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São Luís, Brazil

Rodrigues J.A.G.,Rede Nordeste de Biotecnologia | de Sousa Oliveira Vanderlei E.,Federal University of Ceara | Quindere A.L.G.,Federal University of Ceara | Fontes B.P.,Federal University of Ceara | And 2 more authors.
Acta Scientiarum - Biological Sciences | Year: 2012

A great number of pharmacological compounds is found in the Brazilian marine diversity. This study evaluated the anticoagulant potential of glycosaminoglycans (GAGs) isolated from the skin of 'palombeta' Chloroscombrus chrysurus and 'guaiúba' Ocyurus chrysurus. GAGs were extracted with crude papain in 0.1 M sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed by ion exchange chromatography on DEAE-cellulose column. The chemical composition (contaminant proteins and total carbohydrates) and the analysis by 0.5% agarose gel electrophoresis of fractions were also determined. Anticoagulant assays were performed by activated partial thromboplastin time (APTT) using normal human plasma and standard heparin (193.00 IU mg -1). The obtaining and fractionation procedures of GAGs were effective and similar chromatographic profiles were verified between the species. A similar mobility to dermatan sulfate was revealed for C. chrysurus. This GAG also showed a low activity of 3.30 IU mg -1. Source


Rodrigues J.A.G.,Rede Nordeste de Biotecnologia | Vanderlei E.S.O.,Federal University of Ceara | Quindere A.L.G.,Federal University of Ceara | Fontes B.P.,Federal University of Ceara | Benevides N.M.B.,Federal University of Ceara
Acta Scientiarum - Biological Sciences | Year: 2010

The incidence of cardiovascular diseases and adverse effects from heparintherapy have led to a search for new therapeutic agents, and the sulfated polysaccharides (SP) of seaweeds have been reported as alternative sources. The aim of this work was to evaluate the anticoagulant potential of total SP (TSP) isolated and fractionated from Caulerpa racemosa and Caulerpa cupressoides (Chlorophyceaes). Initially, the TSP were extracted with papain in 0.1 M sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed by fractionation on ion exchange DEAE-cellulose column with NaCl gradient. The obtained fractions were analyzed by 0.5% agarose gel electrophoresis and the anticoagulant activity measured by the activated partial thromboplastin time (APTT) using normal human plasma, and compared to a standard heparin curve (193 IU mg -1). Similar chromatographic profiles of SP were shown on both species, but with distinct mobility patterns, when the SP fractions were compared by electrophoresis. SP eluted with 0.75 M of NaCl modified the APTT, whose anticoagulant activities were only 21.23 and 24.36 IU mg -1 for C. racemosa and C. cupressoides, respectively. Therefore, anticoagulant SP isolated from chlorophyceaes showed effects inferior to heparin, and comparative studies of these molecules are also suggested as auxiliary tools in the identification of algae of the same genus. Source


Neto V.S.G.,Rede Nordeste de Biotecnologia | Neto V.S.G.,Visa | Filho A.K.D.B.,Rede Nordeste de Biotecnologia | Filho A.K.D.B.,Federal University of Maranhao | And 5 more authors.
Revista da Sociedade Brasileira de Medicina Tropical | Year: 2013

Introduction: The incidence of American cutaneous leishmaniasis (ACL) is increasing in Latin America, especially in Brazil, where 256,587 cases were confirmed in the last decade. Methods: This study used a Bayesian model to examine the spatial and temporal distribution of ACL cases between 2000 and 2009 in 61 counties of State of Maranhão located along the three main road and railway corridors. Results: During the study period, 13,818 cases of ACL were recorded. There was a significant decrease in the incidence of ACL in the ten study years. The recorded incidence rate ranged from 7.36 to 241.45 per 100,000 inhabitants. The relative risk increased in 77% of the counties, decreased in 18% and was maintained in only five counties. Conclusions: Although there was a decreased incidence of the disease, ACL was present in all of the examined municipalities, thus maintaining the risk of contracting this illness. Source


Rodrigues J.A.G.,Rede Nordeste de Biotecnologia | de Queiroz I.N.L.,Federal University of Ceara | Quindere A.L.G.,Federal University of Ceara | Vairo B.,Federal University of Rio de Janeiro | And 3 more authors.
Ciencia Rural | Year: 2011

Red algae sulfated polysaccharides (SPs) have been widely described as anticoagulant and antithrombotic agents; however no description of antithrombotic activity regarding green algae SPs has been reported. Caulerpa cupressoides (Chlorophyta) has three different SPs fractions (SP 1, SP 2 and SP 3). We investigated the effects of SP2 on thrombin activity by antithrombin and in an experimental model of venous thrombosis in rats. The inhibition of thrombin assay was evaluated using antithrombin (AT) in the presence of SP 2 and the antithrombotic activity was investigated in rats with thromboplastin as the thrombogenic stimulus. The anticoagulant effects of SP 2 are suggested be due to the potentiation of thrombin inhibition by antithrombin (IC 50 ~ 10.0μg mL -1) and this mechanism of interaction is different when compared to other studied Caulerpa polysaccharides. SP 2 exhibited antithrombotic effects at doses of 1.0 and 2.0mg kg-1 body weight, but at higher doses (>2.0mg kg -1 body weight) this polysaccharide revert the antithrombotic property. No hemorrhagic effect (2.0mg kg -1) was observed. As occurs with red algae SPs, these results indicate that green algae SPs are also capable of exhibiting different in vivo properties. Source


Rodrigues J.A.G.,Rede Nordeste de Biotecnologia | de Araujo I.W.F.,Rede Nordeste de Biotecnologia | de Paula G.A.,Rede Nordeste de Biotecnologia | Bessa E.F.,Federal University of Ceara | And 2 more authors.
Ciencia Rural | Year: 2010

This study aimed to isolate, fractionate and evaluate the anticoagulant potential of iota-carrageenans (i-CARs) from Solieria filiformis when two extraction methods (M I and M II) were used. i-CARs were isolated with papain in 0.1M sodium acetate (pH 5.0) containing 5mM cystein and 5mM EDTA (M I) or water (80°C) (M II), and then their chemical composition of total carbohydrates, free sulfate (FS) and contaminant proteins were determined. i-CARs were submitted to anionexchange chromatography (DEAE-cellulose) using a sodium chloride gradient,being evaluated the activated partial thromboplastin time (APTT) and prothrombin time of obtained fractions and compared to heparin (193IU mg-1). A rich fraction of anticoagulant was also submitted to 0.5% agarose gel electrophoresis procedure. The difference of yield between methods was 10.14%. The chemical composition of FS (29.40%) and the fractionation by DEAE-cellulose showed M I more effectiveness in the obtaining of i-CARs compared to M II. The APTT was also modified for i-CARs from M I. However, the in vitro anticoagulant activity of a rich fraction (8.52IU mg-1) was inferior to heparin. Source

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