Regional Station

Nilgiri Hills, India

Regional Station

Nilgiri Hills, India
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Singh K.M.,Karnal | Singh S.,ICAR National Bureau of Animal Genetics Resources | Ganguly I.,ICAR National Bureau of Animal Genetics Resources | Nachiappan R.K.,ICAR National Bureau of Animal Genetics Resources | And 4 more authors.
Cell Stress and Chaperones | Year: 2017

Heat stress proteins assist cellular proteins in the acquisition of native structure. The present research was conducted to study how thermo-tolerance is modulated by HSP90 and HSP70 gene polymorphism and its association with hemato-physio-biochemical parameters, supported by their expression profiles in Chokla, Magra, Marwari, and Madras Red sheep breeds. Least square analysis revealed significant effect (P < 0.05) of season and breed on all the physiological parameters, i.e., temperature, respiratory rate, and pulse rate (a.m. and p.m.), as well as hematological parameters like Hb, packed cell volume, total erythrocyte count (TEC), neutrophil/lymphocyte (N/L) ratio, and total leukocyte count (TLC). There was a significant influence (P < 0.05) of breed on biochemical parameters such as glucose, SGOT, phosphorous, triglyceride, and cholesterol. Eight fragments were amplified and sequenced in HSP90, and 70 genes and 13 single-nucleotide polymorphisms (SNPs) were identified. Tetra-primer amplification refractory mutation system PCR, PCR-RFLP, and allele-specific PCR genotyping protocols were developed for large-scale genotyping of five SNPs. A significant difference (P < 0.05) of rectal temperature (a.m.), respiratory rate (p.m.), triglyceride, and total protein was observed at SNP01; albumin at SNP2; pulse rate (p.m.) at SNP3; and rectal temperature (p.m.), pulse rate (p.m.), Hb (g/dL), and N/L ratio at SNP4 and TLC at SNP5. Gene expression analysis revealed lower expression in less adapted animals with Madras Red < Magra < Chokla < Marwari expression pattern. Predominant allele was found to be superior in most of the SNPs (SNP1–4) indicating the selection acting in directional manner (positive selection). Finally, it is concluded that TACCA haplotype combination of SNP1-SNP2-SNP3-SNP4-SNP5 might be of some selection advantage for the identification of animals more adaptable to heat stress. © 2017 Cell Stress Society International

The field experiment was conducted at PAU, Regional Station, Bathinda, for consecutive 7 years (2003-2009) to study the crop growth behavior and yield characteristics of ber fruit crop and an attempt was made to correlate the ber fruit characteristics with weather parameters over the years. The seven years data on fruit characteristics and weather parameters were taken on yearly basis and correlations were developed between ber fruit yield, fruit weight, acidity, pulp percentage, TSS and corresponding weather parameters namely minimum temperature, maximum temperature, morning relative humidity, evening relative humidity and rainfall. The regression analysis was done for four varieties of ber viz., Umran, Wailati, Chhuhara and Seb and the most suitable variety best suited for the weather conditions of southwestern region was worked out. The most sensitive period for fruit yield sensitivity appears to be during November to March months for the ber varieties under study. Different varieties responded differently with weather parameters for their different characteristics. The multiple regression models were developed on the basis of critical periods pertaining to crop yield characteristics for different varieties separately and it was found that 80-90 % variation in fruit yield and yield attributing characters were explained by weather parameters.

Bharathi L.K.,Indian Agricultural Research Institute | Vinod,Indian Agricultural Research Institute | Munshi A.D.,Indian Agricultural Research Institute | Behera T.K.,Indian Agricultural Research Institute | And 4 more authors.
Euphytica | Year: 2010

Teasle gourd [Momordica subangulata Blume subsp. renigera (G. Don) de Wilde, 2n = 56] exhibits morphological characters found in both M. dioica (2n = 28) and M. cochinchinensis (2n = 28). Morphological analysis of M. subangulata subsp. renigera suggests an allopolyploid origin. We present evidence elucidating the genomic relationships between M. dioica, M. cochinchinensis and M. subangulata subsp. renigera. A triploid M. dioica × M. subangulata subsp. renigera hybrid had an average of 12.76 bivalents, 13.84 univalents and 0.88 trivalents at metaphase I, while the M. cochinchinensis × M. subangulata subsp. renigera hybrid had an average of 13.08 bivalents, 12.96 univalents and 0.96 trivalents. F1 hybrids of the two diploid species (M. dioica × M. cochinchinensis) showed an average of 9.12 bivalents and 9.76 univalents, suggesting that the genomes of these species are only partially homologous. A higher number of bivalents in the triploid hybrids suggests that M. subangulata subsp. renigera is a segmental allopolyploid of M. dioica and M. cochinchinensis and that its genomes have diverged from the parental genomes. © 2010 Springer Science+Business Media B.V.

