Regional Forensic Science Laboratory

Rājkot, India

Regional Forensic Science Laboratory

Rājkot, India
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Nagesh K.R.,Father Muller Medical College | Menezes R.G.,Srinivas Institute of Medical science | Rastogi P.,Manipal University India | Naik N.R.,Regional Forensic Science Laboratory | And 3 more authors.
Journal of Forensic and Legal Medicine | Year: 2011

Colchicum autumnale is commonly known as autumn crocus, and as 'gowri gedde' in the southern region of Karnataka State in South India. It contains an alkaloid called colchicine, which blocks the cell division by inhibiting mitosis. We present a sporadic case of suicidal plant poisoning wherein a 24-year-old man consumed 'gowri gedde' to end his life. Initially he presented with severe vomiting, diarrhoea and epigastric pain. He died on the third day of ingestion due to multiorgan failure. Chemical analysis of blood and viscera obtained postmortem confirmed the presence of colchicine. Colchicine poisoning is potentially life threatening because of its high toxicity and unavailability of specific antidotal treatment. It classically presents with gastroenterocolitis, and may result in multiorgan failure in fatal cases. © 2011 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.

Agrawal N.,Dr Hari Singh Gour University | Esteve-Romero J.,Jaume I University | Bose D.,Dr Hari Singh Gour University | Dubey N.P.,Regional Forensic Science Laboratory | And 2 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2014

Citalopram, paroxetine and fluoxetine are selective serotonin reuptake inhibitor (SSRIs) currently used in the treatment of psychiatric disorders. We present an analytical method using micellar liquid chromatography to quantify these three drugs in pharmaceutical formulations, plasma and urine. The resolution was performed using a mobile phase of 0.075M SDS - 6% (v/v) butanol buffered at pH 7 running through a C18 column under isocratic mode at 1mL/min at 25°C. The analytes were eluted in less than 20min. The fluorescence detection was programmed at the maximum excitation (236, 295 and 230nm) and emission (310, 350 and 305nm) wavelengths for citalopram, paroxetine and fluoxetine, respectively. The experimental procedure was expedited to 1/5 dilution of the sample in the micellar mobile phase and filtration, thus avoiding clean-up and extraction steps. An aliquot of 20μL was injected after 80min of preparation, to obtain maximum sensitivity. The method was validated according to the guidelines of the Food and Drug Administration (FDA) in terms of calibration range (20-500ng/mL; r2>0.999), sensitivity, accuracy (91.3-103.2%), precision (<9.3%), and robustness (<6.1%). The suitability of the method was successfully evaluated by analyzing plasma and urine samples from patients treated with SSRIs and checking the content of the active principle in tablets. Thus, the method can be applied to pharmacokinetics studies and in forensic cases, as well as in quality control of commercial pharmaceutical formulations. © 2014 Elsevier B.V.

Dhingra V.,Regional Forensic Science Laboratory | Dhingra S.,P.A. College | Singla A.,Bundelkhand University
Egyptian Journal of Forensic Sciences | Year: 2013

The Bark of Terminalia arjuna is considered as Cardio protective and Hypolipidemic in folklore medicine. In the present investigation, the detailed pharmacognostic study of T. arjuna Bark is carried out to lay down the standards, which could be useful in future Forensic identification of unknown plant material. The study includes macroscopic, microscopic, preliminary phytochemical screening and physicochemical evaluation. The objective of this study was to characterize the unknown plant material seized from the scene of crime. In the present communication, a TLC-method was also described for the identification of Arjuna bark. © 2012 Forensic Medicine Authority.

