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Viswanathan C.,Reliance Life science Pvt Ltd | Sarang S.,Reliance Life science Pvt Ltd
Current Diabetes Reviews | Year: 2013

Rapidly increasing number of diabetic patients across the world is a great challenge to the current therapeutic approach. Although the traditional method of rendering exogenous insulin is an established method of treatment, it is not sufficient and often causes lethal hypoglycemia. There is also a good amount of success with whole organ transplantation or Islet cells' transplantation. But this technique is limited with regards the availability of donors. Currently, many clinicians and researchers are involved in clinical studies using various different stem cells from embryonic as well as adult sources for the treatment of diabetes. In this review we have tried to discuss the results of various clinical trials using stem cells. We have also tried to look at various stem cell types and the routes of injections that are currently being followed world wide. © 2013 Bentham Science Publishers.


Deshpande M.S.,Reliance Life science Pvt. Ltd.
Biotechnology progress | Year: 2010

A dermal tissue construct composed of human dermal fibroblasts and a chitosan sponge has been developed, targeted towards the treatment of diabetic nonhealing ulcers. The construct has been designed in a way that the dermal fibroblasts are arranged as a three-dimensional sheet adhered entirely on one side of the chitosan sponge. This design would allow maximal diffusion of growth factors from the cells to the wound bed when the construct is applied on the wound with the cellular sheet side making contact with the wound bed. The diffusion of secreted growth factors would take place directly from cells to the wound bed without being impeded by a matrix. The cells are present at a high density in the dermal construct, which would aid in accelerated wound healing. The construct has a porous chitosan sponge base, which would allow gas exchange, and renders the dermal construct very flexible so that it would take the shape of the wound contours well, while having mechanical integrity. The viability of cells in the construct is greater than 90%. The dermal construct produces a high amount of vascular endothelial growth factor, from 42 ng to 31 ng in 24 h. The construct also produces high amounts of Interleukin-8 (IL-8), from 375 ng to 1065 ng in the first 24 h. Both VEGF and IL-8 have important roles in the healing of chronic diabetic ulcers. © 2010 American Institute of Chemical Engineers


Hiremath A.,Reliance Life science Pvt. Ltd | Kannabiran M.,Reliance Life science Pvt. Ltd | Rangaswamy V.,Reliance Life science Pvt. Ltd
New Biotechnology | Year: 2011

The present report describes production of 1,3-propanediol by Klebsiella pneumoniae ATCC 15380 from crude glycerol from jatropha biodiesel process. Optimization resulted in a yield of up to 56. g/L of 1,3-propanediol. A conversion rate of 0.85. mol 1,3-propanediol/mol of glycerol has been obtained. Downstream processing to isolate 1,3-propanediol from the fermentation broth resulted in 99.7% pure product with a recovery of 34%. The pure 1,3-propanediol was polymerized with terephthalic acid successfully to yield polytrimethylene terephthalate. © 2010 Elsevier B.V.


Thiru M.,Reliance Life science Pvt. Ltd. | Sankh S.,Reliance Life science Pvt. Ltd. | Rangaswamy V.,Reliance Life science Pvt. Ltd.
Bioresource Technology | Year: 2011

The objective of the current report is process optimization for economical production of lipids by the well known oleaginous yeast Cryptococcus curvatus and conversion of the lipids to biodiesel. A high cell density fed-batch cultivation on low cost substrate viz. crude glycerol resulted in a dry biomass and oil yield of up to 69. g/L and 48% (w/w), respectively. The process was scaled up easily to 26. L. The oil extraction process was also optimized using environmentally safe solvents. The oil profile indicated a high oleic acid content followed by palmitic acid, stearic acid and linoleic acid. The oil was trans-esterified to biodiesel and thoroughly characterized. This is the first end to end report on production of biodiesel from the C. curvatus oil. © 2011 Elsevier Ltd.


