Regional Center for Food and Feed

Alexandria, Egypt

Regional Center for Food and Feed

Alexandria, Egypt
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Ismail A.F.M.,National Center for Radiation Research And Technology | Salem A.A.M.,Regional Center for Food and Feed | Eassawy M.M.T.,Regional Center for Food and Feed
Journal of Photochemistry and Photobiology B: Biology | Year: 2016

The activity of flaxseed oil (FSO) on gamma-irradiation (7 Gy) and/or carbon tetrachloride (CCl4) induced acute neurotoxicity in rats' brain was investigated. The results revealed a significant decrease (p < 0.05) in superoxide dismutase (SOD), catalase (CAT), glutathione-peroxidase (GSH-Px) activities, reduced glutathione (GSH) and manganese (Mn) contents. Further, a significant elevation (p < 0.05) in malondialdehyde, nitric oxide (NO), Tumor Necrosis Factor-alpha (TNF-α), Interleukin-1-beta (IL-1β), Interleukin-6 (IL-6), transforming growth factor-beta-1 (TGF-β1), iron (Fe), calcium (Ca), copper (Cu) and magnesium (Mg) levels were observed. Furthermore, the relative ratio of xanthine oxidase (XO) and inducible nitric-oxide synthase (iNOS) gene expression levels were elevated in the brain tissues of γ-irradiated and CCl4 intoxicated animals. Those effects were augmented due to the effect of CCl4-induced toxicity in γ-irradiated rats. The treatment of FSO displayed significant amendment of the studied parameters in the brain tissues of γ-irradiated and CCl4 intoxicated animals. FSO has a neuroprotective effect against CCl4-induced brain injury in gamma-irradiated rats. This effect is interrelated to the ability of FSO to scavenges the free radicals, enhances the antioxidant enzymes activity, increases GSH contents, down-regulates the inflammatory responses, ameliorates the iron, calcium, copper, magnesium, manganese levels and inhibiting the gene expression level of XO and iNOS in the brain tissues of intoxicated animals. In conclusion, this study demonstrated that the potent antioxidant and anti-inflammatory activities of FSO have the ability to improve the antioxidant status, suppress the inflammatory responses, and regulate the trace elements in the brain tissues of γ-irradiated, CCl4, and their combined effect in intoxicated animals. Consequently, FSO exhibited neuroprotective activity on γ-irradiated, CCl4, and their combined effect induced brain injury in rats. © 2016 Elsevier B.V. All rights reserved.


Elsanhoty R.M.,Menoufia University | Ramadan M.F.,Zagazig University | Ramadan M.F.,University of Umm Al - Qura | El-Gohery S.S.,University of Dammam | And 2 more authors.
Food Control | Year: 2013

Aflatoxins (AFs), the secondary metabolites produced by species of Aspergillus, have harmful effects on humans, animals, and crops that result in illnesses and economic losses. This investigation was designed to assess the potential of four strains of lactic acid bacteria (LAB) for removing AFs invitro. The stability of AFs complexes formed with LAB in viable and non-viable (heat treated) forms was assessed. The strain with the highest ability to bind AFs was selected to study its impact on removing AFs from contaminated wheat flour during baladi bread baking process. Three treatments of baladi bread produced from contaminated wheatflour were formulated including treatment (A) as control fermented by bakery yeast, treatment (B)fermented by Lactobacillus rhamnosus TISTR 541 and treatment (C) fermented by the mixture of bakery yeast and L. rhamnosus. The samples were collected during different steps of bread baking process. The results indicated that there were significant differences between the amounts of AFB and AFG detected in each sample and there were significant differences between the strains in their ability to bind AFB and AFG in the viable and heated stage. L. rhamnosus was the highest strain able to bind the AFB1 and treatment C was the highest in removing of AFs from contaminated wheat flour during baking process. © 2013 Elsevier Ltd.


