Rede Nordeste de Biotecnologia RENORBIO

Recife, Brazil

Rede Nordeste de Biotecnologia RENORBIO

Recife, Brazil
SEARCH FILTERS
Time filter
Source Type

Carmo R.F.,Federal University of Vale do São Francisco | Aroucha D.,Instituto Do Figado Of Pernambuco Ifp | Aroucha D.,University of Pernambuco | Vasconcelos L.R.S.,Instituto Do Figado Of Pernambuco Ifp | And 5 more authors.
Journal of Viral Hepatitis | Year: 2016

Hepatitis C virus (HCV) is the main cause of chronic liver disease, cirrhosis and hepatocellular carcinoma (HCC) worldwide. The risk to develop HCC increases with the severity of liver inflammation and fibrosis. Long pentraxin 3 (PTX3) is a soluble pattern-recognition receptor produced by phagocytes and nonimmune cells at sites of inflammation or injury. The aim of the present study was to determine the association of PTX3 polymorphisms and its plasma levels with HCC occurrence among patients with HCV. Samples from 524 patients with chronic hepatitis C were evaluated in this study. Two polymorphisms (rs1840680 and rs2305619) in the PTX3 gene were determined by real-time PCR. PTX3 plasma levels were measured by Enzyme-linked Immunosorbent Assay (ELISA). Our data show a significant association between PTX3 polymorphisms and HCC occurrence in univariate and multivariate analysis (P = 0.024). Patients with HCC had higher PTX3 plasma levels compared to individuals with mild or severe fibrosis (P < 0.0001 and P = 0.002, respectively). In addition, PTX3 rs2305619 polymorphism and plasma levels were correlated with Child-Pugh scores B and C in HCC individuals. PTX3 seems to be a risk factor for HCC occurrence in chronic hepatitis C. This is the first study that evaluates PTX3 in the context of hepatitis C. © 2015 John Wiley & Sons Ltd.


PubMed | Instituto Do Figado Of Pernambuco Ifp, Instituto Oswaldo Cruz, Federal University of Vale do São Francisco, Rede Nordeste de Biotecnologia RENORBIO and 2 more.
Type: Journal Article | Journal: Journal of medical virology | Year: 2016

Hepatitis C virus (HCV) is the major cause of hepatocellular carcinoma (HCC). The risk to develop HCC increases with the severity of liver inflammation and hepatic fibrosis. It is believed that a balance between the releases of pro- and anti-inflammatory cytokines will determine the clinical course of HCV and the risk to develop HCC. The inteleukin-10 (IL-10) and the tumor necrosis factor alpha (TNF-) play key roles in the Th1 and Th2 balance during the inflammatory response against HCV. The aim of the present study was to investigate the association between polymorphisms in TNF- -308 G>A (rs1800629), IL-10 -1082 G>A (rs1800896) and -819/-592 (rs1800871/rs1800872) with HCC risk in individuals with HCV. The present study evaluated 388 chronic HCV patients. Polymorphisms were determined by real-time PCR. Diplotypes associated with low IL-10 production and the TNF- GG genotype were significantly associated with HCC occurrence after multivariate logistic regression analysis (P=0.027 and P=0.029, respectively). Additionally, the IL-10 -819 (-592) TT (AA) genotype was significantly associated with multiple nodules and HCC severity according to BCLC staging (P=0.044 and P=0.025, respectively). Patients carrying low production haplotypes of IL-10 and the TNF- GG genotype have higher risk to develop HCC. J. Med. Virol. 88:1587-1595, 2016. 2016 Wiley Periodicals, Inc.


Costa D.N.M.,Federal University of Piauí | Martiliano Silva D.A.M.,Federal University of Piauí | Boakari Y.L.,Federal University of Piauí | Ferreira S.B.,Federal University of Piauí | And 2 more authors.
Ciencia Animal Brasileira | Year: 2014

The limiting factor in the cryopreservation process of equine semen is related to the species, as they present a great variability in the ejaculate's characteristics after thawing. The aim of this study was to evaluate sperm viability after thawing, from Quarter Horse and Mangalarga Marchador stallions using two extenders (Botu-crio and Tris) in cryopreservation. To this end, we analyzed the physical characteristics of fresh semen, the sperm membrane functionality by the hypoosmotic swelling test (HOST), total motility and vigor by the Thermoresistance Test (TRT), progressive motility by a computerized system CASA (Computer-Assisted Semen Anlyses) and acrosomal membrane integrity and functionality of the mitochondria by epifluorescence microscopy. After thawing, the extender Botu-crio® better preserved motility, vigor and integrity of the plasma membrane. There was no significant difference between breeds for the thermoresistance test after twaing. Quarter Horse showed higher percentage of bigger defects in the sperm pathology analysis.


da Silva R.A.,Rede Nordeste de Biotecnologia Renorbio | Noia N.B.,UFMA | Goncalves L.M.,University of Campinas | Pinho J.R.O.,UFMA | da Cruz M.C.F.N.,Federal University of Rio Grande do Norte
Revista Paulista de Pediatria | Year: 2013