Bharathi L.K.,Central Horticultural Experiment Station | Munshi A.D.,Indian Agricultural Research Institute | Behera T.K.,Indian Agricultural Research Institute | John K.J.,Regional Station | And 2 more authors.
Indian Journal of Genetics and Plant Breeding | Year: 2013

Analysis of morphological data for determining the genetic variation within seven Momordica species lead to the recognition of three groups. First group containing M. charantia and M. balsamina, was characterized by n = 11, annual, monoecious, non tuberous roots and muricate - tubercled fruit surface. The second group comprised of M. dioica, M. sahyadrica, M. subangulata subsp. renigera and M. cochinchinensis which was characterized by n = 14, perennial, dioecious, tuberous tap roots and echinate-soft papillate fruit surface. The third group contained a single species M. cymbalaria and was characterized by n = 9, perennial, monoecious, tuberous tap roots, and ribbed fruit surface. The species accommodated in group I and II were similar in nature of arils, male flowers, seed surface, symmetrical anthers, ripe fruit colour and nature of epicarp while Group III could be differentiated from other two groups in these characters. The range of intra-specific variation, particularly for fruit and seed characters, was high in all the species studied. At inter-specific level, wide variation was observed for all the quantitative traits studied. Extensive collection, characterization, evaluation for resistance to biotic/abiotic stresses and conservation of species diversity assumes great priority in crop improvement programmes of Momordica.

Bharathi L.K.,Central Horticultural Experiment Station | Singh H.S.,Central Horticultural Experiment Station | John K.J.,Regional Station
Genetic Resources and Crop Evolution | Year: 2014

A new synthetic species of Momordica (2n = 56) has been developed by crossing natural tetraploid Momordica subangulata subsp. renigera (2n = 56) with induced tetraploid Momordica dioica (2n = 4x = 56). The hybrid produces adventitious root tubers through which it perpetuates and propagates like its female parent and maintains its morphological characteristics in the progeny. The hybrid is naturally fertile and has the superior agronomic traits of both parents making it a good choice as a new vegetable crop. The new species is named Momordica × suboica Bharathi and described. © 2014 Springer Science+Business Media Dordrecht.

Roy S.,Regional Station | Verma S.K.,Regional Station | Hore D.K.,Regional Station | Hore D.K.,Krishidhan Seeds | And 3 more authors.
Indian Journal of Agricultural Sciences | Year: 2011

Eightythree turmeric accessions from five states of north-eastern India were compared under high humid sub-tropics at mid altitude (1 000 m elevation) for ten quantitative characters using multivariate methods, including clustering and principal component analysis to assess the patterns of morphological variation. Plant height, leaf number, primary fingers size and suckers number showed positive and significant association with rhizome yield. The accessions were grouped into four clusters and within each group, morphological variations were of low order. The first four principal components contributed 75% of the variability. Both clustering and principal component analysis have proved to be effective methods in grouping turmeric accession according to their morphological characteristics and better understanding of the existing variability that will facilitate genotypic selection for crop improvement.

Bhardwaj S.C.,Directorate of Wheat Research | Prashar M.,Directorate of Wheat Research | Kumar S.,Directorate of Wheat Research | Sharma Y.P.,Directorate of Wheat Research | And 2 more authors.
Cereal Research Communications | Year: 2010

Two new pathotypes of Puccinia triticina , 121R60-1 and 377R60-1 the latter virulent on Lr28 , are described for the first time. Both the pathotypes are designated as MHTTS as per North American system of pathotype identification. Pathotype 377R60-1 appears to be the result of a single step mutation for virulence to Lr28 in pathotype 121R60-1. Both pathotypes appear closely related to the most prevalent virulent pathotype 121R63-1(THTTS). The avirulence/virulence profile, resistance sources and their possible relationship with other pathotypes are discussed. Lr28 is now included as one of the differentials for the identification of leaf rust pathotypes. © 2010 Akadémiai Kiadó.

