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Stevens Point, NV, United States

Phan V.,Red Rock Fertility Center | Littman E.,Red Rock Fertility Center | Harris D.,Red Rock Fertility Center | La A.,Red Rock Fertility Center
Asian Pacific Journal of Reproduction | Year: 2014

Objective: To carry out a detailed investigation of the impact of aneuploidy on blastocyst quality. Methods: 1 257 cleavage-stage embryos from 203 patients underwent IVF treatment with aneuploidy screening were investigated. Comprehensive chromosome analysis involved the use of microarray comparative genomic hybridisation (aCGH) method on single blastomere biopsied on day 3 of embryo development. Embryo morphology was assessed and recorded on days 3 and/or 5-6 post-fertilisation. Morphologic grade A-C was assigned to the blastocysts with grade A being the highest grade and grade C the lowest. Results: Among the 1 257 embryos that were analyzed, 474 (38%) were euploid, and 783 (62%) were aneuploid. 315/474 (66.5%) of euploid embyos developed into blastocysts, while 57/783 (32.8%) of aneuploid embryos grew to blastocysts (P<0.01). At the blastocyst stage, the best quality embryos (grade A) were in their majority (164/241 or 68%) euploid. Of the 219 fair quality blastocyts (grade B) 129 were euploid (59%) and 22/112 (20%) of poor quality blastocysts (grade C) were euploid. Conclusion: Blastocyst morphology showed a significant link to aneuploidy (P<0.05). However, grading morphology alone can not replace preimplantation aneuploidy screening. Morphology screening or other markers for embryo competence, combined with preimplantation genetic screening for 24 chromosomes may produce the best results. © 2014 Hainan Medical College. Source


Phan V.,Red Rock Fertility Center | Littman E.,Red Rock Fertility Center | Harris D.,Red Rock Fertility Center | La A.,Red Rock Fertility Center
Asian Pacific Journal of Reproduction | Year: 2014

Objective: To analyze the correlation between embryo morphology and the chromosomal status using the array comparative genomic hybridization [array comparative genomic hybridization (a-CGH)] technique for screening 23 chromosome pairs in a single blastomere biopsy from Day 3 embryos. Methods: One thousand five hundred and fifty seven embryos were included from 203 cycle ICSI patients undergoing preimplantation genetic screening. The 23 chromosome pairs were analyzed by blastomere biopsy from day 3 embryos using a-CGH array method. Embryo development rate, fragmentation rate and chromosome status of the analyzed blastomeres were recorded and correlated with the aCGH results. Results: The incidence of chromosomal abnormalities was significantly higher in slow-and fast cleaving embryos at day 3 after insemination. The incidence of fragmentation and the type of fragmentation was associated with an increased incidence of chromosomal abnormalities. The symmetry of the blastomeres also correlated with the aneuploidy rates. Conclusions: Embryo development rate and morphological parameter such as degree, type of fragmentation and the symmetry of the blastomeres to a large extent reflect the cytogenetic status of the embryo and thus are important in the selection of embryos with the highest implantation potential. © 2014 Hainan Medical College. Source

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