Regional Plant Resource Center

Orissa, India

Regional Plant Resource Center

Orissa, India
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Nayak A.K.,Regional Plant Resource Center | Babu B.K.,Regional Plant Resource Center
Journal of Plant Pathology | Year: 2017

The bottle gourd (Lagenaria siceraria, family Cucurbitaceae) is a medicinal plant (Sirohi and Sivakami, 1991) widely cultivated throughout India. In December 2014, powdery mildew symptoms were observed on L. siceraria in different fields of the Odisha state (India), i.e. circular white mycelial patches 1 to 2 mm in diameter on the upper surface of the leaves, which coalesced and developed into larger circular or irregular spots on both leaf surfaces. Infected leaves dried and eventually dropped. Conidiophores were 110-220 × 11-13.5 μm in size and produced 3 to 5 immature conidia in chains with a crenate outline. Foot cells were 40-75 μm long, straight, cylindrical, slightly constricted at the basal septum. Conidia were hyaline, ellipsoid-ovoid, 25-40 × 17-22 μm in size and had distinct fibrosin bodies. These morphological traits suggested this fungus to be a species of the genus Podosphaera, likely corresponding to Podosphaera xanthii (Braun and Cook, 2012). For confirmation, the internal transcribed spacer (ITS) region of rDNA from conidia was amplified with primers ITS 1/ ITS 4 and sequenced according to Babu et al. (2015). The resulting 182 bp sequence (GenBank accession No. KU376473) was analysed by BLAST homology search against GenBank database revealing 100% similarity with P. xanthii (KX061106, KR779870). Pathogenicity was determined by inoculating conidial suspension onto young leaves of five healthy potted L. siceraria in a greenhouse at 25-28°C (> 80% humidity). Five non inoculated plants served as control. Symptoms like those shown in the field developed 5-7 days post inoculation only on inoculated plants from which P. xanthii was reisolated. To our knowledge, this is the first report of P. xanthii as the cause of powdery mildew disease on bottle gourd in India. © 2017, Edizioni ETS. All rights reserved.


Dhal Y.,Regional Plant Resource Center | Deo B.,Regional Plant Resource Center | Sahu R.K.,Bjb Auto College
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2012

Curcuma zedoaria (Christm.) belongs to family Zingiberaceae shows active antioxidant enzymes like catalase, guaiacol peroxidase, glutathione peroxidase and superoxide dismutase which have various biological activities. The enzymatic leaf extracts of this plant have been analyzed for their free radical-scavenging activity in different in vitro systems, e.g. DPPH free radical scavenging activity, hydroxyl free radical-scavenging activity and total antioxidant activity. The free radical scavenging activities were compared with standard antioxidant like ascorbic acid. The DPPH radical scavenging activity was found to be 39.7± 0.2 with 200 μg/ml of the enzymatic extract. Total antioxidant activity was measured and it was found to be 121±0.7(mg AE /g) and 91.3 ± 0.5 (mg TE/g) with 100 μl of the enzymatic extract of leaf samples. The maximum antioxidant activities were found in catalase, glutathione peroxidase and superoxide dismutase enzyme where as the guaiacol peroxidase shows poor antioxidant activity as compared to ascorbic acid. The hydroxyl radical scavenging activity was found to be 48.23±0.05 with 50 μl of the enzymatic extract. Therefore, it is suggested that C. zedoaria could be a potential source of natural antioxidant that could have great importance in therapeutic agent in preventing or slowing down the progressive ageing and age associated oxidative stress related degenerative diseases.


Aparajita S.,Regional Plant Resource Center | Rout G.R.,Orissa University of Agriculture and Technology
Zeitschrift fur Naturforschung - Section C Journal of Biosciences | Year: 2011

Phyllanthus has been widely used in traditional medicine as an antipyretic, a diuretic, and to treat liver diseases and viral infections. Correct genotype identification of medicinal plant material remains important for the botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated the need for newer methods in quality control of botanicals. In the present study, attempts were made to identify species- diagnostic markers for ten Phyllanthus species using the inter simple sequence repeat-polymerase chain reaction (ISSR-PCR) fingerprinting method. PCR amplification using seven ISSR primers resulted in significant polymorphism among the populations from different species. P. angustifolius and P. urinaria showed monomorphic frequency of maximum (63.88%) and minimum (20.64%), respectively. Seventeen species-diagnostic markers were identified for seven species (P. acidus, P. emblica, P. fraternus, P. urinaria, P. rotundifolius, P. amarus, and P. angustifolius) while no marker was detected for P. reticulatus, P. nivosus, and P. virgulatus. A maximum of six species-diagnostic markers were identified for P. acidus and a minimum of only one of 755 bp was available for P. amarus. Among the seventeen markers, nine were present in all individuals of particular species. The speciesspecific differences in fragment numbers and sizes could be used as diagnostic markers to distinguish the Phyllanthus species quickly. © 2011 Verlag der Zeitschrift für Naturforschung.


