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Seoul, South Korea

Park S.H.,Konkuk University | Yoon J.,Seoul National University of Science and Technology | Park J.H.,Konkuk University | Kim B.Y.,Konkuk University | And 7 more authors.
Tissue Engineering and Regenerative Medicine | Year: 2015

This article overall describes the development of histological method especially, cartilage and bone research in regenerative medicine. Kawamoto’s film method, which has been recently introduced into our laboratory, has been applied in various fields that make sectioning of hard tissues easier than the conventional method. Moreover, this method also does not require the time-consuming chemical fixation and/or decalcification process. Kawamoto method involves the use of the adhesive plastic film instead of a cover glass where the thin tissue sections are attached efficiently at low temperatures (-25°C). Furthermore, the histological method preserves the enzymatic activity in the fresh sections in comparison to that of chemically-treated tissue sections. In fact, we used this Kawamoto’s film method in one of our researches in which drug repositioning was employed for cartilage regeneration. Some of the Kawamoto-processed tissue sections are featured in this review article. Therefore, the application of this tissue preparation technique allowed effective and histological and histochemical studies within a shorter preparation time with ease and convenience. In the future, this Kawamoto method may offer further applications in the preparation of a more diverse tissues and samples not only in the preclinical but also in the clinical studies. © 2015, The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht.

Lee S.,Konkuk University | Jang I.J.,Konkuk University | Yang W.,Konkuk University | Koo M.,Konkuk University | And 5 more authors.
Tissue Engineering and Regenerative Medicine | Year: 2015

In the development of novel tissue engineering and regenerative medicine techniques in humans, preclinical studies using laboratory animal models are required to examine the pathogenic mechanisms and the potential treatment of diseases for the clinics and an indispensable step for determination of their safety and efficacy. Regrettably, the etiological pathology of diseases of spontaneous diseases may not be exactly the same despite the similarities in signs and symptoms observed in artificial laboratory animal models. Therefore, the artificial laboratory animal model is not fully suitable for studies on specific target diseases. However, the animals, especially companion animal, that have spontaneous and frequently occurring diseases due to genetic and environmental factors mostly likely mimic the naïve pathology of diseases in humans. Consequently, these species can be better subjects of preclinical studies and veterinary clinical trials in order to possibly gauge the effectiveness of the therapeutic strategies in the clinical stage. Hence, research on the spontaneous and frequently diagnosed diseases of animals predisposed by the type of breeding, age, and gender that are applicable in tissue engineering and regenerative medicine could be an important data for future advancements. In this study, we conducted a survey on the spontaneous and common diseases of canine and feline species to provide a foundation on the effectiveness and practical application of clinical studies. Therefore, this review can contribute significant development in tissue engineering and regenerative medicine in the future. © 2015, The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht.

Lee K.M.,Konkuk University | Han Y.,Konkuk University | Yoon J.,Seoul National University of Science and Technology | Lee S.,Konkuk University | And 2 more authors.
Tissue Engineering and Regenerative Medicine | Year: 2015

The trend of regenerative medicine has been gaining a rising global attention and has been the target of growing research funds. This includes the importance of the application of cell-based therapy, especially stem cells, for various diseases. Hence, regulations on the use of cells and the pros and cons of its application in the clinical level should also be given equal attention. Recently, regulations in Japan have been reviewed and revised to create more effective and competitive marketing strategy. The Regenerative Medicine Promotion Law was established to provide prompt and safe regenerative medicine products. The Acts on the Safety of Regenerative Medicine entails that medical technologies can be categorized into 3 classes of regenerative medicines based on their risks potentials. It also determines the proper regulation or requirement for each class. Amendment of Pharmaceutical Affairs Act, on the other hand, is regulation in which products can be approved with temporary conditions to enable prompt provision of the products to patients after the safety and efficacy is confirmed. Due to these mitigations in Japan in the advancement of regenerative medicines, we can predict that Japan can be one of the top nations in the production and marketing stem cell therapeutic products by attracting Japanese as well as global companies. Hence, monitoring the changes in the legal policies of regenerative medicine in Japan could be advantageous in the advancement of the current provisions in South Korea geared towards the better achievements in providing the human population with safe, prompt, and effective regenerative medicine therapeutic strategies and products. © 2015, The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht.

Yang W.,Konkuk University | Lee S.,Konkuk University | Yoon J.,Seoul National University of Science and Technology | Lee J.I.,Konkuk University | Lee J.I.,Regeniks Co.
Tissue Engineering and Regenerative Medicine | Year: 2015

The stem cell therapy in veterinary medicine continues to grow both experimentally and clinically. Application of adipose-derived mesenchymal stem cell (MSC), umbilical cord blood-derived MSC, bone marrow-derived MSC and skeletal muscle-derived MSC for treatment of various diseases are currently in use for several species. However, the differential efficacies of various approaches are still being investigated. In stem cell therapy, common disease animal models include mouse, rat, rabbit, swine, canine, caprine, equine and dolphin are used. Whereas for clinical models, canine, equine are dolphin are used. We need attention and participation from the researchers of tissue engineering and regenerative medicine, and now it is necessary to develop mutual understanding and cooperation between veterinary and human medicine fields. This review is focused on some of the recent status of stem cell therapy in the veterinary medicine. © 2015, The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht.

Balolong E.,Konkuk University | Lee S.,Konkuk University | Lee S.,Regeniks Co. | Nemeno J.G.,Konkuk University | Lee J.I.,Konkuk University
Stem Cells International | Year: 2016

There is an increasing concern that the term adipose tissue-derived stem cell (ASC) is inappropriately used to refer to the adipose stromal vascular fraction (SVF). To evaluate the accuracy and quality of reporting, 116 manuscripts on the application of ASC in humans and animals were examined based on the 2013 published International Federation for Adipose Therapeutics and Science (IFATS)/ International Society for Cellular Therapy (ISCT) joint statement and in reference to current guidelines for clinical trials and preclinical studies. It is disconcerting that 4 among the 47 papers or 8.51% (CI 2.37-20.38) surveyed after publication of IFATS/ISCT statement reported using ASCs but in fact they used unexpanded cells. 28/47 or 59.57% (CI 44.27-73.63) explicitly reported that adherent cells were used, 35/47 or 74.47% (CI 59.65-86.06) identified expression of surface markers, and 25/47 or 53.19% (CI 14.72-30.65) verified the multilineage potential of the cells. While there are a number of papers examined in this survey that were not able to provide adequate information on the characteristics of ASCs used with some erroneously referring to the SVF as stem cells, there are more room for improvement in the quality of reporting in the application of ASCs in humans and animals. © 2016 Ernesto Balolong et al.

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