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da Silva R.A.,Rede Nordeste de Biotecnologia RENORBIO | Noia N.B.,UFMA | Goncalves L.M.,University of Campinas | Pinho J.R.O.,UFMA | da Cruz M.C.F.N.,Federal University of Rio Grande do Norte
Revista Paulista de Pediatria | Year: 2013

Objective: To evaluate mothers who participated in an educational and preventive program for infants in relation to their knowledge on oral health practices. Methods: A cohort study was performed with 112 mothers and their babies aged 0 to 8 months and registered in the project "Promotion of Oral Health in Early Childhood" from the Federal University of Maranhão (Northeast Brazil). Initially, semi-structured interviews on the level of mothers' knowledge as to their babies' oral health were conducted. The oral cavities of the infants were also examined regarding: level of plaque, gingival bleeding, and dental caries. Then, educational lectures were ministered and, after a year of follow-up, new interviews and clinical examination were performed. For statistical analysis, the chi-square and Fisher exact tests were applied, being significant p<0.05. Results: Before the educational lectures, 93% of the mothers performed oral hygiene of their babies and 57.3% performed it at daytime and nighttime. After the lectures, all mothers performed the oral hygiene (p>0.02) and 74.7% performed it at daytime and nighttime (p=0.01). There were no differences regarding the consumption of cariogenic food in the initial and final questionnaires (p>0.05). Initially, 5.6% of dental surfaces had caries; 29.7%, plaques; and 11.9%, gingival bleeding. After the lectures, only 0.4% of the dental surfaces had caries (p<0.0001); 2.4%, plaque (p<0.0001); and 10.61%, gingival bleeding (p<0.0001). Conclusions: Knowledge acquisition is essential to improve oral health conditions.

Olsson D.C.,Santa Catarina State University | Pippi N.L.,Federal University of Santa Maria | Raiser A.G.,Federal University of Santa Maria | de Oliveira G.K.,Rede Nordeste de Biotecnologia RENORBIO | And 2 more authors.
Ciencia Rural | Year: 2011

The nanocrystals are quantum dots and semiconductors in aqueous solution. They have been responsible for a large volume of research, either within the basic science and which can be applied in diverse fields such as luminescent probe, among them biotechnology. The field of these quantum dots methodologies is the first and fundamental step for future applications as biomarkers in biological systems in vitro and in vivo, due to advantages over organic fluorophores. This article aims to conduct a review of biological markers nanocrystals and their importance in biological research.

Carmo R.F.,Federal University of Vale do Sao Francisco | Aroucha D.,Instituto Do Figado Of Pernambuco Ifp | Aroucha D.,University of Pernambuco | Vasconcelos L.R.S.,Instituto Do Figado Of Pernambuco Ifp | And 5 more authors.
Journal of Viral Hepatitis | Year: 2016

Hepatitis C virus (HCV) is the main cause of chronic liver disease, cirrhosis and hepatocellular carcinoma (HCC) worldwide. The risk to develop HCC increases with the severity of liver inflammation and fibrosis. Long pentraxin 3 (PTX3) is a soluble pattern-recognition receptor produced by phagocytes and nonimmune cells at sites of inflammation or injury. The aim of the present study was to determine the association of PTX3 polymorphisms and its plasma levels with HCC occurrence among patients with HCV. Samples from 524 patients with chronic hepatitis C were evaluated in this study. Two polymorphisms (rs1840680 and rs2305619) in the PTX3 gene were determined by real-time PCR. PTX3 plasma levels were measured by Enzyme-linked Immunosorbent Assay (ELISA). Our data show a significant association between PTX3 polymorphisms and HCC occurrence in univariate and multivariate analysis (P = 0.024). Patients with HCC had higher PTX3 plasma levels compared to individuals with mild or severe fibrosis (P < 0.0001 and P = 0.002, respectively). In addition, PTX3 rs2305619 polymorphism and plasma levels were correlated with Child-Pugh scores B and C in HCC individuals. PTX3 seems to be a risk factor for HCC occurrence in chronic hepatitis C. This is the first study that evaluates PTX3 in the context of hepatitis C. © 2015 John Wiley & Sons Ltd.

Mondego-Oliveira R.,State University of Maranhao | Almeida-Souza F.,Instituto Oswaldo Cruz | de Sa J.C.,University | Torres M.A.O.,Rede Nordeste de Biotecnologia RENORBIO | And 4 more authors.
Journal of Animal and Veterinary Advances | Year: 2015

The present study evaluated the effect oi Morinda citrifolia crude extract on mice infected with Leishmania amazonensis. Both infected and untreated animals displayed severe extracellular matrix destruction, hyperplasia of the white pulp of the spleen and inflammatory cell infiltration in the liver. In contrast, animals treated with M. citrifolia crude extract displayedintense extracellular matrix production, characterized by the predominance of mature collagen and no alterations to the analyzed organs. The results revealed regression of lesions caused by L. amazonensis in the groups treated with noni extract which demonstrates that M. citrifolia provides effective control of the lesions caused by the infection. © Medwell Journals, 2015.

Aroucha D.C.,Instituto Do Figado Of Pernambuco Ifp | Aroucha D.C.,University of Pernambuco | Carmo R.F.,Federal University of Vale do Sao Francisco | Vasconcelos L.R.S.,Instituto Do Figado Of Pernambuco Ifp | And 12 more authors.
Journal of Medical Virology | Year: 2016

Hepatitis C virus (HCV) is the major cause of hepatocellular carcinoma (HCC). The risk to develop HCC increases with the severity of liver inflammation and hepatic fibrosis. It is believed that a balance between the releases of pro- and anti-inflammatory cytokines will determine the clinical course of HCV and the risk to develop HCC. The inteleukin-10 (IL-10) and the tumor necrosis factor alpha (TNF-α) play key roles in the Th1 and Th2 balance during the inflammatory response against HCV. The aim of the present study was to investigate the association between polymorphisms in TNF-α -308 G>A (rs1800629), IL-10 -1082 G>A (rs1800896) and -819/-592 (rs1800871/rs1800872) with HCC risk in individuals with HCV. The present study evaluated 388 chronic HCV patients. Polymorphisms were determined by real-time PCR. Diplotypes associated with low IL-10 production and the TNF-α GG genotype were significantly associated with HCC occurrence after multivariate logistic regression analysis (P = 0.027 and P = 0.029, respectively). Additionally, the IL-10 -819 (-592) TT (AA) genotype was significantly associated with multiple nodules and HCC severity according to BCLC staging (P = 0.044 and P = 0.025, respectively). Patients carrying low production haplotypes of IL-10 and the TNF-α GG genotype have higher risk to develop HCC. J. Med. Virol. 88:1587–1595, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

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