Da O.,Regional Research Institute for Health science ICES DRO CNRST |
Da O.,Bobo-Dioulasso Polytechnic University |
Traore-Coulibaly M.,Regional Research Institute for Health science ICES DRO CNRST |
Traore-Coulibaly M.,Center Muraz |
And 11 more authors.
American Journal of Biochemistry and Molecular Biology | Year: 2014
Saye is an antimalarial recipe containing Cochlospermum planchonìì Hook. F. (Cochlospermaceae), Phyllanthus amarus Schumach and Thonn (Euphorbiaceae) and Cassia alata h. (Fabaceae). This study assessed the antiplasmodial and antioxidant activity of the aqueous extracts of the individual plants and their combinations. Extracts were assessed on Plasmodium berghei infected mice according to the 4 day suppressive test, for their total phenol contents according to Folin-Ciocalteu method and their antioxidant potential by FRAP assay and by the inhibition of ion induced lipid peroxidation in rat liver homogenate. Macerated extracts from Cochlospermum planchonii, Phyllantus amarus and Cassia alata gave 50.6, 46.3 and 44.9% inhibition of the parasites at 100 mg kg-1 body weight (b.wt.), respectively. At 250 mg kg-1 b.wt. the decocted extract of Cochlospermum planchoii gave 54% inhibition. Decocted extracts of the combinations Cassia alata+Phyllanthus amarus (2:1) and Phyllanthus amarus+Cochlospermum planchonii (2:1) reduced mice parasitemia by about 20-30% at 100 mg kg-1 b.wt. At the same dose, a 4.9-15.9% inhibition was observed with the decocted extract of Cassia alata+Cochlospermum planchonii (1:1) and the whole "Saye" but a higher effect of 43.7 to 50.3% was observed at 250 mg kg-1 b.wt. Phyllantus amarus extract exhibited the highest total phenol content (294 jig TAEs/mg). The highest content in flavonoids 43 jig QE/mg and the highest inhibition of lipid peroxidation (22.56%) were found for Cassia alata+Phyllanthus amarus (2:1) and the best reducing power (41.38 jimol TE/mg) for Saye. Saye appears as promising antioxidant and could be used as preventive agent in oxidative stress diseases such as malaria. © 2014 Academic Journals Inc.