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Volgina N.E.,Russian National Research Medical University | Gurina O.I.,rbskii State Research Center For Social And Forensic Psychiatry | Grinenko N.F.,rbskii State Research Center For Social And Forensic Psychiatry | Baklaushev V.P.,Russian National Research Medical University | And 2 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2012

We studied expression of tight junction proteins and formation of the barrier properties in the culture of umbilical vein endothelial cells under conditions of co-culturing with allogenic GFAP-positive astrocytes. This culturing signifi cantly increases of expression of tight junction proteins (claudin-5, occludin, and ZO-1). The formation of tight junctions signifi cantly increased transendothelial resistance and reduced permeability for sodium fl uorescein. Thus, reproducible in vitro model for the study of endothelial barrier properties was created based on co-culturing of umbilical endothelial cells and human astrocytes. This model can be used for evaluation of the permeability of the tissue-blood barriers for substances of different chemical structure and for studies of factors modulating the state of cell-cell contacts. © 2012 Springer Science+Business Media New York. Source


Morozova A.Yu.,rbskii State Research Center For Social And Forensic Psychiatry | Zubkov E.A.,rbskii State Research Center For Social And Forensic Psychiatry | Koshkin F.A.,Russian National Research Medical University | Storozheva Z.I.,Russian Academy of Medical Sciences | Chekhonin V.P.,Russian National Research Medical University
Bulletin of Experimental Biology and Medicine | Year: 2014

The expression of genes encoding serotonin receptors and serotonin transporter in emotiogenic structures of rat brain was shown to change after chronic stress with ultrasonic waves of varying frequencies. A QPCR-RT study revealed the increased expression of genes for SERT and 5-HT1B receptor and decreased expression of the 5-HT2A receptor in the prefrontal cortex of rats after chronic exposure to ultrasonic waves of varying frequencies. Gene expression was increased for SERT, but decreased for 5-HT1B, 5-HT2A, and 5-HT2B receptors in the midbrain of animals. The hippocampus was characterized by an increased expression of genes encoding SERT and 5-HT1A, 5-HT2A, and 5-HT2B receptors. Our results indicate that the exposure to this type of stress is followed by dysregulation in the serotoninergic system of the brain. © 2014 Springer Science+Business Media New York. Source


Karasev A.V.,rbskii State Research Center For Social And Forensic Psychiatry | Lebedev S.V.,Moscow State University | Garats T.V.,rbskii State Research Center For Social And Forensic Psychiatry | Sharibzhanova A.M.,rbskii State Research Center For Social And Forensic Psychiatry | And 3 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2010

Seven-day old rats were subjected to unilateral ligation of the common carotid artery followed by 2-h exposure to a gas mixture consisting of 8% oxygen and 92% nitrogen. Locomotor function of rats was monitored weekly. Functional deficit in these animals persisted for at least 3 months. The exercise tests of rotarod, hanging, and narrowing track were most informative. Our results can be used in preclinical studies of new drugs for the therapy of perinatal brain injury. © 2010 Springer Science+Business Media, Inc. Source

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