Palai S.K.,Regional Plant Resource Center | Rout G.R.,Regional Plant Resource Center
Horticultura Brasileira | Year: 2011

Chrysanthemum is the important cut flower after rose among the ornamental plants traded in the global flower market. It is propagated vegetatively and also has a strong sporophytic self-incompatibility system as shown by all members of Asteraceae family. Morphologically, the petal numbers and flower colours present maximum variation when compared to existing varieties. Twenty Inter Simple Sequence Repeat primers were used to detect the new variety of Chrysanthemum developed through spontaneous sporting. The results indicate that the rate of polymorphism showed significant differences as compared to other existing varieties. The average number of amplification products per primer was eight. The size of ISSR amplified fragments varied from 0.25 - 2.4 Kbp. Therefore, ISSR marker is a useful technique for the rapid and easy assessment of genetic variation among the variants. Morphological traits of new variants showed variation as compared to other parents. The 1st flower bud appearance and the height of 1st bud of the variant were less as compared to original mother variety. The new variants can be propagated in large scale commercially through in vitro technique.


Mahapatra A.K.,Regional Plant Resource Center | Mishra S.,Regional Plant Resource Center | Basak U.C.,Regional Plant Resource Center | Panda P.C.,Regional Plant Resource Center
Advance Journal of Food Science and Technology | Year: 2012

Considering the growing need to identify alternative bio-nutritional sources, 15 wild edible fruits consumed in deciduous forest zone of India were evaluated for their nutritive value in order to prioritize edible wild fruits suitable for domestication. The result showed significance of wild fruit species as important source of nutrient for rural poor. The nutritional value of many wild fruits compared well with domesticated popular fruits as mango, banana, guava, papaya, sapota, pomegranate, strawberry etc. in terms of protein, carbohydrate or Vitamin content. The carbohydrate content in wild varieties as Mimusops elengi (18.1%) is found to be at par with mango (17%) and pomegranate (17.1%). High concentration of sugar was noted in Ziziphus rugosa (20.7%) compared to domesticated sapota (21.4%), grapes (16.2%) and pomegranate (16.5%). Protein content in Bridelia tomentosa (3.1%), Carissa spinarum (3.6%) and Polyalthia suberosa (1.9%) was found similar to cultivated fruits, viz., guava (2.5%), banana (1.09%) and lemon (1.1%). Maximum proportion of Ascorbic acid/Vitamin-C content was seen in case of Solanum torvum (37.4 mg/100 g), Terminalia citrina (53.52 mg/100 g), which is higher to banana (8.7 mg/100 g), apple (4.6 mg/100 g), pomegranate (6.1 mg/100 g) and mango (27.7 mg/100 g). Of particular importance are Eugenia rothii, Mimusops elengi, Ziziphus oenoplia, Ziziphus rugosa, Bridelia tomentosa and Carissa spinarum that had significant level of micronutrient and minerals and therefore were identified as promising species for promotion as backyard planting especially farming systems suffering from crop loss, food shortage and chronic malnutrition. © Maxwell Scientific Organization, 2012.


Mahapatra A.K.,Regional Plant Resource Center | Panda P.C.,Regional Plant Resource Center
Food Security | Year: 2012

A number of wild plants, used by rural and tribal populations and contributing significantly to their livelihood and food security have escaped recognition and scientific inquiry. Their distribution, conservation, mode of harvest by locals and optimal use require region-specific assessment in order to integrate them into developmental interventions. This study analyzed the collection, consumption, sale and income from edible forest fruits in 49 tribal villages spread over five districts of Orissa State in eastern India. Density, dominance and diversity of species yielding wild fruit were measured by studying ecological parameters in the sample plots. We estimated an average of 48 fruit plants per hectare of deciduous forests. Fifty-six wild edible fruit species belonging to 40 genera in 26 families were recorded in the study region, many of which have multiple uses. Indigenous fruits formed part of the family diet with average annual consumption of 73 kg per household. Sale of wild fruits contributed 15 % of income for tribal households. Despite their good knowledge of indigenous fruits, the tribal populations have not adopted fruit tree farming which would enhance their nutrition and income. © 2012 Springer Science + Business Media B.V. & International Society for Plant Pathology.


