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Marotta F.,ReGenera Research Group | Naito Y.,Immunology Research Institute and Clinic | Padrini F.,ReGenera Research Group | Xuewei X.,Peking University | And 7 more authors.
Journal of Biological Regulators and Homeostatic Agents | Year: 2011

There is increasing evidence that psychosocial stress can be viewed as a system-wide derangement of cellular homeostasis, with heightened oxidative stress and triggered proinflammatory mechanisms. The aim of this study is twofold: a) to replicate findings that psychological stress increases oxidative damage and b) to determine whether a fermented papaya preparation known to exert significant protective antioxidant properties could buffer such increases in nuclear DNA damage while also inducing epigenetic protective mechanisms. Twenty-eight sedentary men and women (age range: 28-52), who reported living a stressful lifestyle but with an overall positive attitude, were recruited for this study. Chronic diseases as well as severe burnout and use of drugs for anxiety constituted exclusion criteria. Subjects were supplemented for 1 month with 9g/day (4.5g twice a day) of a certified fermented papaya preparation. All subjects were given a stress and sleep quality questionnaire together with a diet and life style assessment. Blood was collected at 2 and 4 week, erythrocyte and leukocyte were separated to assess redox balance and heme oxygenase-1 (HO-1) gene expression while bilirubin oxidized metabolites (BOMs) were tested in the urine. Stressed individuals showed a significant abnormality of redox status with increased MDA of erythrocyte and increased level of 8-0HdG in leukocyte and BOMs excretion (p<0.05). Nutraceutical supplementation brought about a normalization of such values already at the 2 week observation (p<0.05) together with a significant upregulation of HO-1 (p<0.01). Taken together, the results of this study confirm that stressful occupational life per se, without any overt psychiatric illness, may be associated to increased oxidative stress. Supplementation with functional food affecting redox regulation may be part of the therapeutic armamentarium to be considered in this clinical setting. Copyright © by BIOLIFE, s.a.s.


Marotta F.,ReGenera Research Group | Koike K.,ORI Bioscience Laboratory | Lorenzetti A.,ReGenera Research Group | Jain S.,Urbana University | And 4 more authors.
Rejuvenation Research | Year: 2010

We tested the effect of a fermented papaya preparation (FPP; ORI, Gifu, Japan) on redox balance gene expression in 11 healthy nonsmoker, teetotaller individuals subjected to a detailed dietary and lifestyle questionnaire who refrained from any multivitamin supplement or fortified food. Redox status was assessed by erythrocyte and plasma parameters together with related leukocyte mRNA (glutathione peroxidase [GPx], superoxide dismutase [SOD], catalase, 8-oxoguanine glycosylase [hOGG1]) before/after 6 grams of FPP supplementation. At 2 and 4 weeks after FPP administration, plasma parameters remained unchanged, whereas FPP significantly upregulated all tested gene expression (p<0.05). Although posttranscriptional/translation protein modifications do occur and larger and longer studies are awaited, these preliminary data suggest that a transcriptomic modification of key redox and DNA repair genes may offer further insights when attempting to interrelate "nutragenomics" to clinical phenomena. © 2010 Mary Ann Liebert, Inc.


Jiangyang L.,General Hospital of PLA Formerly 304 Hospital | Qian L.,General Hospital of PLA Formerly 304 Hospital | Xiaohong W.,General Hospital of PLA Formerly 304 Hospital | Yi Y.,General Hospital of PLA Formerly 304 Hospital | And 5 more authors.
Acta Biomedica | Year: 2011

Immune dysfunction is associated with the onset of multiple organ dysfunction syndrome (MODS). To study the immune damage in the spleen, we observed pathological features of the spleen and investigated the number of splenic dendritic cells and T lymphocytes in MODS patients, 9 human MODS cases and 25 normal spleens were examined by light microscope, electron microscope and immunohistochemistry (S-100, CD11c, CD205, CD1a, CD80 and HLA-DR). There was resolution and dispersion of splenic corpuscles, especially white corpuscles, accompanied by apoptosis of a large amount of lymphocytes and increased number of splenic dendritic cells (DCs). CD1a +/S-100 + DCs and CD205 +/S-100 + DCs increased but the CD80 +/CD11c + DCs and CD1a +/HLA-DR +DCs decreased in MODS patients(p<0.01), CD80 +/CD11c + DCs and CD1a +/HLA-DR +DCs were mainly surrounding the remote periarterial lymphoid sheath and in red pulp. The ratio of CD4 +/CD8 + T lymphocyte declined markedly. The results indicated that at the terminal stage of MODS, the spleen was seriously damaged, the splenic DCs were inactivated and many lymphocytes were lost, especially CD4 +T, which induced T cells incapacitation and immune suppression. It is suggested that there is an important relation between changes of splenic dendritic cells and loss of lymphocyts and pathogenesis of MODS. © Mattioli 1885.


