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Mueang Nonthaburi, Thailand

Waneesorn J.,Regional Medical science Center | Panyasai S.,University of Phayao | Kongthai K.,Health Promoting Hospital | Singboottra P.,Chiang Mai University | Pornprasert S.,Chiang Mai University
Hemoglobin | Year: 2011

Hb Constant Spring [Hb CS; α142, Term→Gln (TAA>CAA in α2)] is often missed by routine laboratory testing since its mRNA as well as gene product are unstable and presented at a low level in peripheral blood. This study aimed to analyze the efficacy of capillary electrophoresis (CE) and high performance liquid chromatography (HPLC) for detecting and quantifying Hb CS in 19 heterozygotes and 14 homozygotes with Hb CS as well as 10 Hb H-CS disease subjects who were detected by molecular analysis. In the CE electrophoregram, Hb CS was seen at zone 2 and was observed in all samples, while the chromatogram of Hb CS peaks was found in 26.32% heterozygotes, 42.86% homozygotes and 90% Hb H-CS disease subjects, respectively. In addition, the Hb CS levels in each group of subjects quantified by CE were significantly higher than those quantified by HPLC. Based on the CE method, the lowest Hb CS level was found in the heterozygotes, whereas the highest level was found in the Hb H-CS disease patients. Therefore, the CE method was superior to the HPLC method for detecting Hb CS. Furthermore, the level of Hb CS quantified by CE proved useful in screening heterozygotes and homozygotes with Hb CS as well as Hb H-CS disease. © 2011 Informa Healthcare USA, Inc.

Pornprasert S.,Chiang Mai University | Panyasai S.,University of Phayao | Waneesorn J.,Regional Medical science Center | Kongthai K.,Health Promoting Hospital Chiang Mai | Singboottra P.,Chiang Mai University
Clinical Laboratory | Year: 2012

Background: Gel-electrophoresis and ethidium bromide are not ideally suited to large scale analysis in clinical laboratories. Methods: Amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) specific for Hb CS was performed in 10 blood samples from normal individuals and 61 samples containing a peak of Hb CS when analyzed by capillary electrophoresis. Heterozygosity of Hb CS was identified using SYTO9 and high resolution melting (HRM) analysis method. Results: Specific peak heights of amplified fragments of wild type and Hb CS alleles were observed in the heterozygote. Only one peak height of amplified fragments of the wild type allele was observed in the normal individual while only one peak height of amplified fragments of Hb CS allele was observed in the homozygote. HRM analysis interpretation results were completely consistent with the interpretation results from gel-electrophoresis. Conclusions: SYTO9 HRM analysis may be used as an alternative for rapid diagnosis of heterozygosity of Hb CS.

Kunkaew W.,Royal Project Foundation | Julsrigival S.,Royal Project Foundation | Tipparat P.,Regional Medical science Center | Pinmanee S.,Highland Research and Development Institute Public Organization
Sabrao Journal of Breeding and Genetics | Year: 2011

Selection for reduced Δ 9-tetrahydrocannabinol (THC) content in four Thai hemp cultivars (including V50, Mae Sa Mai, Huay Hoi and Pang Ung) was carried out in highland areas in the northern Thailand. Research work was conducted for two consecutive growing seasons during 2008 to 2009 at Pangda Royal Agricultural Station, Samoeng district, Chiang Mai province, Thailand. Results of selection indicated that after selecting for two successive generations, the average THC content of four Thai hemp cultivars reduced to 18.0-55.0% and cannabidiol (CBD) content increased to 20.0-127.0%. The results of selection also indicated that chemotype classification could be grouped by using the ratio of CBD/THC content as follows: non-drug type (CBD/THC>10.0), intermediate type (1.0≤CBD/THC≤10.0) and drug type (CBD/THC<1.0). Thus, selection for reduced THC content, high ratio of CBD/THC content could be used as alternative criteria for improving low THC content in hemp cultivars. As well, mass selection method was considered as an effective and suitable method for improving these THC and CBD traits.

