Aparajita S.,Regional Plant Resource Center |
Rout G.R.,Orissa University of Agriculture and Technology
Zeitschrift fur Naturforschung - Section C Journal of Biosciences | Year: 2011
Phyllanthus has been widely used in traditional medicine as an antipyretic, a diuretic, and to treat liver diseases and viral infections. Correct genotype identification of medicinal plant material remains important for the botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated the need for newer methods in quality control of botanicals. In the present study, attempts were made to identify species- diagnostic markers for ten Phyllanthus species using the inter simple sequence repeat-polymerase chain reaction (ISSR-PCR) fingerprinting method. PCR amplification using seven ISSR primers resulted in significant polymorphism among the populations from different species. P. angustifolius and P. urinaria showed monomorphic frequency of maximum (63.88%) and minimum (20.64%), respectively. Seventeen species-diagnostic markers were identified for seven species (P. acidus, P. emblica, P. fraternus, P. urinaria, P. rotundifolius, P. amarus, and P. angustifolius) while no marker was detected for P. reticulatus, P. nivosus, and P. virgulatus. A maximum of six species-diagnostic markers were identified for P. acidus and a minimum of only one of 755 bp was available for P. amarus. Among the seventeen markers, nine were present in all individuals of particular species. The speciesspecific differences in fragment numbers and sizes could be used as diagnostic markers to distinguish the Phyllanthus species quickly. © 2011 Verlag der Zeitschrift für Naturforschung.
Patra A.P.,Orissa University of Agriculture and Technology |
Mukherjee A.K.,Indian Council of Agricultural Research |
Acharya L.,Regional Plant Resource Center
American Journal of Biochemistry and Molecular Biology | Year: 2011
In Orissa Betel vine (Piper betle L., family Piperaceae) is an important asexually propagated cash crop comprising of several cultivars. There are many cultivares but they are not well demarcated due to similarities in the morphological characters and in certain places same cultivars are cultivated under different local name. Therefore, in the present study DNA fingerprinting technique has been used to differentiate cultivars of betel vine for crop improvement programme. So Comparative study of both RAPD and ISSR markers analysis were used to establish genetic identities and evaluate genetic diversity among fifteen cultivars of betel vine grown in different parts of Orissa. Thirty RAPD and 25 ISSR primers were tested to resolve the genetic diversity among the cultivars. Twenty RAPD and 18 ISSR primers resulted in 523 amplicons. Out of these 504 were polymorphic loci and 54 were found to be unique. The extent of genetic diversity and relatedness among 15 cultivars were computed through Jaccard's similarity coefficient. Maximum similarity (0.68) was observed between Balipana and Birkoli and minimum (0.114) for Banglamandesore chitalpudi and Halisahar Sanchi. All the cultivars were related with each other with an average similarity of 0.2913. Dendrogram showed Godibangala was separated from rest of the species into isolated clade in both the analysis. Correlation between RAPD and ISSR marker was very low (r = 0.17). RAPD showed high correlation with all the primers. © 2011 Academic Journals Inc.
Palai S.K.,Regional Plant Resource Center |
Rout G.R.,Regional Plant Resource Center
Horticultura Brasileira | Year: 2011
Chrysanthemum is the important cut flower after rose among the ornamental plants traded in the global flower market. It is propagated vegetatively and also has a strong sporophytic self-incompatibility system as shown by all members of Asteraceae family. Morphologically, the petal numbers and flower colours present maximum variation when compared to existing varieties. Twenty Inter Simple Sequence Repeat primers were used to detect the new variety of Chrysanthemum developed through spontaneous sporting. The results indicate that the rate of polymorphism showed significant differences as compared to other existing varieties. The average number of amplification products per primer was eight. The size of ISSR amplified fragments varied from 0.25 - 2.4 Kbp. Therefore, ISSR marker is a useful technique for the rapid and easy assessment of genetic variation among the variants. Morphological traits of new variants showed variation as compared to other parents. The 1st flower bud appearance and the height of 1st bud of the variant were less as compared to original mother variety. The new variants can be propagated in large scale commercially through in vitro technique.
Separation of the genera in the subtribe Cassiinae (Leguminosae: Caesalpinioidae) using molecular markers [Separaçao dos gêneros na subtribo Cassiinae (Leguminosae: Caesalpinioidae) utilizando marcadores moleculares).]
Acharya L.,Siksha O' Anusandhan University |
Mukherjee A.K.,Regional Plant Resource Center |
Panda P.C.,Regional Plant Resource Center
Acta Botanica Brasilica | Year: 2011
Random amplified polymorphic DNA (RAPD), Inter simple sequence repeat (ISSR) and Amplified fragment length polymorphism (AFLP) markers were used to verify the segregation of the genus Cassia L. senso lato into three distinct genera namely Chamaecrista Moench., Senna P. Mill. and Cassia L. sensostricto Eighteen representatives of the three taxa were characterized using the molecular markers. 25 RAPD, six ISSR primers and six AFLP primer combinations resulted in the amplification of 612, 115 and 622 bands (loci) respectively. Most of the loci are found to be polymorphic, showing high degrees of genetic diversity among the different taxa studied. The dendrogram constructed on the basis of the RAPD, ISSR and AFLP data using SHAN clustering, divided Cassia L. senso lato. into three different clusters as Chamaecrista Moench. Senna P. Mill. and Cassia L. senso stricto High bootstrap value revealed that all the clusters were stable and robust. It was observed from the present investigation that these genera have their identity at molecular level, which supports the elevation of the genus Cassia L. senso lato to the level of subtribe Cassiinae and segregation into three distinct genera instead of intrageneric categories.
Mahapatra A.K.,Regional Plant Resource Center |
Panda P.C.,Regional Plant Resource Center
Food Security | Year: 2012
A number of wild plants, used by rural and tribal populations and contributing significantly to their livelihood and food security have escaped recognition and scientific inquiry. Their distribution, conservation, mode of harvest by locals and optimal use require region-specific assessment in order to integrate them into developmental interventions. This study analyzed the collection, consumption, sale and income from edible forest fruits in 49 tribal villages spread over five districts of Orissa State in eastern India. Density, dominance and diversity of species yielding wild fruit were measured by studying ecological parameters in the sample plots. We estimated an average of 48 fruit plants per hectare of deciduous forests. Fifty-six wild edible fruit species belonging to 40 genera in 26 families were recorded in the study region, many of which have multiple uses. Indigenous fruits formed part of the family diet with average annual consumption of 73 kg per household. Sale of wild fruits contributed 15 % of income for tribal households. Despite their good knowledge of indigenous fruits, the tribal populations have not adopted fruit tree farming which would enhance their nutrition and income. © 2012 Springer Science + Business Media B.V. & International Society for Plant Pathology.