Sahoo D.P.,Regional Plant Resource Center | Samantrai D.,Regional Plant Resource Center | Rout G.R.,Regional Plant Resource Center
Plant Biosystems | Year: 2011

An efficient protocol was developed for in vitro clonal propagation of Saccharum officinarum Vars. CO-6907 and CO-86249 through axillary meristem culture. Maximum meristem elongation was achieved on Murashige and Skoog's (MS) medium supplemented with 0.5 mg/L 6-benzyladenine (BA) and 0.5 mg/L kinetin (Kn) within 15 days of culture. Multiple shoots were induced from meristems on MS basal medium supplemented with 1.0 mg/L BA, 0.5 mg/L Kn, 0.25 mg/L 1-napthaleneacetic acid (NAA) and 3% (w/v) sucrose. Addition of 0.1-0.25 mg/L gibberellic acid into the multiplication medium found the better shoot elongation. Repeated subculture on multiplication medium induces higher rate of shoot multiplication. The root induction from excised microshoots was achieved on half-strength MS medium supplemented with 1.0-2.0 mg/L NAA or indole-3-butyric acid and 6% (w/v) sucrose. While either decreasing or increasing of sucrose concentration in the rooting medium, the percentage of rooting was reduced. Maximum percentage of rooting was achieved on medium having 2.0 mg/L NAA with 6% (w/v) sucrose. About 80% of micropropagated plantlets were hardened in the greenhouse and successfully established in the soil. Random Amplified Polymorphic DNA marker was used to detect the variability among the micropropagated plants developed through in vitro. The results showed that there was no polymorphism among the micropropagated plants. This study will help for propagation of quality planting material of highyielding variety of sugarcane for commercialization. © 2011 Società Botanica Italiana.


Mahapatra A.K.,Regional Plant Resource Center | Shackleton C.M.,Regional Plant Resource Center
Forest Policy and Economics | Year: 2011

State control of marketing agricultural or forestry products may lead to a trade monopoly and restricted competition, resulting in inequitable returns to producers. Consequently, market deregulation for agriculture and other natural products has been advocated to improve market efficiencies, participation and returns for the producers and local traders. The study examines the impact of deregulation of state marketing controls on collection, sale and returns to extractors and traders from non-timber forest products. It assessed whether deregulation policy resulted in greater benefits to primary extractors and a win-win situation for both producers and traders? Mixed effects were found, with no significant changes in terms of trade, demand, or quantum of consumption; but there was an overall increase in the procurement price for several products following deregulation. The collection and farm gate price of tree based oilseeds and honey increased due to lower transaction costs, and ease of trading with a continued demand for these products. The impact on less valuable products was insignificant. Abolition of monopolistic trading increased competition and had a pull effect on the local market prices of several NTFPs. Although traders paid a higher price to procure products, overall they also benefited being able to sell the purchased products to merchants and manufacturers at a better margin than previously. © 2011 Elsevier B.V.


Gupta N.,Regional Plant Resource Center | Sahoo D.,Regional Plant Resource Center | Basak U.C.,Regional Plant Resource Center
African Journal of Microbiology Research | Year: 2010

Phosphate solubilising ability of five Streptomyces isolated from the phyllosphere of Heritiera fomes grown in Bhitarkanika mangrove ecosystem was evaluated using tricalcium phosphate (TCP) in both plates and broth culture conditions as well as with and without NaCl. Streptomyces ST24 showed highest solubilization of TCP, with 50.8 and 48.0 mm of halo zone in the plate assay done at pH 7.2 and temperatures 30 and 37°C. Streptomyces ST21, ST24 and ST26 showed good solubilization of TCP in culture medium with 52.15, 50.77 and 52.07 μg/ml, respectively. The requirement of NaCl for better solubilization of TCP was observed in all Streptomyces. However, ST23 and ST24 showed solubilization activity without addition of NaCl. Thus the solubilization potential varies among different isolates of Streptomyces. It also differed according to incubation period. Over all, the best solubilization ability of all test Streptomyces could be observed in the presence of 0.2% NaCl. The solubilization might be due to production of acids by the culture, since the pH of the culture broth was changed from initial pH of 7.2 and 9.0 to lower pH values. © 2010 Academic Journals.


Sahoo D.P.,Regional Plant Resource Center | Aparajita S.,Regional Plant Resource Center | Rout G.R.,Regional Plant Resource Center
Plant Systematics and Evolution | Year: 2010

Pongamia pinnata is an oil-producing tree species with multiple uses and considerable potential as a bioenergy crop. This investigation was carried out to assess the extent of genetic structure in a representative set of 226 individuals of Pongamia pinnata encompassing seven populations as a prelude to utilization of promising and genetically divergent material in breeding programmes. Molecular polymorphism was 67.18% with ten inter simple sequence repeats (ISSR) between the individuals indicating modest levels of genetic variation in the Pongamia pinnata germplasm collected. The within-population variation based on ISSR polymorphism was 32.34% and polymorphism at the species level was 94.3%. Genetic differentiation between populations (GST = 0.61) was positively correlated with geographical distance. The data obtained indicate an immediate need to widen the genetic base of Pongamia pinnata germplasm for proper characterization, and for extensive plantations of elite varieties to meet the demands for biodiesel. © Springer-Verlag 2009.

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