Gibriel Y.A.Y.,King Abdulaziz University | Hamza A.S.,Regional Center for Food and Feed | Gibriel A.Y.,Ain Shams University | Mohsen S.M.,Cairo University
Journal of Food Safety | Year: 2011

The effect of mint (Mentha viridis) essential oil (EO) on Aspergillus flavus growth and aflatoxin production was investigated in stored corn and evaluated. A. flavus was isolated from stored corn and identified as an aflatoxigenic strain. Conical flasks of 250mL each containing 100g of corn were sterilized by autoclave (121C, 15lbs/in 2 for 15min) and artificially inoculated with A. flavus approximately 10 6cfu/mL (control sample). Other samples were inoculated in the same manner as the control, but with the addition of different values of mint EO (50, 100, 200, 300, 500 and 700μL/100g corn). Mint EO was fungistatic at all concentrations, fungicidal and antiaflatoxigenic at 300, 500 and 700μL/100g corn from 7 days storage up to the end of storage at 21 days. Minimum inhibitory concentration for A. flavus was found to be 200μL/100g corn, and also limited the aflatoxin production to be under the safety level of 20ppb determined by the Food and Agricultural Organization and World Health Organization. Mint EO could be used as fungicidal and antiaflatoxigenic agents for A. flavus in stored corn especially in granaries. Mint EO is safe, acceptable and extends the storage period of corn. PRACTICAL APPLICATION: It could be applied to preserve cereals, especially in granaries for a long storage period, that mint essential oil Mentha viridis can be used as antifungal and antiaflatoxigenic and a safe alternative instead of chemicals, which have hazardous effects on human and animal health. © 2011 Wiley Periodicals, Inc.


Khalil N.S.A.M.,Regional Center for Food and Feed
International Journal of Applied Chemistry | Year: 2011

Novel synthesis of 1,ω-bis[4-(4-amino/and or arylideneamino-5-thioxo- 1H- 1,2,4-triazol-3yl) phenoxy]alkanes 4-6/and or 7-30 as well as 1,ω-bis[4-(6- aryl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazol-3-yl)phenoxy] alkanes 31-48 were achieved starting with 1,ω-bis[4-(hydrazinecarbonyl) phenoxy]alkanes 1-3. Some of the newly synthesized compounds were evaluated for their in vitro antimicrobial activity against different strains of Gram-positive bacteria, Gram-negative bacteria, yeast and fungi. The screening results revealed that most of the tested compounds exhibited broad spectrum inhibitory effects against different test microbes. © Research India Publications.


A series of novel 1,ω-bis[4-[(arylcarbamothioylamino)carbamoyl] phenoxy]- alkanes as well as 1,ω-bis[4-(4-aryl-5-thioxo-1H-1,2,4-triazol- 3-yl)phenoxy]- alkanes were synthesized and their structures were assigned based on analytical and spectral data. Of these compounds, some were selected and evaluated for their in vitro antimicrobial activity against different strains of Gram-positive bacteria, Gram-negative bacteria, yeast and fungi. The screening results revealed that most of the tested compounds exhibited broad spectrum inhibitory effect against the test organisms. © Research India Publications.


Versatile simple efficient routes with good to excellent yields towards different functionalized 1,ω-bis(1,2,4-triazoles), 21-28 membered 1,2,4-triazole fused macrocyclic and/or lariat macrocyclic oxaazathia crown Schiff bases and/or amines have been investigated. Antimicrobial screening of some selected compounds revealed different inhibitory effects against Aspergillus fumigatus RCMB 002008 (1), Penicillium italicum RCMB 001018 (1), Syncephalastrum racemosum RCMB 016001 Candida albicans RCMB 005003, Staphylococcus aureus RCMB 106-001 (1), Pseudomonas aeruginosa RCMB 102-002, Bacillus subtilis RCMB 101-001 and Escherichia coli RCMB 103-001. © 2010 Elsevier Masson SAS. All rights reserved.


Salah T.A.,Regional Center for Food and Feed | Mohammad A.M.,Cairo University | Mohammad A.M.,The British University in Egypt | Hassan M.A.,Regional Center for Food and Feed | El-Anadouli B.E.,Cairo University
Journal of the Taiwan Institute of Chemical Engineers | Year: 2014

