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Unlusayin M.,Akdeniz University | Hadaruga N.G.,Banats University of Agricultural science and Veterinary Medicine King Mihai I of Romania Timisoara | Rusu G.,Polytechnic University of Timisoara | Gruia A.T.,Regional Center for Immunology and Transplant | And 2 more authors.
LWT - Food Science and Technology | Year: 2016

European anchovy (Engraulis encrasicolus L.) is one of the most important oily fish that provide valuable omega-3 containing fish oil. The aim of the study was to protect the labile fatty acids from anchovy oil by β-cyclodextrin complexation. The competitiveness to molecular encapsulation of anchovy oil components were also studied. Anchovy oil/β-cyclodextrin complexes at molar ratios of 1:1 and 1:3 were obtained by crystallization and kneading methods with very good recovering yields (74-78% and 85-99%, respectively). The most concentrated fatty acids in the raw anchovy oil were PUFAs at a relative concentration of 65.5 g/100 g. Higher content of EPA and DHA of 28.2 and 22.3 g/100 g had determined. Molecular encapsulation competitiveness conduct to a decrease of the PUFAs (even by five to seven times for EPA and DHA) and a significant increase of saturated fatty acids in complexes. Thermogravimetry and differential scanning calorimetry parameters well correlate with Karl Fischer titration parameters. They also supports the formation of inclusion compound by means of lowering the water content that is replaced by anchovy oil components. As a conclusion, valuable anchovy oil/β-cyclodextrin complexes as well as omega-3 concentrated anchovy oil (non-encapsulated "residue") can be obtained in one step for food applications. © 2015 Elsevier Ltd.

Moleriu R.D.,West University of Timisoara | Zaharie D.,West University of Timisoara | Moatar-Moleriu L.C.,West University of Timisoara | Moatar-Moleriu L.C.,Victor Babes University of Medicine and Pharmacy Timisoara | And 3 more authors.
Journal of Theoretical Biology | Year: 2014

T-cells develop in the thymus and based on CD4 and CD8 expressions there are four main thymocyte populations in a normal mouse thymus. Currently, there are several mathematical models that describe the dynamics of thymocyte populations in a normal thymus, but only a few of them model the transient perturbation of their homeostasis. Our aim is to model the perturbation in the dynamics of each thymocyte population which is induced by the administration of a glucocorticoid, i.e. dexamethasone. The proposed approach relies on extending a four compartment thymus model based on differential equations by adding perturbation terms either globally (at the level of each equation) or locally (at the level of proliferation, death, and transfer rates). By fitting the perturbed model with experimental data on mice thymi collected before and after the administration of dexamethasone, it was possible to estimate the relevant parameters using a population-based stochastic search method. The fitted model is further used to conduct a quantitative analysis on the differentiated impact of dexamethasone on each T-cell population and on proliferation, death, and transfer processes. The obtained quantitative information on the perturbation could be used to explore and modify the flow of thymocytes between thymus compartments in order to elucidate the mechanisms of thymus involution and its subsequent regeneration. Since glucocorticoids are raised in many pathological situations, such a model could be useful in evaluating the impact of diseases on thymocyte dynamics in the thymus. © 2014 Elsevier Ltd.

Costescu C.I.,Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania Timisoara | David I.,Banats University of Agricultural science and Veterinary Medicine King Michael I of Romania Timisoara | Gruia A.T.,Regional Center for Immunology and Transplant
Beilstein Journal of Organic Chemistry | Year: 2014