Shrivastava A.,P.A. College | Khan A.A.,P.A. College | Jain S.K.,Vikram University | Singhal P.K.,Rani Durgavati University | And 3 more authors.
Acta Biomedica | Year: 2010

L-asparaginase catalyzes the hydrolysis of L-asparagine into aspartate and ammonia, which is used as an anti-neoplastic agent. Isolation of asparaginase from microorganisms may be cardinal for producing this anticancer agent at industrial level. A total of three hundred fungal isolates were screened for L-asparaginase production. These fungal isolates were growing on various fruits and vegetables. Among these, the Fusarium sp. isolate that was growing on green chilly showed highest enzyme production. This study may give an outstanding contribution for finding organisms with high yielding L-asparaginase. Fungal L-asparaginase is superior in terms of its eukaryotic origin that may be responsible for its lesser toxicity. (www.actabiomedica.it). © Mattioli 1885.


Marotta F.,ReGenera Research Group | Catanzaro R.,University of Catania | Yadav H.,U.S. National Institutes of Health | Jain S.,U.S. National Institutes of Health | And 3 more authors.
Acta Biomedica | Year: 2012

"Functional Foods" represent an emerging opportunity and they will certainly play a consistent and important role in future too. Such a new perspective entirely depends on the growing attention paid by nutritionists to the development of new innovating solutions aiming at acting on organic systems as well as on more general topics relating to consumer good health conditions. Differently from the past, when mainly retrospective epidemiological studies or empirical experiences were carried out on single nutrients, such a new and growing interest by the scientific community follows research deeply oriented to clinics supplemented by an accurate study on nutrients, genomics and single nutritional requirement diagnostics. Already in 1993, the leading journal Nature published a report "Japan is exploring limits between food and medicine" (Swinbanks 1993). Clearly the success of "Functional Foods" depends on the food industry capacity too of developing new effective products which on the one side meet any consumer request and on the other must be have positive effects on health, supported and validated by scientific research and therefore far beyond simple positive properties, as recently underlined in a meeting, organised by a no profit non governmental international association, (www.actabiomedica.it). © Mattioli 1885.


Mariani G.,Rilevo Bioengineering Research Laboratory | Marotta F.,University of Verona | Marotta F.,ReGenera Research Group
Rejuvenation Research | Year: 2010

Most devices assessing body composition harbor a number of drawbacks and hardly assess the phenomena taking place at a cellular membrane level. The present single-frequency bioelectrical potential homeostatic structure analysis (PHoSA) technology requires only a proper hands contact on fixed electrodes and determines the phase displacement between tested current and voltage by using a 50-KHz alternate sinusoidal current. This allows quick testing time with high degree of precision, sensitivity, and specificity of sectorial functional body compartments analysis. Such assessment may prove to be an integrated part of either a diagnostic workup or monitoring tool in tailoring nutritional/ nutraceutical, pharmacological, and exercise activity, all being framed within a proactive, preventive, age-intervention management strategy. © 2010 Mary Ann Liebert, Inc.


PubMed | ReGenera Research Group
Type: Journal Article | Journal: Acta bio-medica : Atenei Parmensis | Year: 2012

Functional foods represent an emerging opportunity and they will certainly play a consistent and important role in future too. Such a new perspective entirely depends on the growing attention paid by nutritionists to the development of new innovating solutions aiming at acting on organic systems as well as on more general topics relating to consumer good health conditions. Differently from the past, when mainly retrospective epidemiological studies or empirical experiences were carried out on single nutrients, such a new and growing interest by the scientific community follows research deeply oriented to clinics supplemented by an accurate study on nutrients, genomics and single nutritional requirement diagnostics. Already in 1993, the leading journal Nature published a report Japan is exploring limits between food and medicine (Swinbanks 1993). Clearly the success of Functional foods depends on the food industry capacity too of developing new effective products which on the one side meet any consumer request and on the other must be have positive effects on health, supported and validated by scientific research and therefore far beyond simple positive properties, as recently underlined in a meeting, organised by a no profit non governmental international association.