Hydroxyapatite nanorods (nHAp) and nano-hydroxyapatite chitosan composites (nHApCs) were proposed for the removal of Cd2+ ions in water treatment. The high resolution transmission electron microscopy, energy dispersive X-ray analysis, X-ray diffraction spectrophotometer, Fourier transform infrared spectrophotometer and Zeta potential measurements were all employed to reveal the morphology, composition, crystal structure, functionality and stability of the prepared sorbents (nHAp and nHApCs). The potential of these synthesized sorbents to remove Cd2+ ions from aqueous solutions was investigated in batch experiments, where several parameters such as the sorbate/sorbent's contact time, initial Cd2+ ions concentration, pH and sorbent dosage were investigated. The equilibrium concentration of Cd2+ ions was identified by the atomic absorption spectrophotometry. The Cd2+ uptake was quantitatively evaluated using the Pseudo second order kinetic equation, Freundlich and Langmuir models. It is remarkable that with these sorbents (nHAp, nHApCs), up to 92% of Cd2+ could be removed "100ppm initial cadmium concentration in 200mL, 0.4g nHAp and pH=5.6″. The sorption capacity of nHAp and nHApCs to Cd2+ was 92 and 122mg/g respectively, which appears excellent when compared to other previously reported materials. This capacity could be enhanced by increasing initial Cd2+ concentration and the nHAp/Cd2+ mass ratio. Furthermore, the sorbents' regeneration was addressed and found promising. © 2013 Taiwan Institute of Chemical Engineers.


El-Chaghaby G.A.,Regional Center for Food and Feed | Ahmad A.F.,Regional Center for Food and Feed | Ramis E.S.,Regional Center for Food and Feed
Arabian Journal of Chemistry | Year: 2014

The present work was conducted aiming to evaluate the effect of different solvent extracts on the antioxidant and antibacterial activities of Annona squamosa L. leaves. Four solvents were chosen for the study namely; methanol 80%, acetone 50%, ethanol 50% and boiling water. Acetone and boiling water gave the highest extraction yields as compared to methanol and ethanol. Total phenolic contents of the four extracts were significantly different with acetone being the most efficient solvent and water being the least efficient one. Correlation coefficient between the total antioxidant and total phenolic content was found to be R2=0.89 suggesting the contribution of phenolic compounds of the extract by 89% to its total antioxidant activity. The extracts were capable of scavenging H2O2 in a range of 43-54%. Reducing power of the extracts increased by increasing their concentration. The extracts were found to exert low to moderate antibacterial activity compared to a standard antibacterial agent. The bacterial inhibition of the extracts was found to positively correlate with their phenolic contents. © 2011.


Badr S.E.A.,Regional Center for Food and Feed | Sakr D.M.,Regional Center for Food and Feed | Mahfouz S.A.,Regional Center for Food and Feed | Abdelfattah M.S.,Helwan University
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2013

This study was conducted to investigate the chemical composition and biological impacts of licorice drink in both infusion and tea forms. The chemical composition was determined by quantifying protein, fats, moisture, ash, fiber, carbohydrates and minerals. The most abundant minerals detected in licorice in both tea and infusion forms were calcium, sodium, phosphorus, potassium and iron, respectively. Zinc and copper were detected as trace elements. Essential and non essential amino acids were present in all licorice forms. HPLC analysis of the organic acids in licorice forms showed the presence of several aliphatic acids such as butyric acids, tartaric acid and acetic acids. The methanolic extract of licorice showed cytotoxic activities against intestinal carcinoma cell line (Caco-2) and prostate carcinoma cell line (PC-3) with IC50 values of 40 and 40.6 μg/ml, respectively. The effects of licorice in infusion and tea forms on body weight gain, white and red blood cells, hemoglobin and blood platelets, liver enzymes, kidney functions, total cholesterol and triglyceride levels, and some serum mineral levels were studied in vivo.


El-Chaghaby G.A.,Regional Center for Food and Feed | Ahmad A.F.,Regional Center for Food and Feed
Oriental Journal of Chemistry | Year: 2011

The biosynthesis of silver nanoparticles was successfully achieved using Pistacia lentiscus leaves extract and silver nitrate. The plant extract was mixed with silver nitrate, incubated at 5O0C and synthesis of nanoparticles was followed using UV-Vis spectroscopy. The reaction was completed in three hours and the resultant nanoparticles were characterized using XRD and TEM techniques The average size of the produced nanoparticles was found to 24-26 nm. The antifungal and antibacterial activities of the biosynthesized silver nanoparticles were investigated using disc diffusion method. The results showed that these nanoparticles displayed antimicrobial a good activity against fungal as well as bacterial cultures.

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