Isar J.,Reliance Life science Pvt. Ltd. | Rangaswamy V.,Reliance Life science Pvt. Ltd.
Biomass and Bioenergy | Year: 2012

n-butanol fermentation by Clostridium beijerinckii ATCC 10132 was investigated by optimization of various physiological and nutritional parameters. Nitrogen source and metal ions played a crucial role on the titre of n-butanol produced in the fermentation. Optimization of the parameters resulted in about 6-fold increase and an unprecedented titre of 20gL -1 of n-butanol in 72h incubation period. The process could be scaled up to 3L level with similar titres. The strain was found to be tolerant to 25gL -1 n-butanol under optimized conditions and the tolerance could also be demonstrated by the strain's ability to accumulate rhodamine 6G. Adaptation of the strain to increased solvent in the medium was accompanied by increased expression of the chaperone, GroEL and change in fatty acid profile of total lipids. This is the first report wherein high titre of n-butanol has been obtained in single batch fermentation without resorting to solvent stripping or strain improvement. © 2011 Elsevier Ltd.


Varshney A.,Reliance Life science Pvt. Ltd | Johnson T.S.,Reliance Life science Pvt. Ltd
Plant Biotechnology Reports | Year: 2010

Jatropha curcas L. (Physic nut) is a commercially important non-edible oil seed crop known for its use as an alternate source of biodiesel. In order to investigate the morphogenic potential of immature embryo, explants from four developmental stages were cultured on medium supplemented with combinations of auxins and cytokinins. It was found that the size of embryo is critical for the establishment of callus. Immature embryos (1.1-1.5 cm) obtained from the fruits 6 weeks after pollination showed a good response of morphogenic callus induction (85.7%) and subsequent plant regeneration (70%) with the maximum number of plantlets (4.7/explant) on Murashige and Skoog's (MS) medium supplemented with IBA (0.5 mg l-1) and BA (1. 0 mg l-1). The above medium when supplemented with growth adjuvants such as 100 mg l-1 casein hydrolysate + 200 mg l-1l-glutamine + 8.0 mg l-1 CuSO4 resulted in an even higher frequency of callus induction (100%). Plant regeneration (90%) with the maximum number of plantlets (10/explant) was achieved on MS medium supplemented with 500 mg l-1 polyvinyl pyrrolidone + 30 mg l-1 citric acid + 1 mg l-1 BA + 0.5 mg l-1 Kn + 0.25 mg l-1 IBA. It was observed that plantlet regeneration could occur either through organogenesis of morphogenic callus or via multiplication of pre-existing meristem in immature embryos. The age of immature embryos and addition of a combination of growth adjuvants to the culture medium appear to be critical for obtaining high regeneration rates. Well-developed shoots rooted on half-strength MS medium supplemented with 0.5 mg l-1 IBA and 342 mg l-1 trehalose. The rooted plants after acclimatization were successfully transferred to the field in different agro-climatic zones in India. This protocol has been successfully evaluated on five elite lines of J. curcas. © 2010 Korean Society for Plant Biotechnology and Springer.


Johnson T.S.,Reliance Life science Pvt. Ltd | Eswaran N.,Reliance Life science Pvt. Ltd | Sujatha M.,Directorate of Oilseeds Research
Plant Cell Reports | Year: 2011

With the increase in crude oil prices, climate change concerns and limited reserves of fossil fuel, attention has been diverted to alternate renewable energy sources such as biofuel and biomass. Among the potential biofuel crops, Jatropha curcas L, a non-domesticated shrub, has been gaining importance as the most promising oilseed, as it does not compete with the edible oil supplies. Economic relevance of J. curcas for biodiesel production has promoted world-wide prospecting of its germplasm for crop improvement and breeding. However, lack of adequate genetic variation and non-availability of improved varieties limited its prospects of being a successful energy crop. In this review, we present the progress made in molecular breeding approaches with particular reference to tissue culture and genetic transformation, genetic diversity assessment using molecular markers, large-scale transcriptome and proteome studies, identification of candidate genes for trait improvement, whole genome sequencing and the current interest by various public and private sector companies in commercial-scale cultivation, which highlights the revival of Jatropha as a sustainable energy crop. The information generated from molecular markers, transcriptome profiling and whole genome sequencing could accelerate the genetic upgradation of J. curcas through molecular breeding. © 2011 Springer-Verlag.


