Rede Nordeste de Biotecnologia

São Luís, Brazil

Rede Nordeste de Biotecnologia

São Luís, Brazil
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Dantas M.G.B.,Federal University of Vale do São Francisco | Damasceno C.M.D.,Center for Studies and Research of Medicinal Plants | De Barros V.R.P.,Rede Nordeste de Biotecnologia | Menezes E.S.,University of Campinas | And 4 more authors.
Acta Cirurgica Brasileira | Year: 2017

Purpose: To realize a morphological examination of the musculoskeletal tissue, assessing the effect of a contusion method for the production in rat gastrocnemius, comparing the inflammatory responses generated by different impacts. Methods: For the analysis of a contusion method, twelve female Wistar rats were distributed into four groups. The lesion was generated by 324 g of mass that was dropped from different predetermined heights for each group (30, 45, 60 and 70 cm). Results: In the analysis of musculoskeletal tissue, the response to injury varied according to the mass of the height drop onto the muscle. Only the group that was injured from 70 cm responded with uniform and severe inflammation, whereas the groups 30, 45 and 60 cm showed inflammation in some regions of the tissue with mild and moderate infiltrates. Conclusion: The method with the 324-gram mass dropped from a 70-cm height onto the gastrocnemius muscle of rats seems to be the most suitable for the production of muscle injury in these animals after 72 hours, showing an important inflammatory infiltrate. © 2017, Sociedade Brasileira para o Desenvolvimento de Pesquisa em Cirurgia. All rights reserved.


Macedo I.T.F.,State University of Ceará | Bevilaqua C.M.L.,State University of Ceará | de Oliveira L.M.B.,State University of Ceará | Camurca-Vasconcelos A.L.F.,State University of Ceará | And 3 more authors.
Veterinary Parasitology | Year: 2012

The resistance of gastrointestinal nematodes to anthelmintics has increased the need to evaluate natural products that can replace or assist current strategies to control gastrointestinal nematodes. The objective of this study was to evaluate the effect of decoctions of Lantana camara (DLc), Alpinia zerumbet (DAz), Mentha villosa (DMv) and Tagetes minuta (DTm) on Haemonchus contortus by two in vitro tests. The effects of increasing concentrations of lyophilized decoctions (0.31 to 10. mg/ml) were assessed using the egg hatch test (EHT). The decoctions were then tested in the larval artificial exsheathment assay. H. contortus third stage larvae (L3) were exposed to 0.31. mg/ml A. zerumbet and M. villosa decoctions and 0.62. mg/ml T. minuta and L. camara decoctions for 3. h and then exsheathment procedure at 10. min intervals. An inhibitor of tannins, polyvinyl polypyrrolidone (PVPP), was used to study if tannins were responsible for the inhibitory effect on hatching and exsheathment of larvae. A. zerumbet, M. villosa and T. minuta showed a dose-dependent effect in the EHT, which did not disappear after the addition of PVPP. No effect was observed for L. camara in the EHT. However, the decoctions inhibited the process of larval exsheathment, which may be related to tannin action because the addition of PVPP reversed the inhibitory effect. A. zerumbet, M. villosa and T. minuta decoctions showed inhibitory activity on H. contortus larvae hatching and exsheathing. The decoctions of these plants could be used to control gastrointestinal nematodes following confirmation of their anthelmintic activity in vivo. © 2012 Elsevier B.V.


PubMed | Rede Nordeste de Biotecnologia, Institute Medicina Integral Professor Fernando Figueira, Federal University of Pernambuco and University of Pernambuco
Type: Journal Article | Journal: Memorias do Instituto Oswaldo Cruz | Year: 2016

Human papillomavirus (HPV) infections are strongly associated with the development of cervical intraepithelial neoplasias and invasive cervical cancer. Polymorphisms in cytokine-encoding genes and behavioural cofactors could play an important role in protecting an individual against viral infections and cancer. Here, we investigated whether IL-6 -174 G>C, IL-8 +396 G>T, and TGF-1 +869 G>C and +915 G>C polymorphisms were associated with susceptibility to HPV infection in women from north-east (Pernambuco) Brazil. We analysed 108 healthy uninfected women (HC) and 108 HPV-positive women with cervical lesions. Genetic polymorphisms were assessed using Sanger sequencing and polymerase chain reaction-restriction fragment length polymorphism. Comparison of the distribution of the genotypic and allelic frequencies of the IL-18 +396 T>G polymorphism between HPV infected woman an uninfected controls showed that the GG genotype and G allele were both more frequent in the HC group, and were associated with protection from HPV infection (p = 0.0015; OR = 0.29 CI95% = 0.13-0.61; p = 0.0005; OR = 0.45 CI95% 0.29-0.7, respectively). Individuals from the control group could have previously had HPV infection that was spontaneously eliminated; however, it was undetectable at the time of sample collection. Based on our findings, we hypothesize that the IL-8 +396 G>T polymorphism could interfere with susceptibility to HPV infection, by modulating the ability of immune system to fight the virus.


