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Miron I.,Grigore T. Popa University of Medicine and Pharmacy | Miron I.,Sfanta Maria Emergency Childrens Hospital | Moisa S.M.,Grigore T. Popa University of Medicine and Pharmacy | Moisa S.M.,Sfanta Maria Emergency Childrens Hospital | And 10 more authors.
Journal of B.U.ON. | Year: 2014

Purpose: To evaluate the incidence and time of occurrence of chemotherapy-related toxic events in 100 children admitted to the Hematology-Oncology Ward of "Sfanta Maria" Children's Emergency Hospital in Iasi, Romania, over a 4-year period. Methods: An analytical, descriptive and comparative, retrospective and prospective study covering a 4-year period on the incidence of chemotherapy side effects, was performed on 100 children admitted for solid tumors or hematologic malignancies. The probability of each adverse event to appear and the time period from chemotherapy initiation to the moment of side effect appearance were assessed. Results: The most frequent toxicity was alopecia (79.5%), followed by medullary aplasia (71.1%), oral candidiasis (65.3%), diarrhea and emesis (64% each), toxic hepatitis (61%), and Cushing's syndrome (21.5%). Oral herpes and thrush were less frequent (13.2% and 12.2%, respectively). Remissions of the underlying disease were achieved in 69.9% of the cases. Alopecia, medullary aplasia and oral candidiasis developed during the first 14 months of treatment. Mucositis, emetic syndrome and toxic hepatitis were diagnosed within the first 12 months of treatment. Diarrhea and oral herpes or thrush appeared during the first 15 months, while Cushing's syndrome developed during the first 6 months. All remissions were obtained during the first 4 months of treatment. Conclusions: While alopecia and medullary aplasia were the most frequent side effects of chemotherapy in our study group, the earliest were Cushing's syndrome, emetic syndrome and toxic hepatitis.


Costache I.-I.,Grigore T. Popa University of Medicine and Pharmacy | Costache I.-I.,Sf Spiridon Emergency Districtual Clinical Hospital | Aprotosoaie A.C.,Grigore T. Popa University of Medicine and Pharmacy | Ivanov I.C.,Grigore T. Popa University of Medicine and Pharmacy | And 6 more authors.
Biomedical Research (India) | Year: 2015

Clopidogrel resistance is a phenomenon which has clinical implications related to the evolution and unfavorable prognosis of patients with acute coronary syndromes (ACS). Although different mechanisms for Clopidogrel resistance have been suggested, genetic polymorphism of liver enzyme CYP2C19 is on of the most prominent. The aim of the study was to determine variations in response to Clopidogrel in a group of 96 patients diagnosed with acute coronary syndromes (ACS), by genotyping the CYP2C19 cytochrome and the ABCB1 gene. CYP2C19 and ABCB1 polymorphisms were investigated using two methods: TaqMan Assay and High Resolution Melting (HRM) technique. Depending on the genotypic constellation of the alleles studied for CYP2C19 gene, patients were divided into several prediction categories: normal metabolizer (6.25%); intermediate metabolizer (0%); slow metabolizer (17.70%); very fast metabolizer (60.41%) and unpredictable metabolizer (15.62%). HRM technique may be a viable alternative to determine the CYP2C19*2, CYP2C19*17 and ABCB1 polymorphisms, being less expensive than TaqMan Assay. © 2014, Biomedical Research. All rights reserved.


Lucaci L.,Grigore T. Popa University of Medicine and Pharmacy | Lucaci L.,Institute Of Cardio Vascular Diseases Prof Dr G Georgescu | Moisa S.M.,Grigore T. Popa University of Medicine and Pharmacy | Moisa S.M.,Sfanta Maria Emergency Childrens Hospital | And 4 more authors.
Revista Romana de Medicina de Laborator | Year: 2014

Introduction: Methotrexate, a structural analogue to the folic acid, is one of the most frequently used antimetabolites in pediatric oncologic pathology. Its mode of action and toxic effects are now well known. Material and method: Our study aimed to describe the quantitation of the drug in serum of 40 children with acute lymphatic leukemia receiving high doses of methotrexate and to predict serum methotrexate levels at 96 hours, based on 48 h, 72h levels and on alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea, and creatinine serum levels. The above mentioned parameters were analyzed by sampling serum levels at 48h, 72h and 96 hours after methotrexate administration and a logical regression model being projected upon obtained results. Results and conclusions: methotrexate serum level at 96 hours does not depend on either AST, ALT, urea, creatinine levels or on the methotrexate level determined at 48 hours, it only depends on the methotrexate serum level at 72 hours.