Sankar R.,Bharathidasan University | Sankar R.,Sungkyunkwan University | Shivashangari K.S.,Regional Forensic Science Laboratory | Ravikumar V.,Bharathidasan University
RSC Advances | Year: 2016

Over the past few centuries, interest in novel metal nanoparticles has expanded quickly with the inclusion of new nanocomposites into a variety of products and technologies. Recently, nanocomposites prepared by the distribution of inorganic nanoparticles in polymeric matrices have found a prominent role in biomedical applications. In this report, we attempt to formulate a biodegradable poly-d,l-lactide-co-glycolide (PLGA) polymer based biogenic silver nanocomposite using a modified solvent casting method. The silver nanoparticles mediated by Origanum vulgare aqueous leaf extract (Ag NPs) and formulated silver polymeric nanocomposite (PLGA/Ag NC) show absorbance spectra at 420 nm under UV-visible spectroscopy. Fourier transform infrared spectroscopy confirms that Ag NPs utilized the carbonyl functional group present in the PLGA polymer for the successful formation of PLGA/Ag NC. Scanning electron microscopy analysis notably showed that PLGA/Ag NC was found to be spherical and irregular in shape, distributed in the polymer matrix. A dynamic light scattering measurement showed that PLGA/Ag NC has an average particle size distribution of 115 nm, and the zeta potential was -33 mV. The PLGA/Ag NC crystalline nature was confirmed using X-ray diffraction analysis. Furthermore, PLGA/Ag NC improves wound healing in the excision wound which was established using wound closure, histopathology, protein profiling, and matrix metalloproteinases 2 and 9 expression. The analysis results clearly show that PLGA/Ag NC enhances the wound healing activity by the sustained release of Ag NPs, upregulates protein expression and inhibits pathogenic bacterial growth in the wound area. In conclusion, PLGA/Ag NC could be a novel therapeutic agent for wound treatment. © The Royal Society of Chemistry 2016.

Sankar R.,Bharathidasan University | Dhivya R.,Bharathidasan University | Shivashangari K.S.,Regional Forensic Science Laboratory | Ravikumar V.,Bharathidasan University
Journal of Materials Science: Materials in Medicine | Year: 2014

The titanium dioxide nanoparticles (TiO2·NPs) were synthesized utilizing Origanum vulgare under room temperature. The green synthesized TiO2 NPs excitation was confirmed using UV-Vis spectrophotometer at 320 nm. Scanning electron microscopy analysis showed TiO2·NPs are spherical in shape and connected with one another. Dynamic light scattering analysis results specified high stability in nanoparticles, with an average particle size of 341 nm. Fourier transform infrared spectroscopy peaks revealed the presence of bioactive functional groups in Origanum vulgare aqueous leaf extract much needed for the TiO2·NPs formation. X-ray diffraction spectra showed the TiO2·NPs are amorphous in nature. Furthermore, the green synthesized TiO2·NPs wound healing activity was examined in the excision wound model by measuring wound closure, histopathology and protein profiling, revealed significant wound healing activity in Albino rats. In conclusion, our results bared TiO2·NPs have delivered a novel therapeutic route for wound treatment in clinical practice. © 2014 Springer Science+Business Media.

Sankar R.,Bharathidasan University | Manikandan P.,Bharathidasan University | Malarvizhi V.,Bharathidasan University | Fathima T.,Bharathidasan University | And 2 more authors.
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | Year: 2014

Copper oxide (CuO) nanoparticles were synthesized by treating 5 mM cupric sulphate with Carica papaya leaves extract. The kinetics of the reaction was studied using UV-visible spectrophotometry. An intense surface Plasmon resonance between 250-300 nm in the UV-vis spectrum clearly reveals the formation of copper oxide nanoparticles. The results of scanning electron microscopy (SEM) and dynamic light scattering (DLS) exhibited that the green synthesized copper oxide nanoparticles are rod in shape and having a mean particle size of 140 nm, further negative zeta potential disclose its stability at -28.9 mV. The Fourier-transform infrared (FTIR) spectroscopy results examined the occurrence of bioactive functional groups required for the reduction of copper ions. X-ray diffraction (XRD) spectra confirmed the copper oxide nanoparticles crystalline nature. Furthermore, colloidal copper oxide nanoparticles effectively degrade the Coomassie brilliant blue R-250 dye beneath the sunlight. © 2013 Elsevier Ltd. All rights reserved.