Sharma B.P.,Reliance Life science Pvt Ltd | Saranath D.,Reliance Life science Pvt Ltd
Journal of Biosciences | Year: 2011

Germline mutations of RET gene are pathognomonic of multiple endocrine neoplasia (MEN; MEN 2A/MEN 2B) and familial medullary thyroid carcinoma (FMTC), constituting 25% of medullary thyroid carcinomas (MTCs). We investigated RET gene mutations and polymorphisms at exons 10, 11, 13, 14, 15 and 16 in 140 samples, comprising 51 clinically diagnosed MTC patients, 39 family members of patients and 50 normal individuals. The method of choice was PCR and direct nucleotide sequencing of the PCR products. RET gene mutations were detected in 15 (29.4%) patients, with MEN 2A/FMTC in 13 patients and MEN 2B in 2 patients. Further, 39 family members of seven index cases were analysed, wherein four of the seven index cases showed identical mutations, in 13 of 25 family members. We also examined single nucleotide polymorphisms (SNPs) in RET gene exons in 101 unrelated samples. Significant differences in the allelic frequencies of SNPs at codons 691, 769, 836 and 904 between patient and control groups were not observed. However, SNP frequencies were significantly different in the Indian group as compared with other European groups. We identified two novel, rare and unique SNPs separately in single patients. Our study demonstrated presence of MEN 2A/MEN 2B/FMTC-associated mutations in accordance with the reported literature. Thus, RET gene mutations in exons 10, 11, 13, 14, 15 and 16 constitute a rapid test to confirm diagnosis and assess risk of the disease in familial MEN 2A/MEN 2B/FMTC. © 2011 Indian Academy of Sciences.


Juwle A.,Reliance Life science Pvt. Ltd. | Saranath D.,Reliance Life science Pvt. Ltd.
Medical Oncology | Year: 2012

We examined BRCA1/2 mutations and single nucleotide polymorphisms (SNPs) for identification of BRCA1 haplotypes, in early-onset breast cancer patients and their relatives, sporadic breast cancer patients, and unrelated normal healthy females, of Indian ethnicity. Peripheral blood DNA was amplified by polymerase chain reaction, at BRCA1/2 coding exons and subject to nucleotide sequencing using ABI 3100 Genetic Analyzer. We observed BRCA1/BRCA2 mutations in 52 % early-onset breast cancer patients and in 57 % relatives. Deleterious mutations detected in early-onset patients and relatives were 187delAG, 632insT, 1052delT, Q759X, Q780X, R1203X, 5154delC, IVS14 + 1G > A, IVS17 + 1G > T, and 632insT in BRCA1 gene; and 4075delGT, 5076delAA, 6079delAGTT, and W3127X in BRCA2 gene. A high degree of penetrance of BRCA1/2 gene mutations was observed in the relatives. BRCA1/2 SNPs were identified in the Indian population, and association of BRCA1 haplotypes with breast cancer was investigated. A significantly increased frequency of the SNPs 203G/A, 3624A/G and 7470A/G SNPs in BRCA2 gene was observed in normal controls indicative of a protective effect of the SNPs. BRCA1 haplotype 2 was most frequently observed in our population. Our study indicates a high incidence of BRCA1/BRCA2 gene mutations in the Indian patients. The BRCA1/2 mutations and SNPs are detailed on our website http://relibrca.rellife.com. © 2012 Springer Science+Business Media, LLC.


Patent
Reliance Life science Pvt. | Date: 2010-08-11

A process for purifying hyaluronic acid (HA) or its salt from a bacterial fermentation broth comprising:(a) diluting and clarifying the fermentation broth;(b) precipitating the HA present in the broth with an equal volume of solvent;(c) dissolving the precipitated HA or its salt in a solution;(d) adding silica gel to the HA solution or HA salt solution from step (c) and then removing the silica gel;(e) treating the HA solution or HA salt solution from step (d) with active carbon; and(f) diafiltrating the HA solution or HA salt solution from step (e) using about 5 volumes of solvent;wherein the process is conducted in the absence of any detergent or surfactant or formalin, and at neutral pH.

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