Raina A.P.,National Bureau of Plant Genetic Resources | Negi K.S.,Regional Station
Journal of Herbs, Spices and Medicinal Plants | Year: 2012

The chemical composition of essential oils of two species of Lavendula - Lavendula stoechas and L. angustifolia - from India was investigated by gas chromatography and gas chromatography/mass spectrometry. The essential oil yield from the flowering spikes were 0.86% in L. stoechas and 1.56% in L. angustifolia. Twenty-five components amounting to 96.7% of the oil were identified in L. stoechas, and 31 compounds were identified in L. angustifolia, representing 91.7% of the oil. The major compounds present in the oil of L. stoechas were camphor (52.1%), fenchone (12.0%), 1,8-cineole (9.7%), bornyl acetate (6.2%), camphene (3.3%), α-pinene (1.1%), and terpinen-4-ol (0.6%), and the oil of L. angustifolia exhibited linalool (23.6%), linalyl acetate (35.8%), α-terpinole (6.3%), lavendulyl acetate (4.8%), 1,8-cineole (1.5%), terpinen-4-ol (2.0%), β-caryophyllene (1.8%), camphor (1.4%), and borneol (1.4%). Results showed that the essential oils of these Lavendula sp. were rich in oxygenated monoterpenes ranging from 86.3% in L. stoechas to 82.6% in L. angustifolia and can be exploited for various applications. Copyright © Taylor & Francis Group, LLC.

Prasad P.,Regional Station | Pandey S.,Arid forest Research Institute | Kumar J.,Govind Ballabh Pant University of Agriculture & Technology
Indian Journal of Natural Products and Resources | Year: 2015

Isothiocyanates, the degradation products of glucosinolates reported specifically in the members of Brassicaceae family are potential alternative compounds to currently used fumigants. In the present study, six Brassica species, viz. Brassica alba (L.) Rabenh, B. nigra (L.) W. D. J. Koch (cv. ‘Banarasi rai’), B. napus L. (cv. ‘PPNS-1’), B. rapa L. (cv. ‘RESBR-240’), B. juncea (L.) Czern. (cv. ‘Kranti’) and B. carinata A. Braun (cv. ‘Kiran’) were evaluated for Sinigrin (one of the most important glucosinolates found in Brassica) content using High Performance Liquid Chromatography. Sinigrin concentration in different Brassica species ranged between 3.65 μM/g in B. napus L. to 16.42 μM/g in B. juncea (L.) Czern. (cv. ‘Kranti’). Therefore, B. juncea (L.) Czern. (cv. ‘Kranti’) was found as the most appropriate Brassica species to be used as biofumigant followed by B. nigra (L.) W. D. J. Koch (cv. ‘Banarasi rai’) and B. alba (L.) Rabenh. © 2015 National Institute of Science Communication and Information Resources (NISCAIR). All right reserved.

Pandravada S.R.,Regional Station | Varaprasad K.S.,Regional Station | Janardhan Reddy K.,Regional Station | Janardhan Reddy K.,Osmania University | Rao E.S.,Regional Station
Indian Journal of Agricultural Sciences | Year: 2010

Out of 172 accessions of chilli germplasm artificially screened against Meloidogyne javanica in the glass house conditions, the root-knot nematode infestation was observed in 161 aces, including the check varieties. The reaction of the germplasm varied from highly resistant to highly susceptible with the per cent gall index (PGI) ranging from 0 to 100. A total of 11 entries were found to be highly resistant, 3 entries as moderately susceptible, 36 entries as susceptible and 122 entries as highly susceptible. Conspicuously, there were no entries that reacted under moderately resistant or resistant categories in the germplasm. In the present investigation, a total of 11 accessions found free from the incidence of root-knot originated from India, Taiwan and Hungary were identified as promising. Out of these, 'EC 378632' and 'EC 402113' which were also found to be highly resistant to dieback and sunscald and having significant yield potential are sources for multiple resistance in chilli improvement programmes.

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