Regional Medical Research Center Region

Dibrugarh, India

Regional Medical Research Center Region

Dibrugarh, India
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Khan S.A.,Regional Medical Research Center Region | Kakati S.,Assam Medical College & Hospital | Dutta P.,Regional Medical Research Center Region | Chowdhury P.,Regional Medical Research Center Region | And 6 more authors.
Indian Journal of Medical Research | Year: 2016

Background & objectives: Japanese encephalitis (JE) caused by mosquito-borne Flavivirus is one of the leading causes of viral encephalitis in Asia. Control strategies include vector control and human vaccination. Due to lack of immunization programmes in endemic regions, there are still high mortality and morbidity. A live-attenuated SA 14-14-2 JE vaccine (LAJEV) has been licensed and used in Asian countries, including India. We report the assessment of immunogenicity and safety of the vaccine in adults during the first mass adult vaccination campaign carried out in Assam, India. Methods: One thousand and seventy five adults (aged ≥15 yr) who received LAJEV were monitored for adverse events following immunization for one year. The safety assessment of vaccinated population was evaluated till 28 days and at 6 and 12 months. Blood samples collected from the enrolled participants were tested by plaque reduction neutralization test (PRNT50) to assess the neutralizing antibody titres (NATs) before vaccination and 28 days, six and 12 months post-vaccination (PV). Results: Among the 1075 vaccinated individuals, four reported minor adverse effects from 30 min to 28 days PV. Based on the pre-vaccination NAT, the study participants were categorized as seronegative, moderately seropositive and strongly seropositive. Nearly 85.5 per cent of JE seronegative participants seroconverted by 28 days PV. The geometric mean titre (GMT) in all the three groups increased by 28 days and decreased by six and 12 months PV. Nearly 60 per cent of the moderately positive individuals exhibited four-fold rise in GMT, 28 days PV. Almost 95.5 per cent of the participants in the study population remained seroprotected at the end of 12 months PV. Interpretation & conclusions: This study on immunogenicity and safety of LAJEV in adults showed that a single dose of the live-attenuated vaccine was safe and induced protective immunity to both JE seronegative and naturally seropositive adults. Further study is required to find out long term protective efficacy of this vaccine. © 2017, Indian Council of Medical Research. All rights reserved.


Gahtori P.,Uttarakhand Technical University | Gahtori P.,Dibrugarh University | Ghosh S.K.,Dibrugarh University | Parida P.,Dibrugarh University | And 4 more authors.
Experimental Parasitology | Year: 2012

Present communication deals with the docking study of hybrid phenyl thiazolyl-1,3,5-triazine analogues (1a-36d) on three selected different binding site viz., α, β and γ of wild type Pf-DFHR-TS. In admiration of excellent H-bond scoring, with regard to cycloguanil and to a large extent similar scoring with WR99210, compound 4a, 12b, 21c, 23c, 28d, 29d, 34d, and 35d were selected for in vitro antimalarial activity against 3D7 strain of Plasmodium falciparum. Findings from the study disclose that a significant correlation was exist between in vitro results and in silico prediction (r 2=0.543). Furthermore, investigation of structure-activity relationships elucidate crucial structural requirement for site specific binding of ligands. © 2012 Elsevier Inc.


Paine S.K.,Regional Medical Research Center Region | Mondal L.K.,Regional Institute of Ophthalmology | Borah P.K.,Regional Medical Research Center Region | Bhattacharya C.K.,Regional Medical Research Center Region | Mahanta J.,Regional Medical Research Center Region
Molecular Vision | Year: 2017

Purpose: Alteration of pro- and antiangiogenic homeostasis of vascular endothelial growth factor (VEGF) isoforms in patients with hyperglycemia seems crucial but substantially unexplored at least quantitatively for diabetic retinopathy (DR). Therefore, in the present study we aimed to estimate the difference between the pro- (VEGF165a) and antiangiogenic (VEGF165b) VEGF isoforms and its soluble receptors for severity of DR. Methods: The study included 123 participants (diabetic retinopathy: 81, diabetic control: 20, non-diabetic control: 22) from the Regional Institute of Ophthalmology, Kolkata. The protein levels of VEGF165a (proangiogenic), VEGF165b (antiangiogenic), VEGF receptor 1 (VEGFR1), VEGFR2, and VEGFR3 in plasma were determined with enzyme-linked immunosorbent assay (ELISA). Results: An imbalance in VEGF homeostasis, a statistically significant concomitant increase (p<0.0001) in the level of VEGF165a and a decrease in the level of VEGF165b, was observed with the severity of the disease. Increased differences between VEGF165a and VEGF165b i.e. VEGF165a-b concomitantly increased statistically significantly with the severity of the disease (p<0.0001), patients with diffuse diabetic macular edema (DME) with proliferative DR (PDR) had the highest imbalance. The plasma soluble form of VEGFR2 concentration consistently increased statistically significantly with the severity of the disease (p<0.0001). Conclusions: The increased difference or imbalance between the pro- (VEGF165a) and antiangiogenic (VEGF165b) homeostasis of the VEGF isoforms, seems crucial for an adverse prognosis of DR and may be a better explanatory marker compared with either VEGF isoform. © 2017 Molecular Vision.


