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Navi Mumbai, India

Deshpande M.S.,Reliance Life science Pvt. Ltd
Biotechnology progress | Year: 2010

A dermal tissue construct composed of human dermal fibroblasts and a chitosan sponge has been developed, targeted towards the treatment of diabetic nonhealing ulcers. The construct has been designed in a way that the dermal fibroblasts are arranged as a three-dimensional sheet adhered entirely on one side of the chitosan sponge. This design would allow maximal diffusion of growth factors from the cells to the wound bed when the construct is applied on the wound with the cellular sheet side making contact with the wound bed. The diffusion of secreted growth factors would take place directly from cells to the wound bed without being impeded by a matrix. The cells are present at a high density in the dermal construct, which would aid in accelerated wound healing. The construct has a porous chitosan sponge base, which would allow gas exchange, and renders the dermal construct very flexible so that it would take the shape of the wound contours well, while having mechanical integrity. The viability of cells in the construct is greater than 90%. The dermal construct produces a high amount of vascular endothelial growth factor, from 42 ng to 31 ng in 24 h. The construct also produces high amounts of Interleukin-8 (IL-8), from 375 ng to 1065 ng in the first 24 h. Both VEGF and IL-8 have important roles in the healing of chronic diabetic ulcers. © 2010 American Institute of Chemical Engineers

Bhatnagar R.,Reliance Life science Pvt. Ltd | Saranath D.,Reliance Life science Pvt. Ltd
Oral Oncology | Year: 2012

With a view to identify genomic risk variants in chewing-tobacco associated oral cancer patients, a genome-wide association study was conducted in patients of Indian ethnicity with long term tobacco chewing habit. We analyzed 55 oral cancer patients and 92 healthy controls for single nucleotide polymorphisms, using high throughput microarray Illumina Infinium II Assay platform and Human CNV370k-bead chip containing 370,000 single nucleotide polymorphisms. The PLINK software platform defined 298 SNPs with minor allele frequency of several genes significantly increased in oral cancer patients as compared to the controls (p < 0.001). Illumina Genome Viewer Software Version 3.2.9, further delineated 93 SNPs with p-values ranging from 9.3 × 10-4 to 1.38 × 10-5 and Odd's ratio of 2.18-8.48, associated with 70 genes. Analysis using Kyoto Encyclopedia of Genes and Genome Pathway database, indicated SNP association with several genes including GRIK2, RASGRP3, CAMK4, SYK, RAPTOR, FHIT, DCC, active in signal transduction; MMP2, CNTNAP2, PTPRJ associated with tumor cell migration; and apoptotic gene IRAK3. The data indicates an inherent role for the genetic constitution of individuals in oral carcinogenesis, with the genomic variants contributing to increased risk or susceptibility to oral cancer. © 2012 Elsevier Ltd. All rights reserved.

Viswanathan C.,Reliance Life science Pvt. Ltd | Sarang S.,Reliance Life science Pvt. Ltd
Current Diabetes Reviews | Year: 2013

Rapidly increasing number of diabetic patients across the world is a great challenge to the current therapeutic approach. Although the traditional method of rendering exogenous insulin is an established method of treatment, it is not sufficient and often causes lethal hypoglycemia. There is also a good amount of success with whole organ transplantation or Islet cells' transplantation. But this technique is limited with regards the availability of donors. Currently, many clinicians and researchers are involved in clinical studies using various different stem cells from embryonic as well as adult sources for the treatment of diabetes. In this review we have tried to discuss the results of various clinical trials using stem cells. We have also tried to look at various stem cell types and the routes of injections that are currently being followed world wide. © 2013 Bentham Science Publishers.

Johnson T.S.,Reliance Life science Pvt. Ltd | Eswaran N.,Reliance Life science Pvt. Ltd | Sujatha M.,Directorate of Oilseeds Research
Plant Cell Reports | Year: 2011

With the increase in crude oil prices, climate change concerns and limited reserves of fossil fuel, attention has been diverted to alternate renewable energy sources such as biofuel and biomass. Among the potential biofuel crops, Jatropha curcas L, a non-domesticated shrub, has been gaining importance as the most promising oilseed, as it does not compete with the edible oil supplies. Economic relevance of J. curcas for biodiesel production has promoted world-wide prospecting of its germplasm for crop improvement and breeding. However, lack of adequate genetic variation and non-availability of improved varieties limited its prospects of being a successful energy crop. In this review, we present the progress made in molecular breeding approaches with particular reference to tissue culture and genetic transformation, genetic diversity assessment using molecular markers, large-scale transcriptome and proteome studies, identification of candidate genes for trait improvement, whole genome sequencing and the current interest by various public and private sector companies in commercial-scale cultivation, which highlights the revival of Jatropha as a sustainable energy crop. The information generated from molecular markers, transcriptome profiling and whole genome sequencing could accelerate the genetic upgradation of J. curcas through molecular breeding. © 2011 Springer-Verlag.

Isar J.,Reliance Life science Pvt. Ltd | Rangaswamy V.,Reliance Life science Pvt. Ltd
Biomass and Bioenergy | Year: 2012

n-butanol fermentation by Clostridium beijerinckii ATCC 10132 was investigated by optimization of various physiological and nutritional parameters. Nitrogen source and metal ions played a crucial role on the titre of n-butanol produced in the fermentation. Optimization of the parameters resulted in about 6-fold increase and an unprecedented titre of 20gL -1 of n-butanol in 72h incubation period. The process could be scaled up to 3L level with similar titres. The strain was found to be tolerant to 25gL -1 n-butanol under optimized conditions and the tolerance could also be demonstrated by the strain's ability to accumulate rhodamine 6G. Adaptation of the strain to increased solvent in the medium was accompanied by increased expression of the chaperone, GroEL and change in fatty acid profile of total lipids. This is the first report wherein high titre of n-butanol has been obtained in single batch fermentation without resorting to solvent stripping or strain improvement. © 2011 Elsevier Ltd.

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