Objective: To evaluate mothers who participated in an educational and preventive program for infants in relation to their knowledge on oral health practices. Methods: A cohort study was performed with 112 mothers and their babies aged 0 to 8 months and registered in the project "Promotion of Oral Health in Early Childhood" from the Federal University of Maranhão (Northeast Brazil). Initially, semi-structured interviews on the level of mothers' knowledge as to their babies' oral health were conducted. The oral cavities of the infants were also examined regarding: level of plaque, gingival bleeding, and dental caries. Then, educational lectures were ministered and, after a year of follow-up, new interviews and clinical examination were performed. For statistical analysis, the chi-square and Fisher exact tests were applied, being significant p<0.05. Results: Before the educational lectures, 93% of the mothers performed oral hygiene of their babies and 57.3% performed it at daytime and nighttime. After the lectures, all mothers performed the oral hygiene (p>0.02) and 74.7% performed it at daytime and nighttime (p=0.01). There were no differences regarding the consumption of cariogenic food in the initial and final questionnaires (p>0.05). Initially, 5.6% of dental surfaces had caries; 29.7%, plaques; and 11.9%, gingival bleeding. After the lectures, only 0.4% of the dental surfaces had caries (p<0.0001); 2.4%, plaque (p<0.0001); and 10.61%, gingival bleeding (p<0.0001). Conclusions: Knowledge acquisition is essential to improve oral health conditions.


Mondego-Oliveira R.,State University of Maranhão | Almeida-Souza F.,Instituto Oswaldo Cruz | de Sa J.C.,University CEUMA | Torres M.A.O.,Rede Nordeste de Biotecnologia RENORBIO | And 4 more authors.
Journal of Animal and Veterinary Advances | Year: 2015

The present study evaluated the effect oi Morinda citrifolia crude extract on mice infected with Leishmania amazonensis. Both infected and untreated animals displayed severe extracellular matrix destruction, hyperplasia of the white pulp of the spleen and inflammatory cell infiltration in the liver. In contrast, animals treated with M. citrifolia crude extract displayedintense extracellular matrix production, characterized by the predominance of mature collagen and no alterations to the analyzed organs. The results revealed regression of lesions caused by L. amazonensis in the groups treated with noni extract which demonstrates that M. citrifolia provides effective control of the lesions caused by the infection. © Medwell Journals, 2015.


Martins N.S.,Rede Nordeste de Biotecnologia RENORBIO | Coelho G.B.,State University of Maranhão | dos Santos L.S.,Rede Norte de Biotecnologia BIONORTE | de Sousa R.A.,State University of Maranhão | And 2 more authors.
Ciencia Animal Brasileira | Year: 2015

The aim of this study was to study the changes in the splenic extracellular matrix of dogs naturally infected with Leishmania (Leishmania) infantum chagasi and its correlation to clinical, histopathological, and parasitological aspects. Eighteen dogs were used, separated into three groups: six non-infected animals (control group) and twelve infected animals. The dogs had undefined breed and age, from the township region of São Luís-MA. Paraffined slices of the spleen were stained with Hematoxilin and Eosin (H&E); Gomori's ammoniacal Silver, to mark reticular fibers; and the Immunohistochemistry technique of streptavidin peroxidase to detect amastigote forms of Leishmania. The morphometrical analyses were done using the KS300 program and the images analysis system Kontron Elektronic/Carl Zeiss, Germany. The results showed that there is a significant increase in the deposition of collagen fibers in the spleen, compared to control animals, showing significant differences for symptomatic and asymptomatic animals. Positive correlations were found between the presence of the parasite in the tissue and collagen deposition. Symptomatic animals showed higher collagen deposition in the spleen, which can be associated to the high parasitism found in the tissue. The results showed that there is an intense fibrogenesis in the spleen in the canine visceral leishmaniasis, thus being associated to the parasitism of the tissue and the degenerative processes of the disease.


Santos O.J.D.,Rede Nordeste de Biotecnologia RENORBIO | Santos O.J.D.,Federal University of Maranhão | Filho E.N.S.,Rede Nordeste de Biotecnologia RENORBIO | Filho E.N.S.,Federal University of Maranhão | And 5 more authors.
Journal of Coloproctology | Year: 2016

Objectives: To assess the epidemiological profile of children and adolescents with intestinal stoma, assisted at the Presidente Dutra University Hospital (HUPD), São Luís (MA). Methods: Observational, descriptive, retrospective, and individuated study. Data were collected from 110 children and adolescents with elimination intestinal stoma, from January 2006 to February 2013. The following variables were analyzed: age, gender, patient origin, and stomata indication, type, and temporal character. After data collection, descriptive analysis was made by Bioestat 5.0 program. Results: Of 110 patients, 78.2% were male and 21.8% female. The average age was 9.4 years old. 55.5% came from the Maranhão state countryside, and 44.5% from the capital, São Luís. Regarding stoma type, colostomies made up 88.2%, and were 11.8% ileostomies, all temporary stoma. The main cause was trauma (42.7%), with firearm abdominal puncture being the most frequent cause (71.5% of the category); followed by congenital anomalies (38.2%), and obstructive (5.4%) and inflammatory (4.5%) causes. Conclusion: Studied children and adolescents were mostly male, from Maranhão state countryside. The main cause was trauma, and colostomy was the most common stoma type. © 2016 Published by Elsevier Editora Ltda.


