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Tomasiello S.,University of Basilicata | Tomasiello S.,RD Laboratory
International Journal of Computer Mathematics | Year: 2010

In this paper, the Burgers-Huxley equation has been solved by a generalized version of the Iterative Differential Quadrature (IDQ) method for the first time. The IDQ method is a method based on the quadrature rules. It has been proposed by the author applying to a certain class of non-linear problems. Stability and error analysis are performed, showing the efficiency of the method. Besides, an error bound is tried. In the discussion about the numerical examples, the generalized Burgers-Huxley equation is involved too. Source


Seo J.H.,RD Laboratory | VanTyne C.J.,Colorado School of Mines | Moon Y.H.,Pusan National University
International Journal of Material Forming | Year: 2015

Turn down warping is a shape defect observed at the front end of a plate during hot rolling. To produce a flat plate without warping, a precise prediction of turn down warping is essential to achieve optimal control. Therefore, we propose a model based on a Gaussian function to predict turn down warping. The model uses the results from numerical analyses of hot plate rolling. The finite element code MARC was used for the numerical analysis. Hot plate rolling processing parameters, such as roll diameter, plate dimension, rolling speed, and pass line were all considered in the model. To verify the accuracy of the prediction model, the numerical results obtained by FEM were confirmed with data measured during industrial hot plate rolling. For the actual measurements of turn down warping, image processed high speed camera data from the exit side of the rolling were used. The results show that the proposed Gaussian function model can successfully predict turn down warping of a plate’s front end under various hot plate rolling conditions. © 2015 Springer-Verlag France Source


Mukherjee F.,RD Laboratory | Nagmani K.,Jawaharlal Nehru University | Prasad A.,RD Laboratory | Bahekar V.S.,RD Laboratory | And 6 more authors.
Current Trends in Biotechnology and Pharmacy | Year: 2015

Bovine brucellosis is a zoonotic disease. It impacts dairy industry since it is a major cause of abortion. The disease is caused by bacteria belonging to the genus Brucella. The pathogen is excreted in milk, semen and aborted materials. Bovine brucellosis is currently controlled by calfhood vaccination of females using live attenuated B. abortus strains (S19/RB51), however it does not provide 100% protection, and its use is restricted by age and gender. The vaccine strains are infectious to humans. Review of literature indicated that use of glyco-conjugate (GC) vaccines could address the above problems. Therefore, the objective of study was to assess the protective efficacy and therapeutic potential of GC vaccine prepared from Brucella abortus S19 (S19GC) in cattle. Immunization of Brucella free animals with two doses of 50μg each at an interval of 90 days by sub-cutaneous route resulted in pronounced Th1 mediated interferon gamma (IFN-γ) response (P=0.0061) as observed in ELISPOT assay compared to unvaccinated controls till 90 days post vaccination (DPV). The ability of S19GC to arrest shedding of Brucella was studied in brucellosis positive animals, vaccination of infected animals each with 50μg of S19GC by subcutaneous route resulted in arresting of shedding as evidenced by negative culture results, reduction (2 log folds) in genome copy number as observed by realtime PCR (qPCR). The prime-boost strategy of immunization (50 μg dose/ cattle) of a group of cattle infected with brucellosis (> 53%) was only able to arrest bacterial shedding transiently (7-60 DPV) in 66.67% immunized cattle, and after booster bacterial shedding was not recorded by culture in 83.34% of the animal till 250 PI. Also, Brucella genome was not detected transiently (7-150 DPV) in samples; but was detected thereafter till 250 DPV. The most promising effect of immunization with S19GC was observed in a group of cattle with a mixed population of brucellosis negative and positive animals of the above farm. Immunization of this group of animals with 100 μg of S19GC resulted in complete stoppage of shedding as indicated by culture and qPCR. The post immunization anti-LPS and anti-OMP antibody IgG1 and IgG2 response differed (P<0.01) at 30 DPV compared to pre immunization, suggesting the involvement of both Th2 and Th1 cells in the immunity conferred by S19GC vaccine. Ó 2015, Current Trends in Biotechnology and Pharmacy.All rights reserved. Source


Mukherjee F.,RD Laboratory | Bahekar V.S.,RD Laboratory | Bahekar V.S.,Jawaharlal Nehru Technological University | Prasad A.,RD Laboratory | And 4 more authors.
European Journal of Wildlife Research | Year: 2015

Tuberculosis (TB) in domestic and wild ruminants is mostly caused by Mycobacterium bovis (MB). However, the present paper describes the first report of TB of antelopes (Antelope cervicapra) and chinkara (Gazella bennettii) due to Mycobacterium tuberculosis (MT) in India. These wild hosts may represent a vehicle for the dissemination of MT infection to domestic livestock or human. MT was isolated by culture employing the MGITTM BACTECTM 960 (Becton Dickinson, BD) system and Middlebrook 7H10 Agar enriched with oleic albumin dextrose catalase (OADC) and Lowenstein-Jensen (LJ) medium supplemented with glycerol, but not sodium pyruvate. The isolates were confirmed as a member of the Mycobacterium tuberculosis complex (MTC) by using a commercial nested PCR that targeted the IS6110 sequence. Further confirmation of the isolates as MT strains was achieved by employing commercial line probe assay genotyping kits (Hain Lifescience, Germany) that specifically identifies MT within the MTC group. © 2015, Springer-Verlag Berlin Heidelberg. Source

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