Sankar R.,Bharathidasan University | Karthik A.,Bharathidasan University | Prabu A.,Bharathidasan University | Karthik S.,Bharathidasan University | And 2 more authors.
Colloids and Surfaces B: Biointerfaces | Year: 2013

In the present study, we achieved silver nanoparticles using the aqueous extract of Origanum vulgare (Oregano) by reducing 1mM silver nitrate (AgNO3) solution. The green synthesized silver nanoparticles were characterized by high throughput techniques like UV-vis spectroscopy, Fourier infrared spectroscopy (FT-IR), field emission-scanning electron microscopy (FE-SEM), X-ray diffraction (XRD) and dynamic light scattering measurements. Morphologically, the nanoparticles were found to be spherical with an average particle size distribution of 136±10.09nm. FT-IR spectral analysis illustrates the occurrence of possible biomolecules required for the reduction of silver ions. The obtained nanoparticles were stable (-26±0.77mV) at ambient temperature. The biosynthesized nanoparticles were found to be impressive in inhibiting human pathogens. The green synthesized silver nanoparticles showed dose dependent response against human lung cancer A549 cell line (LD50 - 100μg/ml). © 2013 Elsevier B.V.

Kumar R.,Regional Forensic Science Laboratory | Patial N.,State Forensic Science Laboratory | Singh S.,State Forensic Science Laboratory
Journal of Forensic Sciences | Year: 2013

Linkage of a cutting tool (a sickle) with a telephone cable of 100 pairs of copper wires is reported in a case of theft of a telephone cable. Telephone cables contain numerous insulated copper wires of small diameter inside an outer covering and are stolen for the relatively precious copper. The cutting pattern of the cable can only point to the type of the tool but because of the large number of copper wires, it is practically difficult to make a definite linkage with the tool used to cut the cable by comparing the tool marks. The present work reports the successful attempt of the authors in establishing a definite linkage of a stolen telephone cable with a sickle recovered from the suspects. Spot microchemical test was performed for detection of copper on the blade of the sickle, and then tool mark comparison was performed to link the tool marks on the metal strip inside the outer covering of the cable to the blade of the sickle. © 2012 American Academy of Forensic Sciences.

Pathan A.M.,Regional Forensic Science Laboratory | Baseer M.A.,Yeshwant Mahavidyalaya | Junne S.B.,Yeshwant Mahavidyalaya
Journal of Planar Chromatography - Modern TLC | Year: 2016

The insecticides are broadly divided into organophosphates, organochlorines, carbamates, and pyrethroids. Various chromogenic spray reagents have been reported for the identification of these classes of pesticides and insecticides. Some of them are specific and some are general for a particular class of insecticides. The large volume of insecticides belong still to the organophosphates which can be divided into thio and non-thio insecticides, on the basis of the presence or absence of a sulfur atom. We here present alkaline sodium nitroprusside as a simple chromogenic spray reagent which reacts with only a specific type of organothiophosphorus insecticides and not all organothiophosphorus insecticides. © Akadémiai Kiadó.

Rawat B.R.,Regional Forensic Science Laboratory
Indian Journal of Forensic Medicine and Toxicology | Year: 2012

Postmortem, ante mortem blood samples and gastric contents in a case of poisoning by consuming homemade liquor (gudanji) were analyzed to ascertain the volatiles responsible for poisoning. Head Space-Gas Chromatography (HS-GC) highly sensitive technique was used for separation of volatiles and quantitative estimation of ethyl alcohol and methyl alcohol. Gas Chromatograph Clarus 500 coupled with Turbo matrix Head space sampler with gases of highest purity from gas generators Ptl were used in the study. Maximum concentration of ethyl alcohol (689.0871mg %) was observed in the gastric content and methyl alcohol (98.7445mg %) in ante mortem blood of subject-2. Attempt was made to identify the volatiles and to compare the chromatogram of volatiles with chromatogram of water extract of empty container recovered by police from the shed where subjects has consumed home made illicit liquor (gundanji).

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