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Zorkina Y.A.,rbsky State Research Center For Social And Forensic Psychiatry | Volgina N.E.,rbsky State Research Center For Social And Forensic Psychiatry | Gorlachev G.E.,N N Burdenko Research Institute Of Neurosurgery | Mel'nikov P.A.,Russian National Research Medical University | And 3 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2014

We studied the effect of γ-irradiation on HUVEC endothelial cells co-cultured with allogeneic astrocytes. This 2D in vitro model of the blood–brain barrier has the same parameters as cerebral microvascular endothelial cells forming the blood–brain barrier and allows reproducing its functions in vivo. Dose-dependent changes in cell morphology and violation of monolayer integrity were observed. Real-time PCR and immunocytochemical analysis revealed changes in the expression of tight (ZO-1, claudin-5) and adherens junction protein (vascular endothelial cadherin, β-catenin) mRNA. Expression of tight and adherens junction proteins mRNA decreased in 2, 24, and 48 h after irradiation in doses of 2, 4, and 6 Gy. Significant dosedependent changes were found only for β-catenin mRNA expression in 2 h after exposition. This model of blood–brain barrier in vitro can be used for studying the molecular mechanisms regulating permeability of cerebral endothelium under normal conditions and after pathological exposures, e.g. γ-irradiation. © 2014, Springer Science+Business Media New York. Source


Morozova A.Yu.,rbsky State Research Center For Social And Forensic Psychiatry | Zubkov E.A.,rbsky State Research Center For Social And Forensic Psychiatry | Storozheva Z.I.,Russian Academy of Medical Sciences | Kekelidze Z.I.,rbsky State Research Center For Social And Forensic Psychiatry | Chekhonin V.P.,Russian National Research Medical University
Bulletin of Experimental Biology and Medicine | Year: 2013

Behavioral reactions of rats were studied under conditions of constant exposure to ultrasonic irradiation at a frequency of 20-45 kHz for 21 days. In rats subjected to ultrasound, impaired behavior in the social interest test and Porsolt forced swimming test was observed; the development of anhedonia in the sucrose preference test was revealed (manifested in the symptoms of depression). The effects of ultrasonic irradiation were abolished by an antidepressant fluoxetine, a selective serotonin reuptake inhibitor. Our results indicate that chronic exposure to ultrasonic radiation produces a modulatory effect on depressive symptoms in rats. These changes satisfy the requirements for experimental animals, which are used as a model of psychopathological processes. © 2013 Springer Science+Business Media New York. Source


Abakumova T.O.,rbsky State Research Center For Social And Forensic Psychiatry | Kuz'kina A.A.,rbsky State Research Center For Social And Forensic Psychiatry | Zharova M.V.,rbsky State Research Center For Social And Forensic Psychiatry | Pozdeeva D.A.,rbsky State Research Center For Social And Forensic Psychiatry | And 6 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2015

To study demyelination and remyelination processes and their response to different drugs, a protocol for modeling multiple sclerosis using the copper chelator cuprizone was developed. Magnetic resonance imaging confirmed the presence of demyelination lesions on week 4 of 0.6% cuprizone-containing diet. Immunohistochemical staining with polyclonal antibodies to glial fibrillary acidic protein (pAb GFAP) confirmed the increase in the number of reactive astrocytes on week 4 of diet and during remyelination (week 2 after diet). Analysis of neurophysiological functions in mice with cuprizone-induced demyelination revealed motor and behavioral deficits. This model can be used as a tool for preclinical studies of the efficiency of multiple sclerosis diagnostic and therapy. © 2015, Springer Science+Business Media New York. Source

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