do Carmo R.F.,Federal University of Vale do São Francisco | Vasconcelos L.R.S.,Instituto Do Figado Of Pernambuco | Mendonca T.F.,Rede Nordeste de Biotecnologia | de Mendonca Cavalcanti M.D.S.,University of Pernambuco | And 3 more authors.
Human Immunology | Year: 2014

Oxidative stress plays an important role on liver fibrosis progression in the course of hepatitis C virus (HCV) infection. Myeloperoxidase (MPO) is an enzyme released by neutrophils and macrophages, responsible for generating hypochlorous acid and reactive oxygen species (ROS) that may lead to liver injury in HCV infection. On the other hand, antioxidant enzymes such as manganese superoxide dismutase (SOD) controls ROS-mediated damage. The aim of the present study was to investigate the influence of MPO G-463A and SOD2 Ala16Val polymorphisms in the severity of liver fibrosis in individuals with chronic HCV infection. The present study included 270 patients with chronic HCV recruited from the Gastrohepatology Service of the Oswaldo Cruz University Hospital/Liver Institute of Pernambuco (Recife, Northeastern Brazil). All patients underwent liver biopsy, which was classified according METAVIR score. The SNPs were determined by real-time PCR. After multivariate analysis adjustment, the GG genotype of MPO and the presence of metabolic syndrome were independently associated with fibrosis severity in women (P= 0.025 OR 2.25 CI 1.10-4.59 and P= 0.032 OR 2.32 CI 1.07-5.01, respectively). The presence of the GG genotype seems to be a risk factor for fibrosis severity in women with HCV. © 2014 American Society for Histocompatibility and Immunogenetics.


Neto V.S.G.,Rede Nordeste de Biotecnologia | Neto V.S.G.,Visa | Filho A.K.D.B.,Rede Nordeste de Biotecnologia | Filho A.K.D.B.,Federal University of Maranhão | And 5 more authors.
Revista da Sociedade Brasileira de Medicina Tropical | Year: 2013

Introduction: The incidence of American cutaneous leishmaniasis (ACL) is increasing in Latin America, especially in Brazil, where 256,587 cases were confirmed in the last decade. Methods: This study used a Bayesian model to examine the spatial and temporal distribution of ACL cases between 2000 and 2009 in 61 counties of State of Maranhão located along the three main road and railway corridors. Results: During the study period, 13,818 cases of ACL were recorded. There was a significant decrease in the incidence of ACL in the ten study years. The recorded incidence rate ranged from 7.36 to 241.45 per 100,000 inhabitants. The relative risk increased in 77% of the counties, decreased in 18% and was maintained in only five counties. Conclusions: Although there was a decreased incidence of the disease, ACL was present in all of the examined municipalities, thus maintaining the risk of contracting this illness.


Rodrigues J.A.G.,Rede Nordeste de Biotecnologia | de Queiroz I.N.L.,Federal University of Ceará | Quindere A.L.G.,Federal University of Ceará | Vairo B.,Federal University of Rio de Janeiro | And 3 more authors.
Ciencia Rural | Year: 2011

Red algae sulfated polysaccharides (SPs) have been widely described as anticoagulant and antithrombotic agents; however no description of antithrombotic activity regarding green algae SPs has been reported. Caulerpa cupressoides (Chlorophyta) has three different SPs fractions (SP 1, SP 2 and SP 3). We investigated the effects of SP2 on thrombin activity by antithrombin and in an experimental model of venous thrombosis in rats. The inhibition of thrombin assay was evaluated using antithrombin (AT) in the presence of SP 2 and the antithrombotic activity was investigated in rats with thromboplastin as the thrombogenic stimulus. The anticoagulant effects of SP 2 are suggested be due to the potentiation of thrombin inhibition by antithrombin (IC 50 ~ 10.0μg mL -1) and this mechanism of interaction is different when compared to other studied Caulerpa polysaccharides. SP 2 exhibited antithrombotic effects at doses of 1.0 and 2.0mg kg-1 body weight, but at higher doses (>2.0mg kg -1 body weight) this polysaccharide revert the antithrombotic property. No hemorrhagic effect (2.0mg kg -1) was observed. As occurs with red algae SPs, these results indicate that green algae SPs are also capable of exhibiting different in vivo properties.


Rodrigues J.A.G.,Rede Nordeste de Biotecnologia | de Araujo I.W.F.,Rede Nordeste de Biotecnologia | de Paula G.A.,Rede Nordeste de Biotecnologia | Bessa E.F.,Federal University of Ceará | And 2 more authors.
Ciencia Rural | Year: 2010

This study aimed to isolate, fractionate and evaluate the anticoagulant potential of iota-carrageenans (i-CARs) from Solieria filiformis when two extraction methods (M I and M II) were used. i-CARs were isolated with papain in 0.1M sodium acetate (pH 5.0) containing 5mM cystein and 5mM EDTA (M I) or water (80°C) (M II), and then their chemical composition of total carbohydrates, free sulfate (FS) and contaminant proteins were determined. i-CARs were submitted to anionexchange chromatography (DEAE-cellulose) using a sodium chloride gradient,being evaluated the activated partial thromboplastin time (APTT) and prothrombin time of obtained fractions and compared to heparin (193IU mg-1). A rich fraction of anticoagulant was also submitted to 0.5% agarose gel electrophoresis procedure. The difference of yield between methods was 10.14%. The chemical composition of FS (29.40%) and the fractionation by DEAE-cellulose showed M I more effectiveness in the obtaining of i-CARs compared to M II. The APTT was also modified for i-CARs from M I. However, the in vitro anticoagulant activity of a rich fraction (8.52IU mg-1) was inferior to heparin.