Pornprasert S.,Chiang Mai University | Waneesorn J.,Regional Medical science Center
Hemoglobin | Year: 2013

A capillary electrophoresis (CE) method has been proven to be superior to a high performance liquid chromatography (HPLC) method in the detection of Hb H-Constant Spring/Paksé [Hb H (β4) Hb CS or α142, Term→Gln, TAA>CAA (α2)]/Paksé [α142, Term→Tyr, TAA>TAT (α2)]. It also has the ability to quantify Hb Bart's (γ4). The aim of this study is to analyze the efficacy of the CE and HPLC in the detection of Hb H (β4)-CS/Paksé-E [β26(B8)Glu→Lys, GAG>AAG] disease. The laboratory results from July 2009 to July 2012 were reviewed at the Thalassemia Laboratory of the Associated Medical Sciences Clinical Service Center, Chiang-Mai University, Chiang-Mai, Thailand. The HPLC or CE method was used for the diagnosis of β-thalassemia (β-thal) and hemoglobinopathies, and molecular analysis was used for the diagnosis of α-thalassemia-1 (α-thal-1) Southeast Asian (SEA) and Thai type deletions, Hb CS and Hb Paksé. Hb H-CS-E was found in six samples and Hb H-Paksé-E was found in one sample, respectively. On the capillary electrophoregram, peaks of Hb Bart's and Hb CS/Paksé were observed in all samples with the mean levels at 2.4 and 1.0%, respectively. These peaks were also presented on the HPLC chromatogram. However, the Hb CS/Paksé level could be quantified in only three of these seven (43.0%) samples. Therefore, CE was proven to be superior to HPLC in the detection of Hb H-CS/Paksé-E disease, which will assist in diagnostic, counseling and prevention programs for these diseases. © 2013 Informa Healthcare USA, Inc.

Vestergaard M.,Technical University of Denmark | Cavaco L.M.,Technical University of Denmark | Sirichote P.,Regional Medical science Center | Unahalekhaka A.,National Institute of Health | And 4 more authors.
Frontiers in Microbiology | Year: 2012

Methicillin resistant Staphylococcus aureus (MRSA) have emerged among livestock in several countries. In this study, we describe the results of a screening performed in pigs and raw pork samples in Thailand. Ten pork samples and 15 nasal swabs from pigs were collected from 2 markets and 1 pig farm in the Samuth Songkhram province inThailand. MRSA were isolated using selective isolation procedures and confirmed by mecA PCR.The MRSA were characterized by antimicrobial susceptibility testing, pulsed field gel electrophoresis (PFGE), spa typing, SCCmec typing, and MLST Resistance and virulence markers were screened using a microarray. Five of the pork samples and six pig nasal swabs were positive for MRSA. All 11 isolates belonged to spa type t337 but showed diversity in antimicrobial resistance patterns and PFGE profiles. Additionally, the isolates were sequence-typed; ST9, ST2136, ST2278 belonging to the clonal complex; CC9. All isolates harbored SCCmec IX and were resistant to 7 out of 14 tested antimicrobials; additional resistances to all antimicrobials tested were found in some of the pork and pig isolates and 1 pork isolate was resistant to 13 antimicrobials tested. Microarray analysis identified blaZ, aacaphD, vga(A), tetM, and a tet efflux marker, in all strains and additionally ermB and aadD, cat and fex(A) in the pork isolates. None of the isolates were found PVL-positive, but enterotoxins were identified in all isolates. To our knowledge, only a few descriptions of MRSA in livestock and food products inThailand have been observed but this is the first observation of MRSA CC9 associated with SCCmec IX in pork. This study indicates a likely widespread distribution of MRSA in pig and pork inThailand and further investigation on the prevalence and importance of livestock associated MRSA inThailand is needed. © 2012 Vestergaard, Cavaco, Sirichote, Unahalekhaka, Dangsakul, Svendsen, Aarestrup and Hendriksen.

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