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Orbassano, Italy

Borrione P.,Foro Italico University of Rome | Spaccamiglio A.,Regional Anti doping Center | Rizzo M.,Foro Italico University of Rome | Termine A.,University of Turin | And 5 more authors.
Journal of Biological Regulators and Homeostatic Agents | Year: 2011

The use of iron supplements should be a judicious choice, primarily when considering the possible risks deriving from an unjustified treatment. In trained athletes, levels of ferritin between 15 and 30 μg/L are frequently observed. Within this ferritin range, the usefulness of iron supplementation is still controversial. The aim of the present study is to evaluate the clinical usefulness of hepcidin assessment in the analysis of the iron status of young non-anemic athletes. Fifty young athletes were enrolled. The subjects were divided into 4 groups according to their ferritin levels. No statistically significant difference was found regarding hepcidin levels between athletes with ferritin lower than 15 μg/L and those in the 15-30 μg/L range. Similarly, no difference was found between athletes with ferritin higher than 50 μg/L and those in the 30-50 μg/L range. On the contrary, statistically significant differences were found between athletes with ferritin levels ranging from 15 to 30 μg/L and those in the 30-50 μg/L range. The present study suggests that serum ferritin levels below 30 μg/L indicate an asymptomatic iron deficiency status inhibiting hepcidin expression and that 30 μg/L should be considered the ferritin cut-off when considering an iron supplementation in young athletes. Copyright © by BIOLIFE, s.a.s. Source

Borrione P.,Foro Italico University of Rome | Marchiaro G.,Regional Anti doping Center | Vincenti M.,Regional Anti doping Center | Sciacca A.,Regional Anti doping Center | And 4 more authors.
Journal of Sports Medicine and Physical Fitness | Year: 2011

Aim. Even if youths are generally perceived to be healthy, adolescent years are associated with significant morbidity. Screening and counselling programmes seem to be cost-effective but adolescents prefer to rely on health care services for the treatment of diagnosed diseases or injuries rather than for preventive actions. Age oriented studies are needed for better understanding the health needs of adolescents in order to provide an adequate offer of preventive opportunities. Methods. Eight hundred youths ranging from 13 to 18 years of age were recruited. Health status and risks were clustered into the following five categories: clinical assessment, substance use/abuse, nutritional habits, alcohol and tobacco consumption, physical status. Surprisingly, 33% of the youths were suggested to perform further clinical assessment and even more interestingly a significant number of them received a diagnosis of a symptomatic disorder for which he or she did not previously consider a medical visit to be necessary. Results. As expected, alcohol consumption, tobacco smoking, drug use/abuse and sedentary habit represent the risky lifestyles commonly followed by adolescents. Conclusion. The present study confirms the importance of screening programs addressed to health issues and behav-ioural attitudes of adolescents even in light of the fact that they may underestimate even indicative symptoms. Source

Dovio A.,University of Turin | Roveda E.,University of Milan | Sciolla C.,University of Turin | Sciolla C.,Regional Anti doping Center | And 10 more authors.
European Journal of Applied Physiology | Year: 2010

Intense physical exercise activates the hypothalamic-pituitary- adrenocortical axis but little is known about changes in glucocorticoid sensitivity at the target cell level. No data are available on the acute effects of exercise on 11β-hydroxysteroid dehydrogenase (11β-HSD) type 1 activity, which generates biologically active cortisol from inactive cortisone and is expressed also in skeletal muscle. Fifteen healthy, trained males (age mean ± SE 28 ± 1) were assessed on three non-consecutive days: at rest, during an endurance and strength sessions. During each session, between 1000 and 1600 hours, 6-h urine and four salivary samples were collected. Urinary total tetrahydrocortisol (THF) + alloTHF, tetrahydrocortisone (THE), cortisol (F) and cortisone (E) were measured with HPLC-tandem mass spectrometry; urinary-unconjugated F and E were measured by HPLC-UV. Salivary cortisol and interleukin (IL)-6 were measured by RIA and ELISA, respectively. Both endurance and strength exercises caused an increase in (THF + alloTHF)/THE ratio (mean ± SE 1.90 ± 0.07 and 1.82 ± 0.05 vs. 1.63 ± 0.06, P < 0.01 and P = 0.03, respectively), consistent with increased systemic 11β-HSD type 1 activity. No relationship was found with age, BMI, VO 2max maximal power load or perceived exertion. No significant change was apparent in F/E ratio, an index of 11β-HSD type 2 activity. No effect of exercise on salivary cortisol and IL-6 was observed, whereas a significant effect of sampling time was found. Intense physical exercise acutely increases systemic 11β-HSD type 1 activity in humans. Such an increase may lead to higher cortisol concentration in target tissues, notably in skeletal muscle where it could contribute to limit exercise-induced muscle inflammatory response. © 2009 Springer-Verlag. Source

Borrione P.,Regional Anti doping Center | Borrione P.,University of Turin | Spaccamiglio A.,Regional Anti doping Center | Parisi A.,University Institute of Movement Science | And 5 more authors.
International Journal of Laboratory Hematology | Year: 2010

Automated haematological analysers still represent the gold standard for the study of reticulocyte maturation even if this technique is based on structural properties and staining affinity rather than on functional aspects. On the contrary, flow cytometry allows the simultaneous analysis of multiple cellular characteristics including functional features. Aim was to investigate whether simultaneous analysis ofdifferent reticulocyte parameters using flow cytometry may add functional information when considering their pattern of maturation. Thirty-nine healthy donors (H) and 31 haemodialysed patients on treatment with rHuEpo (HDT) were analysed. Reticulocyte counts and their stages of maturation were studied both with ADVIA 2120 and by flow cytometry. TO/CD71 scattergraph reticulocyte analysis designed a peculiar distribution which was similar among the same group of subjects (H or HDT), but different between H and HDT. distribution of the percentage of reticulocytes in low, medium and high boxes calculated by ADVIA 2120 did not show any difference between H and HDT groups, while the analysis using flow cytometry pointed out statistically significant differences between H and HDT groups in the three boxes where the TO+/CD71+ reticulocytes were localized. The present study suggests that TO/CD71 analysis was reproducible and could detect different pattern of maturation of a particular clinical setting. Source

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