Rodrigues J.A.G.,Rede Nordeste de Biotecnologia RENORBIO | de Araujo I.W.F.,Rede Nordeste de Biotecnologia RENORBIO | de Paula G.A.,Rede Nordeste de Biotecnologia RENORBIO | Vanderlei E.S.O.,Federal University of Ceará | And 6 more authors.
Ciencia Rural | Year: 2011

This study aimed to isolate, fractionate and evaluate the in vivo toxicity of sulfated polysaccharides (SPs) from Hypnea musciformis (Rhodophyta), when obtained by three extraction methods (M I, M II and M III). SPs were extracted with papain in 100mM sodium acetate (pH 5.0) containing cysteine and EDTA (5mM) (M I) or water (25-80°C (M II), 80°C (M III)), and then their chemical composition of total carbohydrates, free sulfate (FS) and contaminant proteins (CPs) was determined. SPs were submitted to ion-exchange chromatography (DEAE-celulose) using a sodium chloride gradient, being the degree of homogeneity and charge density evaluated by agarose gel electrophoresis of the fractions obtained and compared to heparin. The in vivo assay was performed using groups (n=6) of male and female Swiss mice (24-33g), which received: SPs (9mg kg -1, i.p.) absence of CPs (M I) and 0.9% saline (0.1mL 10g -1, i.p.), for 14 consecutive days. On the 15 th day, collect blood and organs for biochemical dosages and corporal mass correlation, respectively, from the animals anesthetized and sacrificed were performed. The sulfate content of FS (31.05±0.53%) (P<0.05) and the fractionation by DEAE-cellulose showed M I more effectiveness in obtaining SPs compared to M II and M III. The animals were tolerable to SPs from M I, and it wasn't observed hepatic or renal alteration (P>0.05).


Chaves N.P.,Rede Nordeste de Biotecnologia RENORBIO | Bezerra D.C.,Rede Nordeste de Biotecnologia RENORBIO | Santos H.P.,State University of Maranhão | De Moraes Perreira H.,State University of Maranhão | And 2 more authors.
Ciencia Animal Brasileira | Year: 2014

The objective of this study was to determine the occurrence and risk factors associated with the identification of equine infectious anemia (EIA) in traction equines in the city of São Luiz - MA. We collected blood samples from 154 equines seized on public roads by the Sector of Animal Traction Seizure of the Municipal Urban Development and Housing Department between April and August of 2011. We analyzed the medical records to determine the individual risk factors associated with this disease. Of the animals tested, 5.20% (8/154) were positive. Among the risk factors evaluated, the presence of insects, permanence of the animals in agglomerations for longer than 8 hours per day, body condition, nutritional status, repeated use of the same needle among different equines, occurrence of febrile episodes, yellowish mucous and lack of knowledge on IEA showed to be statistically significant (P <0.05) associated with the IEA. The study results indicate that the occurrence of the IEA in traction equines in the City of São Luiz - MA was low. Still there is the necessity of systematic diagnosis and monitoring of animals, as well as employment of control measures, serological tests and implementation of current legislation.


PubMed | Federal University of Vale do São Francisco, Instituto Do Figado Of Pernambuco Ifp, Rede Nordeste de Biotecnologia RENORBIO and University of Pernambuco
Type: Journal Article | Journal: Journal of viral hepatitis | Year: 2016

Hepatitis C virus (HCV) is the main cause of chronic liver disease, cirrhosis and hepatocellular carcinoma (HCC) worldwide. The risk to develop HCC increases with the severity of liver inflammation and fibrosis. Long pentraxin 3 (PTX3) is a soluble pattern-recognition receptor produced by phagocytes and nonimmune cells at sites of inflammation or injury. The aim of the present study was to determine the association of PTX3 polymorphisms and its plasma levels with HCC occurrence among patients with HCV. Samples from 524 patients with chronic hepatitis C were evaluated in this study. Two polymorphisms (rs1840680 and rs2305619) in the PTX3 gene were determined by real-time PCR. PTX3 plasma levels were measured by Enzyme-linked Immunosorbent Assay (ELISA). Our data show a significant association between PTX3 polymorphisms and HCC occurrence in univariate and multivariate analysis (P = 0.024). Patients with HCC had higher PTX3 plasma levels compared to individuals with mild or severe fibrosis (P < 0.0001 and P = 0.002, respectively). In addition, PTX3 rs2305619 polymorphism and plasma levels were correlated with Child-Pugh scores B and C in HCC individuals. PTX3 seems to be a risk factor for HCC occurrence in chronic hepatitis C. This is the first study that evaluates PTX3 in the context of hepatitis C.

Loading Rede Nordeste de Biotecnologia RENORBIO collaborators
Loading Rede Nordeste de Biotecnologia RENORBIO collaborators