Dohutia C.,Regional Medical Research Center Region | Mohapatra P.K.,Regional Medical Research Center Region | Bhattacharyya D.R.,Regional Medical Research Center Region | Gogoi K.,Regional Medical Research Center Region | And 2 more authors.
Journal of Parasitic Diseases | Year: 2017

To reduce the dependency on fresh AB+ serum in continuous culture of Plasmodium falciparum, a comparative study was undertaken to assess the in vitro adaptability of P. falciparum to media supplemented with fresh AB+ serum from whole blood, AB+ plasma, serum derived from AB+ plasma, AB+ human serum from Sigma, Albumax II, fetal bovine serum and new born calf serum, independently and in different combinations. Combinations were used to analyze whether two different substitutes demonstrate any synergistic effect on the growth of the parasites. Our findings exhibited that the combination of fresh human serum and Albumax II showed good growth pattern in comparison to that of fresh serum and can thereby be instrumental in reducing the role of fresh human serum in continuous parasite maintenance. Culture maintained with Albumax II with or without hypoxanthine showed average growth. © 2016, Indian Society for Parasitology.


Gahtori P.,Uttarakhand Technical University | Pandey R.,Uttarakhand Technical University | Kumar V.,Uttarakhand Technical University | Ghosh S.K.,Dibrugarh University | And 6 more authors.
Journal of Chemometrics | Year: 2016

The front-line antimalarial drugs, for example, chloroquine, mefloquine, sulfadoxine, pyrimethamine, atovaquone, and artemether, are often failing because of the worldwide spread of drug-resistant parasites. There has been significant recent interest in virtual screening to drive innovative drug discovery and to combat resistance efforts for a wide range of diseases. In fact, virtual screening has become the “gold standard” for major pharmaceutical industries and some university groups. Therefore, we present herein a structure-based LibDock/CHARMM modeling and a set of appropriate scoring function evaluation criteria: correlation, consensus score, correlation-based score, generally applicable replacement for root-mean-square deviation using a training set of 38 phenylthiazolyl-1,3,5-triazines from our previous reports and followed by a ligand-based model to identify molecular features like hydrogen-bond acceptor, hydrogen-bond donor, hydrophobicity, and ring aromatic (RA) using Catalyst HipHop/HypoGen module. Next, TOPKAT module was applied to predict ADME-Tox properties. The combined structure/ligand-based approaches inadvertently arrived at a conserved Arg122 binding site from reliable LigScore1_dreiding top scoring function and are subsequently attributed to reserve important interactions and combat mutational drug resistance. The best pharmacophore model suggested that 1 hydrogen-bond acceptor, 2 hydrophobicities, and 1 ring aromatic feature with good sensitivity at 0.50, specificity at 0.66, enrichment at 1.60, and accuracy at 0.50. Finally, good pharmacokinetics, metabolic stability, and toxicity endpoints were predicted in the comparison of proguanil and cycloguanil. These druggability insights are useful for researchers to deliver more effective, safer, both wild-type and resistance-compromised, and more economical dihydrofolate reductase inhibitors in the near future. Copyright © 2016 John Wiley & Sons, Ltd.


Dutta P.,Regional Medical Research Center Region | Khan S.A.,Regional Medical Research Center Region | Khan A.M.,Regional Medical Research Center Region | Sharma C.K.,Regional Medical Research Center Region | Mahanta J.,Regional Medical Research Center Region
Journal of the American Mosquito Control Association | Year: 2010

A mosquito faunistic survey conducted during 19992005 in 7 different states of the northeastern (NE) region of India revealed that the region is very rich in mosquito fauna, particularly of the genus Aedes. From the present survey and earlier published reports, a total of 48 species of Aedes and a related genus Verrallina were recorded. The state of Assam contributed the highest number of species compared to the other 6 states, namely Arunachal Pradesh, Manipur, Meghalaya, Mizoram, Nagaland, and Tripura. Larvae of 46 species were collected from 16 different habitats and for some species the habitats were species specific. The known dengue vectors, Aedes aegypti and Ae. albopictus, were recorded from all 7 states, although Ae. aegypti was not known earlier from Mizoram, Nagaland, and Tripura states. Aedes albotaeniatus was a new addition to the Aedes fauna of the region. © 2010 by The American Mosquito Control Association, Inc.


PubMed | Regional Medical Research Center Region
Type: Journal Article | Journal: Journal of the American Mosquito Control Association | Year: 2010

A mosquito faunistic survey conducted during 1999-2005 in 7 different states of the northeastern (NE) region of India revealed that the region is very rich in mosquito fauna, particularly of the genus Aedes. From the present survey and earlier published reports, a total of 48 species of Aedes and a related genus Verrallina were recorded. The state of Assam contributed the highest number of species compared to the other 6 states, namely Arunachal Pradesh, Manipur, Meghalaya, Mizoram, Nagaland, and Tripura. Larvae of 46 species were collected from 16 different habitats and for some species the habitats were species specific. The known dengue vectors, Aedes aegypti and Ae. albopictus, were recorded from all 7 states, although Ae. aegypti was not known earlier from Mizoram, Nagaland, and Tripura states. Aedes albotaeniatus was a new addition to the Aedes fauna of the region.

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