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Belo Horizonte, Brazil

Freitas P.R.C.,Federal University of Minas Gerais | Coelho S.G.,Federal University of Minas Gerais | Rabelo E.,ReHagro | Lana A.M.Q.,Federal University of Minas Gerais | And 2 more authors.
Revista Brasileira de Zootecnia

Forty Holstein cows, with previous reproductive problems, empty, at second or more lactations, with an average milk production of 9,200 kg in the previous lactation and a dry period over than 50 days were distruibuted in two groups to evaluate two protocols of milking induction. It was given to group 1, on days one, eight and 21 recombinant bovine somatotropin-bSTr (500 mg); from day two to eight, estradiol cypionate (0.075 mg/kg BW) and medroxi progesterone acetate (0.25 mg/kg BW); from days nine to 15, estradiol cypionate (0.037 mg/kg BW); on day 19, prostaglandin F2α (0.530 mg) and from days 19 to 21, isoflupredone acetate (0.05 mg/kg BW). Group two was given, on days one, eight and 21, bSTr (500 mg), from days 2 to 15, estradiol benzoate (0.071 mg/kg BW), from days two to eight, acetate of medroxi progesterone (0.25 mg/kg BW), day 19, prostaglandin F2α(0.530 mg) and from days 19 to 21 isoflupredone acetate, (0.05 mg/kg BW). Teats and udders of all cows were massaged for five minutes from day 17 to 21. Milking were carried out from the 22 nd day of induction. After beginning of lactation, both groups were given bsTr (500 mg) every 14 days. It was used a complete random design with 20 replicates per treatment and a split-plot scheme. Evaluation of the protocols was carried out by using analyses of variance using Fisheŕs test at 5% and production of milking per day using regression analyses. Milking was successfully induced in 85% of the animals. Animals from group 2 showed production mean 21.9 ± 12.9 kg of milk/day which was higher than group 1 mean 18.9 ± 11.5 kg of milk/day. There was no difference for milk composition in either groups. Twenty-nine cows were inseminated and 41.1% became pregnant. © 2010 Sociedade Brasileira de Zootecnia. Source

Oliveira C.S.F.,Federal University of Minas Gerais | Oliveira C.S.F.,University Utrecht | Hogeveen H.,University Utrecht | Botelho A.M.,ReHagro | And 3 more authors.
Preventive Veterinary Medicine

Information related to mastitis risk factors is useful for the design and implementation of clinical mastitis (CM) control programs. The first objective of our study was to model the risk of CM under Brazilian conditions, using cow-specific risk factors. Our second objective was to explore which risk factors were associated with the occurrence of the most common pathogens involved in Brazilian CM infections. The analyses were based on 65 months of data from 9,789 dairy cows and 12,464 CM cases. Cow-specific risk factors that could easily be measured in standard Brazilian dairy farms were used in the statistical analyses, which included logistic regression and multinomial logistic regression. The first month of lactation, high somatic cell count, rainy season and history of clinical mastitis cases were factors associated with CM for both primiparous and multiparous cows. In addition, parity and breed were also associated risk factors for multiparous cows. Of all CM cases, 54% showed positive bacteriological culturing results from which 57% were classified as environmental pathogens, with a large percentage of coliforms (35%). Coagulase-negative Staphylococcus (16%), Streptococcus uberis (9%), S. treptococcus agalactiae (7%) and other Streptococci (9%) were also common pathogens. Among the pathogens analyzed, the association of cow-specific risk factors, such as Zebu breed (OR. = 5.84, 95%CI 3.77-10.77) and accumulated history of SCC (1.76, 95%CI 1.37-2.27), was different for CM caused by Coagulase-negative Staphylococcus and S. agalactiae in comparison to CM caused by coliforms. Our results suggest that CM control programs in Brazil should specially consider the recent history of clinical mastitis cases and the beginning of the lactations, mainly during the rainy season as important risk factor for mastitis. © 2015 Elsevier B.V. Source

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