nanoparticles with narrow size distribution and investigated the effects of precursor concentration and S2-:Cd2+ molar ratio on particle growth. This microfluidic method is capable of easily tuning the reagent concentrations to synthesize nanoparticles in a highly controlled manner. © 2015 Elsevier B.V.

Rak-Raszewska A.,University of Oulu | Hauser P.V.,Renal Regeneration Laboratory | Hauser P.V.,University of California at Los Angeles | Vainio S.,University of Oulu
Stem Cells International | Year: 2015

When Clifford Grobstein set out to study the inductive interaction between tissues in the developing embryo, he developed a method that remained important for the study of renal development until now. From the late 1950s on, in vitro cultivation of the metanephric kidney became a standard method. It provided an artificial environment that served as an open platform to study organogenesis. This review provides an introduction to the technique of organ culture, describes how the Grobstein assay and its variants have been used to study aspects of mesenchymal induction, and describes the search for natural and chemical inducers of the metanephric mesenchyme. The review also focuses on renal development, starting with ectopic budding of the ureteric bud, ureteric bud branching, and the generation of the nephron and presents the search for stem cells and renal progenitor cells that contribute to specific structures and tissues during renal development. It also presents the current use of Grobstein assay and its modifications in regenerative medicine and tissue engineering today. Together, this review highlights the importance of ex vivo kidney studies as a way to acquire new knowledge, which in the future can and will be implemented for developmental biology and regenerative medicine applications. © 2015 Aleksandra Rak-Raszewska et al.

Hauser P.V.,Renal Regeneration Laboratory | Hauser P.V.,University of California at Los Angeles | Nishikawa M.,Renal Regeneration Laboratory | Nishikawa M.,University of California at Los Angeles | And 4 more authors.
Journal of Tissue Engineering and Regenerative Medicine | Year: 2016

Developmental engineering is a potential option for neo-organogenesis of complex organs such as the kidney. The application of this principle requires the ability to construct a tubular structure from dispersed renal progenitor cells with defined size and geometry. In this present study we report the generation of tubular structures from dispersed ureteric bud cells in vitro by using a micropatterned gel. Dispersed CMUB-1 cells, a mouse ureteric bud-derived cell line, or mIMCD cells, a mouse collecting duct-derived cell line, were suspended in collagen I and seeded into an agarose-based micropatterned gel. We found that within 24–36 h of incubation, the cells developed a tubular structure that conformed to the geometry of the micropattern of the gel. The lumen formation of the tubular structure was confirmed by immunohistochemical staining and observed by confocal microscopy. We found that higher concentrations of collagen I negatively influenced the efficiency of tubular formation. Tubule formation in CMUB-1, but not mIMCD, cells was positively influenced by the addition of aldosterone (10, 50 and 200 µg/ml), FGF (50 and 100 µg/ml) and fibronectin (10 and 50 µg/ml) to the growth medium. We further demonstrated the functionality of the generated tubes by in vitro budding, which was induced by growth factors, such as glial cell-derived neurotrophic factor (GDNF) or fibroblast growth factor 7 (FGF7), in the presence of beads soaked with the activin A inhibitor follistatin. Our current study thus demonstrates the possibility of constructing a functional tubular structure from dispersed ureteric bud cells in vitro in a controlled manner. Copyright © 2014 John Wiley & Sons, Ltd. Copyright © 2014 John Wiley & Sons, Ltd.

Nishikawa M.,Medical and Research Services | Nishikawa M.,University of California at Los Angeles | Nishikawa M.,Renal Regeneration Laboratory | Yanagawa N.,Medical and Research Services | And 8 more authors.
In Vitro Cellular and Developmental Biology - Animal | Year: 2013

Successful derivations of specific neuronal and glial cells from embryonic stem cells have enormous potential for cell therapies and regenerative medicine. However, the low efficiency, the complexity of induction method, and the need for purification represent obstacles that make their application impractical. In this study, we found that PDGFRα+ cells derived from mouse embryonic stem cells (mESC) can serve as a useful source from which to induce cells that express γ-aminobutyric-acid (GABA)-releasing (GABAergic) neuronal markers. PDGFRα+ cells were induced from mESC on collagen IV-coated plates in mesenchymal stem cell (MSC) culture medium with limited exposure to retinoic acid, sorted by fluorescence-activated cell sorter and maintained in MSC culture medium containing Y-27632, a Rho-associated kinase inhibitor. We found that supplementation of vascular endothelial growth factor, fibroblast growth factor-basic, and sodium azide (NaN3) to MSC culture medium effectively differentiated PDGFRα+ cells into cells that express GABAergic neuronal markers, such as Pax2, Dlx2, GAD67 NCAM, and tubulin-βIII, while markers for oligodendrocyte (Sox2) and astrocyte (Glast) were suppressed. Immunostaining for GABA showed the majority (86 ± 5%) of the induced cells were GABA-positive. We also found that the PDGFRα+ cells retained such differentiation potential even after more than ten passages and cryopreservation. In summary, this study presents a simple and highly efficient method of inducing cells that express GABAergic neuronal markers from mESC. Together with its ease of maintenance in vitro, PDGFRα+ cells derived from mESC may serve as a useful source for such purpose. © 2013 The Society for In Vitro Biology.

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