Rodrigues J.A.G.,Rede Nordeste de Biotecnologia | Vanderlei E.S.O.,Federal University of Ceará | Quindere A.L.G.,Federal University of Ceará | Fontes B.P.,Federal University of Ceará | Benevides N.M.B.,Federal University of Ceará
Acta Scientiarum - Biological Sciences | Year: 2010

The incidence of cardiovascular diseases and adverse effects from heparintherapy have led to a search for new therapeutic agents, and the sulfated polysaccharides (SP) of seaweeds have been reported as alternative sources. The aim of this work was to evaluate the anticoagulant potential of total SP (TSP) isolated and fractionated from Caulerpa racemosa and Caulerpa cupressoides (Chlorophyceaes). Initially, the TSP were extracted with papain in 0.1 M sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed by fractionation on ion exchange DEAE-cellulose column with NaCl gradient. The obtained fractions were analyzed by 0.5% agarose gel electrophoresis and the anticoagulant activity measured by the activated partial thromboplastin time (APTT) using normal human plasma, and compared to a standard heparin curve (193 IU mg -1). Similar chromatographic profiles of SP were shown on both species, but with distinct mobility patterns, when the SP fractions were compared by electrophoresis. SP eluted with 0.75 M of NaCl modified the APTT, whose anticoagulant activities were only 21.23 and 24.36 IU mg -1 for C. racemosa and C. cupressoides, respectively. Therefore, anticoagulant SP isolated from chlorophyceaes showed effects inferior to heparin, and comparative studies of these molecules are also suggested as auxiliary tools in the identification of algae of the same genus.


Rodrigues J.A.G.,Rede Nordeste de Biotecnologia | de Araujo I.W.F.,Rede Nordeste de Biotecnologia | de Paula G.A.,Rede Nordeste de Biotecnologia | Lima T.B.,Federal University of Ceará | And 2 more authors.
Acta Scientiarum - Technology | Year: 2011

Solieria filiformis (Rhodophyceae) culture is being developed at Flecheiras Beach, state of Ceará, Brazil, for its future sustainable use in biotechnology. However, epiphyte growth on the species jeopardizes suitable development. The efficiency of two methods (M I and M II) in the isolation of kappa-carrageenan (κ-CAR) from red marine alga Hypnea musciformis has been evaluated. κ-CAR was obtained by proteolytic digestion (M I) or by hot water (80°C) (M II) and extracts were analyzed with regard to their chemical composition and then by ion-exchange chromatography on DEAE-cellulose column with NaCl gradient. Anticoagulant activity was evaluated by the activated partial thromboplastine time (APTT) test with common human plasma and standard heparin (HEP) (193 IU mg-1). Yield difference reached 4.28 ± 0.26%% (p < 0.05), and the lowest sulfate content was obtained with M I (17.00%) when compared to that with M II (26.30%). Further, different chromatographic profiles were reported between the two methods. Whereas M I was efficacious in obtaining κ-CAR anticoagulants (2.67 IU mg-1), alternative M II was efficient for the food industry. Results suggest that κ-CAR extraction method from H. musciformis depends on the industry's usage criterion.


PubMed | Rede Nordeste de Biotecnologia, UFMA and Federal University of Maranhão
Type: Journal Article | Journal: Ciencia & saude coletiva | Year: 2016

A cross-sectional population-based study using questionnaire and anthropometric data was conducted on 968 university students of So Lus, Brazil, from which 590 showed up for blood collection. In the statistical analysis the Student t-test, Mann-Whitney and chi-square tests were used. The prevalence of metabolic syndrome by the Joint Interim Statement (JIS) criteria was 20.5%, almost three times more prevalent in men (32.2%) than in women (13.5%) (P < 0.001). The prevalence of insulin resistance was 7.3% and the prevalence of low HDL-cholesterol was high (61.2%), both with no statistically significant differences by sex. Men showed a higher percentage of smoking, overweight, high blood pressure, high blood glucose and increased fasting hypertriglyceridemia. Women were more sedentary. University students of private institutions had higher prevalences of sedentary lifestyle, obesity, abdominal obesity, elevated triglycerides and metabolic syndrome than students from public institutions. High prevalences of metabolic syndrome, insulin resistance and other cardiovascular risk factors were found in this young population. This suggests that the burden of these diseases in the future will be increased.

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