PubMed | ReGenera Research Group
Type: Journal Article | Journal: Rejuvenation research | Year: 2010

We investigated the effect of the phytocompound Denshici-to-Chiusei (DTS) on the atherogenesis in apolipoprotein E(-/-)/low-density lipoprotein receptor(-/-) (apoE(-/-)/LDL receptor(-/-)) mice (E0). E0 mice were fed for 16 weeks with: (1) placebo or (2) 25 mg or (3) 50 mg of DTS/day. Aortic lesions were reduced by 38% (p < 0.01) in mice fed 50 mg/day, whereas peritoneal macrophages after both dosages had a 45%-60% lower (p < 0.01) capacity to oxidize LDL and to degrade it. This was associated with reduced LDL-associated lipoperoxides and a 22% inhibition (p < 0.05) in LDL aggregation. Tumor necrosis factor-alpha (TNF-alpha) expression and immunoreactivity in the aortic media increased five-fold, but this was significantly mitigated by DTS (50 mg > 25 mg) (p < 0.05). DTS significantly attenuated inflammatory mechanisms preceding atherogenesis with reduced LDL susceptibility to oxidation-aggregation.


PubMed | ReGenera Research Group
Type: Clinical Trial | Journal: Rejuvenation research | Year: 2010

We tested the effect of a fermented papaya preparation (FPP; ORI, Gifu, Japan) on redox balance gene expression in 11 healthy nonsmoker, teetotaller individuals subjected to a detailed dietary and lifestyle questionnaire who refrained from any multivitamin supplement or fortified food. Redox status was assessed by erythrocyte and plasma parameters together with related leukocyte mRNA (glutathione peroxidase [GPx], superoxide dismutase [SOD], catalase, 8-oxoguanine glycosylase [hOGG1]) before/after 6 grams of FPP supplementation. At 2 and 4 weeks after FPP administration, plasma parameters remained unchanged, whereas FPP significantly upregulated all tested gene expression (p < 0.05). Although posttranscriptional/translation protein modifications do occur and larger and longer studies are awaited, these preliminary data suggest that a transcriptomic modification of key redox and DNA repair genes may offer further insights when attempting to interrelate nutragenomics to clinical phenomena.


PubMed | ReGenera research group
Type: Journal Article | Journal: Journal of biological regulators and homeostatic agents | Year: 2011

There is increasing evidence that psychosocial stress can be viewed as a system-wide derangement of cellular homeostasis, with heightened oxidative stress and triggered proinflammatory mechanisms. The aim of this study is twofold: a) to replicate findings that psychological stress increases oxidative damage and b) to determine whether a fermented papaya preparation known to exert significant protective antioxidant properties could buffer such increases in nuclear DNA damage while also inducing epigenetic protective mechanisms. Twenty-eight sedentary men and women (age range: 28-52), who reported living a stressful lifestyle but with an overall positive attitude, were recruited for this study. Chronic diseases as well as severe burnout and use of drugs for anxiety constituted exclusion criteria. Subjects were supplemented for 1 month with 9 g/day (4.5 g twice a day) of a certified fermented papaya preparation. All subjects were given a stress and sleep quality questionnaire together with a diet and life style assessment. Blood was collected at 2 and 4 week, erythrocyte and leukocyte were separated to assess redox balance and heme oxygenase-1 (HO-1) gene expression while bilirubin oxidized metabolites (BOMs) were tested in the urine. Stressed individuals showed a significant abnormality of redox status with increased MDA of erythrocyte and increased level of 8-0HdG in leukocyte and BOMs excretion (p<0.05). Nutraceutical supplementation brought about a normalization of such values already at the 2 week observation (p<0.05) together with a significant upregulation of HO-1 (p<0.01). Taken together, the results of this study confirm that stressful occupational life per se, without any overt psychiatric illness, may be associated to increased oxidative stress. Supplementation with functional food affecting redox regulation may be part of the therapeutic armamentarium to be considered in this clinical setting.

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