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Akhtar L.H.,Regional Agricultural Research Institute | Pervez M.A.,University of Agriculture at Faisalabad | Nasim M.,Soil and Water Testing Laboratory
Pakistan Journal of Agricultural Sciences | Year: 2011

Genetic variability, heritability and interrelationships for seed yield and its components (days taken to 50% flowering, plant height, number of pods per plant, days taken to 90% maturity, 100-seed weight) were estimated in 20 advance genotypes of chickpea collected from various sources along with one check variety (Pb-2000). Highly significant differences existed among the genotypes tested for all the traits. Genotype BRC-61 recorded highest seed yield of 2396 kg ha-1 where as check variety Bunjab-2000 yielded only 2068 kg ha-1. Genotype BRC-61 was the earliest in maturity and had highest weight of 100 seeds. Significant and positive correlations were found between yield and 100-seed weight, number of pods per plant and plant height. Broad sense heritability ranged from 89.61 (seed yield) to 99.99% (100-seed weight). Heritabilities for 100-seed weight and number of pods per plant were the greatest compared to other traits. Phenotypic coefficient of variation (PCV) for days taken to flowering, days taken to maturity, plant height and seed yield were higher than genotypic coefficient of variations (GCV) which means that the expression of these traits is more influenced by environmental effects. It is, therefore, suggested that the grain yield could be improved by using the 100-seed weight and number of pods per plant as selection criterion in chickpea. Source

Mukhtar T.,Pmas Arid Agriculture University | Hussain M.A.,Regional Agricultural Research Institute | Kayani M.Z.,Green Belt Project | Aslam M.N.,Islamia University of Bahawalpur
Crop Protection | Year: 2014

The root-knot nematode, Meloidogyne incognita, is one of the major limiting factors affecting plant growth and yield causing an estimated $100 billion loss per year worldwide. Synthetic pesticides, though instantaneously effective, are usually prohibitively expensive, not readily available, may cause hazards to both man and livestock, and inflict injury to the environment. Notable among the alternatives to nematicides is the use of resistant cultivars which are inexpensive and eco-friendly. In the present studies, twelve okra ( Abelmoschus esculentus L) cultivars were evaluated for their resistance against M.incognita under field conditions. Ten-day old okra plants of test cultivars were inoculated with 3000 freshly hatched second stage juveniles of M.incognita. The nematode caused reductions in various growth parameters of all the cultivars to varying levels over their respective controls. None of the cultivars was found completely resistant. The cultivar 'Sharmeeli' was highly susceptible as >100 galls were recorded on the roots. Sharmeeli also showed maximum reductions in growth among the cultivars evaluated. The cultivars Anmol and Sindha were susceptible with 71-100 galls. The cultivars Sabz Pari, Super Star, PMS-55 and PMS Beauty were moderately susceptible with 31-70 galls and comparatively less reductions in growth. Cultivars Sanam, Dikshah, Arka Anamika, Ikra-1 and Ikra-2 with 11-30 galls were rated as moderately resistant and showed less damage by the nematode as compared to susceptible cultivars and their planting could provide a useful tool to control root-knot nematodes. © 2013 Elsevier Ltd. Source

Kayani M.Z.,Green Belt Project | Mukhtar T.,Pmas Arid Agriculture University | Hussain M.A.,Regional Agricultural Research Institute
Crop Protection | Year: 2012

In view of the recently increased interest in developing plant origin nematicides as a surrogate to chemical nematicides, the present study was carried out to assess the nematicidal potential of two antagonistic plants Cannabis sativa L. (Cannabaceae) and Zanthoxylum alatum Roxb. (Rutaceae) against the most devastating root-knot nematode, Meloidogyne incognita responsible for colossal yield losses in cucumber. The leaves of C. sativa and Z. alatum were incorporated in the soil at the rate of 0, 2, 4, 6, 8, 10 and 20 g per kg of soil. After decomposition, cucumber (cv. Royal Sluis) seeds were sown and inoculated with 2000 s stage juveniles of M. incognita ten days after emergence. Data on growth variables and nematode infestations were recorded after six weeks of inoculation. Both the plants significantly reduced nematode infestations and enhanced plant growth criteria compared to the untreated check. The reductions in number of galls, egg masses, nematode fecundity and build up caused by C. sativa were significantly higher as compared to Z. alatum. Maximum reductions in these variables were recorded with 20 g dosage. The addition of C. sativa and Z. alatum to the soil as organic amendment can work very well as nematicides and can be successfully used for controlling root-knot nematodes replacing traditional chemical treatments and avoiding environmental pollution. © 2012 Elsevier Ltd. Source

