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San Clemente, CA, United States

Mandal A.,Reliance Life science Pvt. Ltd | Bhowmik S.,Reliance Life science Pvt. Ltd | Patki A.,REGENESIS | Viswanathan C.,Reliance Life science Pvt. Ltd | Majumdar A.S.,Reliance Life science Pvt. Ltd
Stem Cell Research | Year: 2010

Human embryonic stem cells (hESCs) offer new avenues for studying human development and disease progression in addition to their tremendous potential toward development of cell-replacement therapies for various cellular disorders. We have earlier reported the derivation and characterization of Relicell® hES1, the first fully characterized hESC line generated from the Indian subcontinent. Recent studies have demonstrated discrete differences among hESC lines, in terms of both their growth properties and their differentiation propensity. To address some of these issues in the context of hESC research in India, we have recently generated two new hESC lines: Relicell® hES2 and Relicell®hES3. Both these cell lines were derived using a combinatorial approach of immunosurgery followed by mechanical surgery for inner cell mass isolation. The cell lines exhibit the usual hESC characteristics including their ability to differentiate both in vitro and in vivo to yield the three germinal layers. Whole genome microarray analysis of these cell lines was compared with Relicell®hES1 and it showed that approximately 9000 genes were expressed by these lines. As expected the expression pattern of these new cell lines bore close resemblance to that of Relicell®hES1. A majority of the pluripotency genes and the genes known to inhibit various differentiation pathways were also expressed by these cell lines. We also observed that each of these cell lines expressed a unique set of genes that are mutually exclusive from each other. These results represent the first detailed characterization of a set of hESC lines originating from India. © 2010 Elsevier B.V. Source


Birnstingl J.,REGENESIS
Pollution Engineering | Year: 2014

In-situ bioremediation of chlorinated compound, tetrachloroethene (PCE) dense non-aqueous phase liquid (DNAPL) using a range of controlled-release hydrogen substrates was used to enhance biodegradation. PCE was identified in a shallow, low concentration plume area and a deep, high concentration source area. Additionally, the presence of mudstone at depth presented an effective barrier against downward contaminant migration. HRC was used to maintain optimal redox conditions to allow reductive dechlorinating bacteria to grow without competition from non-productive methogenic microbes that would otherwise waste electron donor and produce methane, and characteristically lead to incomplete contaminant degradation. The three low-volume application substrates were mixed together before application and applied using a direct push injection technique on a grid pattern across the yard of the facility. In the shallow, permeable ground, the higher-volume application material, 3-D Microemulsion, was used to promote biological degradation of the contaminants. Source


Wilson S.,REGENESIS | Farone W.,Applied Power Concepts | Leonard G.,REGENESIS UK | Birnstingl J.,REGENESIS UK | Leombruni A.,REGENESIS Ltd.
Pollution Engineering | Year: 2013

Chemical oxidation has been a widely practiced remediation strategy to achieve rapid reductions of organic contaminants in groundwater, soils, and other environmental media. Persulfate, an oxidant used in environmental remediation, has historically been activated using the co-application of additional chemicals that are often costly to apply and can present a significant safety hazard on-site. PersulfOx, an all-in-one chemical product, promises to lower the cost and improve the safety of persulfate-based remediation projects by employing both alkaline activation and an advanced catalyst system. © 2013 REGENESIS Bioremediation Products. Source


Wilson S.,REGENESIS | Leonard G.,REGENESIS | Birnstingl J.,REGENESIS | Leombruni A.,REGENESIS | Farone W.,Applied Power Concepts
Pollution Engineering | Year: 2013

Chemical oxidation will continue to be a widely practiced remediation strategy to achieve rapid reductions of organic contaminants in groundwater, soils and other environmental media. Persulfate, an oxidant used in environmental remediation, has historically been activated using the co-application of additional chemicals that are often costly, labor intensive and can present a significant on-site safety hazard. A persulfate based, all-in-one chemical oxidant, promises to lower cost and improve the safety of remediation projects by employing alkaline activation and an advanced catalyst system. Source

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