Mihalas E.,Grigore T. Popa University of Medicine and Pharmacy | Matricala L.,Petru Poni Institute of Macromolecular Chemistry | Chelmus A.,Regional Oncology Institute | Chelmus A.,Grigore T. Popa University of Medicine and Pharmacy | And 4 more authors.
Toxicologic Pathology | Year: 2016

Amoxicillin used in early childhood may be associated with enamel hypomineralization. Our aim was to assess disturbances of amelogenesis in mice lower incisors induced by chronic administration of amoxicillin/clavulanic acid (AMC). Twenty-eight C57BL/6 male mice, of similar age, randomly divided into a control and 3 treatment groups (n = 7) received subcutaneous injection, once per day, for 60 days: 50, 100, and 150 mg/kg BW of AMC. Scanning electron microscopy/energy dispersive X-ray spectroscopy analysis in AMC treatment groups showed higher content in F and a decrease in P and Ca. Morphology changes ranged from scratched patterns, and small isolated pits-like enamel loss, to generalized demineralized enamel surface, giving a rough, foamy, scaly, or even cracked eggshell appearance to the affected areas. Histological analysis showed disturbances of maturation ameloblasts, which were less organized, with increased amounts of clear vacuoles in the cytoplasm and slightly more elongated and less condensed nucleus. Additionally, they were often detached from the enamel matrix. Transitional ameloblasts formed underlying the cysts of varied sizes. In conclusion, AMC dose-dependently affect ameloblast functions especially in the maturation phase, causing hypomineralized enamel formation with quantitative and/or qualitative defects. © The Author(s) 2015.


Scripcariu D.V.,Grigore T. Popa University of Medicine and Pharmacy | Scripcariu S.-I.,Grigore T. Popa University of Medicine and Pharmacy | Ciorap R.,Grigore T. Popa University of Medicine and Pharmacy | Scripcariu V.,Grigore T. Popa University of Medicine and Pharmacy | Scripcariu V.,Regional Oncology Institute
2015 E-Health and Bioengineering Conference, EHB 2015 | Year: 2015

Anorectal manometry is one way of evaluating the patient's quality of life, as it is an efficient way of assessing the anal function before and after neoadjuvant and/or surgical treatment. It is important to evaluate the function of the anal sphincter, as it can dictate the surgical treatment that is applied to the patient. The main issue that arises is the large variability of results, related to the operator-linked bias, as well as the different types of equipment that are used. Standardization is required. The studies in literature lack homogeneity, as some studies show there is a link between neoadjuvant therapy and anal incontinence, whereas others say there is no relation, while others even show the benefic effect on the short term of neoadjuvant radiation therapy on anal sphincter function. There is also the issue of long-term results and follow-up. In the instance of a "sphincter-saving" intervention, evaluation of the tonicity of the anal sphincter is important, as it can dictate between choosing a colorectal/coloanal anastomosis and realizing a terminal colostomy. © 2015 IEEE.


Negura L.,Grigore T. Popa University of Medicine and Pharmacy | Dusa C.P.,Sf Maria Clinical Emergency Hospital For Children | Balmus M.I.,Al. I. Cuza University | Azoicai D.,Grigore T. Popa University of Medicine and Pharmacy | And 5 more authors.
Romanian Journal of Morphology and Embryology | Year: 2015

Developed two decades ago, oncogenetic medical practice mainly concern breast, ovarian and colorectal cancers, and is targeting the hereditary risk factor, the only one that shows positive predictive value justifying the molecular diagnosis. Screening for BRCA1 and BRCA2 gene mutations is standard practice today for hereditary breast and ovarian cancer (HBOC) families in developed countries, offering the possibility of medical follow-up. The gold standard for molecular diagnosis is Sanger sequencing of all exons and exon-intron boundaries, which is expensive and time consuming. More than 3000 BRCA sequence variants are reported in international databases, but in some populations or ethnic groups a few founder mutations showed to have a recurrent presence. This may be very useful in establishing a combined technical approach for mutation detection, including rapid and cheap pre-screening methods for most common mutations. The BRCA1 5382insC mutation has an Ashkenazi founder effect and is also the second most recurrent mutation in Eastern European populations, having been already identified in several Romanian HBOC patients. Here we present a complete screening of consecutive series of breast and ovarian cancer patients for the presence of BRCA1 5382insC. The presence of the mutation was investigated by allele specific multiplex- PCR on genomic DNA extracted from peripheral blood. No mutation carrier was identified among breast or ovarian cancer patients. Our findings suggest that BRCA1 5382insC may not have a strong recurrent effect in Romanian population comparing to neighboring countries. This may be particularly useful in establishing further pre-screening strategies. © 2015, Editura Academiei Romane. All rights reserved.


Mihalache G.,Al. I. Cuza University | Zamfirache M.-M.,Al. I. Cuza University | Mihasan M.,Al. I. Cuza University | Ivanov I.,Regional Oncology Institute | And 2 more authors.
Archives of Biological Sciences | Year: 2015

Soil microorganisms, especially rhizobacteria, play a key role in soil phosphorus (P) dynamics and the subsequent availability of phosphate to plants. Utilization of phosphate-solubilizing bacteria as biofertilizers instead of synthetic chemicals is known to improve plant growth through the supply of plant nutrients, and may help to sustain environmental health and soil productivity. The main purpose of this study was to identify new phosphate-solubilizing bacteria isolated from runner bean rhizosphere. Ten out of 25 isolated bacterial strains solubilized Ca3(PO4)2 in qualitative and quantitative P-solubilization. The strain that exhibited the highest potential to solubilize Ca3(PO4)2, was selected for further determination of the mechanisms involved in the process. The medium pH was measured, organic acids released in the culture medium were identified by HPLC analysis, and the acid and alkaline phosphatase activities were determined. Our results showed that strain R7 solubilized phosphorous through the production of various organic acids such as lactic, isocitric, tartaric and pyruvic acids, and that it can be used as a potential biofertilizer.

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