Hussain M.A.,Regional Agricultural Research Institute | Mukhtar T.,Pmas Arid Agriculture University | Kayani M.Z.,Green Belt Project | Aslam M.N.,Islamia University of Bahawalpur | Ul-Haque M.I.,Pmas Arid Agriculture University
Pakistan Journal of Botany | Year: 2012

Okra (Abelmoschus esculentus (L.) Moench) is one of the important vegetables of the tropical and subtropical areas. In Pakistan okra is cultivated on thousands of hectares but the yield obtained is relatively lower, for which there are many constraints including prevalence of diseases caused by different pathogens. Among various pathogens responsible for the low yield, the root-knot disease incited by Meloidogyne spp., is of considerable economic importance and cause annual losses in tropics to an extent of 22 per cent. As there is meager information regarding the association of root-knot nematodes with okra in the country, the present studies were conducted in 17 districts of the Punjab province of the country to determine the occurrence, prevalence and intensity of root-knot nematodes in okra cultivations in the vegetable growing areas of Punjab. The survey revealed that root-knot nematodes prevailed in 85.28% of okra fields with an average incidence of 38.89%. Hundred per cent prevalence was recorded in Multan, Okara, Dera Ghazi Khan, Bahawalnagar, Vehari, Rahim Yar Khan and Rawalpindi districts and a minimum prevalence of 22.4% was found in Lodhran district. The incidence was above 60% in Bahawalnagar, Rahim Yar Khan, Dera Ghazi Khan and Vehari and was only 4.44% in Lodhran. The severity of infection of the nematodes was highest in Bahawalnagar and Vehari, while it was the lowest in Lodhran. Of the four most common root-knot species, M. incognita constituted 74.74%, M. javanica 24.02%, M. arenaria 1.57% and M. hapla 0.78%. Source

Mukhtar T.,Pmas Arid Agriculture University | Kayani M.Z.,Green Belt Project | Hussain M.A.,Regional Agricultural Research Institute
Industrial Crops and Products | Year: 2013

Because of being costly and pernicious to the environment and human health, the use of nematicides has become prohibitive in many countries and the management of plant parasitic nematodes using antagonistic plants can be a very attractive alternative. In the present studies the effectiveness of aqueous extracts of Cannabis sativa and Zanthoxylum alatum was assessed on hatching, mortality and infectivity of Meloidogyne incognita at different concentrations viz. S, S:1, S:5, S:10, S:25, S:50 and S:100. Both the plants had significant effects on juvenile mortality and hatching inhibition in a dose-dependent manner. Mortality and hatching inhibition caused by C. sativa were significantly higher than that of Z. alatum. Time duration also affected mortality and hatching inhibition significantly. Significant inhibition in invasion of M. incognita juveniles on cucumber cv. Royal Sluis was observed by different treatments with extracts. M. incognita juveniles exposed to 'S' extracts of C. sativa and Z. alatum for 24 and 48. h caused no infection. Exposure for 12 and 6. h caused more than 95 and 90% reductions in infectivity of M. incognita juveniles respectively. Similarly, soil drench and root dip treatments also caused significant reductions in infection. Reduction in infectivity was found to be significantly higher with extracts of C. sativa as compared to Z. alatum and decreased in a dose-responsive manner. The results of the studies showed that the extracts of test plants, commonly found locally, possess high potentials for the control of root-knot nematodes and could be the possible replacement for synthetic nematicides. © 2012 Elsevier B.V. Source

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