Ocimum basilicum L. essential oil and its β-cyclodextrin (β-CD) complex have been investigated with respect to their stability against the degradative action of air/oxygen and temperature. This supramolecular system was obtained by a crystallization method in order to achieve the equilibrium of complexed-uncomplexed volatile compounds in an ethanol/water solution at 50 ° C. Both the raw essential oil and its β-CD complex have been subjected to thermal and oxidative degradation conditions in order to evaluate the protective capacity of β-CD. The relative concentration of the O. basilicum L. essential oil compounds, as determined by GC-MS, varies accordingly with their sensitivity to the thermal and/or oxidative degradation conditions imposed. Furthermore, the relative concentration of the volatile O. basilicum L. compounds found in the β-CD complex is quite different in comparison with the raw material. An increase of the relative concentration of linalool oxide from 0.3% to 1.1%, in addition to many sesquiterpene oxides, has been observed. β-CD complexation of the O. basilicum essential oil modifies the relative concentration of the encapsulated volatile compounds. Thus, linalool was better encapsulated in β-CD, while methylchavicol (estragole) was encapsulated in β-CD at a concentration close to that of the raw essential oil. Higher relative concentrations from the degradation of the oxygenated compounds such as linalool oxide and aromadendren oxide were determined in the raw O. basilicum L. essential oil in comparison with the corresponding β-CD complex. For the first time, the protective capability of natural β-CD for labile basil essential oil compounds has been demonstrated. © 2014 Hədərugəet al; licensee Beilstein-Institut.

Hadaruga D.I.,Polytechnic University of Timisoara | Unlusayin M.,Akdeniz University | Gruia A.T.,Regional Center for Immunology and Transplant | Birau C.,Banats University Of Agricultural Science | And 2 more authors.
Beilstein Journal of Organic Chemistry | Year: 2016

The thermal and oxidative stability of Atlantic salmon oil (Salmo salar L.) as well as its β-cyclodextrin (β-CD) complexation ability has been verified for the first time. The main omega-3 fatty acids, EPA and DHA, were significantly degraded, even at 50 °C. Their relative concentrations decrease from 6.1% for EPA and 4.1% for DHA to 1.7% and 1.5% after degradation at 150 °C, respectively. On the other hand, the relative concentrations of monounsaturated and saturated fatty acids remained constant or slightly increased by a few percent after degradation (e.g., from 10.7% to 12.9% for palmitic acid). Co-crystallization of ASO with β-CD at a host-guest ratio of 1:1 and 3:1 from an ethanol-water mixture and kneading methods has been used for the preparation of β-CD/ASO complexes. The analysis of the complexes by thermogravimetry, differential scanning calorimetry (DSC), and Karl Fischer titration (KFT) as well as the decrease of the "strongly-retained" water content confirm the formation of the inclusion compound. Furthermore, the DSC parameters correlate well with the KFT kinetic data for β-CD/ASO complexes. © 2016 Hǎdǎrugǎ et al; licensee Beilstein-Institut. License and terms: see end of document.

Mic A.A.,Victor Babes University of Medicine and Pharmacy Timisoara | Gruia A.T.,Regional Center for Immunology and Transplant | Suciu M.,Victor Babes University of Medicine and Pharmacy Timisoara | Azghadi S.M.R.,Victor Babes University of Medicine and Pharmacy Timisoara | Mic F.A.,Victor Babes University of Medicine and Pharmacy Timisoara
Annals of the Romanian Society for Cell Biology | Year: 2015

We investigated the changes in the thymocyte subpopulations of the involuting thymus in the experimental diabetes and propose a molecular mechanism for thymus involution in diabetes. Mice were injected with streptozotocin and thymus weight, body weight, and glycemia were measured at various time points. The dynamics of thymocyte populations, their apoptosis and cell cycle during diabetes were analyzed by flow-cytometry and in vivo 5-ethynyl-2´-deoxyuridine labeling. Concentration of corticosterone in thymus and serum was assessed by ELISA. In hyperglycemic animals the thymus gradually involutes, losing much of the thymocytes by apoptosis while the S-phase dividing thymocytes are significantly reduced. The thymocyte composition changes, the percentage of CD4+CD8+ double-positive thymocytes droped while the CD4-CD8-double-negative and the mature thymocytes increaseed. The chemical stress caused by hyperglycemia leads to elevation of glucocorticoids which could induce thymocyte apoptosis and blockage of their proliferation. Thymus involutes rapidly after the onset of hyperglycemia due to elevated apoptosis in thymocytes and reduced proliferation of thymocytes. The double-positive thymocytes decreased while the double-negative and mature ones increased. Elevated serum levels of glucocorticoids seem to cause both thymocytes apoptosis and arrest of proliferation. The components of the inflammatory reaction (glucocorticoids), rather than hyperglycemia, damage the organ. © The Romanian Society for Cell Biology.

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